Genotoxicity and Cytotoxicity Induced in Zygophyllum fabago by Low Pb Doses Depends on the Population’s Redox Plasticity

Lead (Pb) soil contamination remains a major ecological challenge. Zygophyllum fabago is a candidate for the Pb phytostabilisation of mining tailings; nevertheless, the cytogenotoxic effects of low doses of Pb on this species are still unknown. Therefore, Z. fabago</i&...

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Autores principales:	Antonio López-Orenes, Conceição Santos, Maria Celeste Dias, Helena Oliveira, María Á. Ferrer, Antonio A. Calderón, Sónia Silva
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	antioxidant response cytotoxicity genotoxicity Pb tolerance phytotoxicity Plant culture SB1-1110
Acceso en línea:	https://doaj.org/article/b50ff3fcacea48b4acc6154559b488f5
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spelling	oai:doaj.org-article:b50ff3fcacea48b4acc6154559b488f52021-11-25T17:47:20ZGenotoxicity and Cytotoxicity Induced in <i>Zygophyllum fabago</i> by Low Pb Doses Depends on the Population’s Redox Plasticity10.3390/horticulturae71104552311-7524https://doaj.org/article/b50ff3fcacea48b4acc6154559b488f52021-11-01T00:00:00Zhttps://www.mdpi.com/2311-7524/7/11/455https://doaj.org/toc/2311-7524Lead (Pb) soil contamination remains a major ecological challenge. <i>Zygophyllum fabago</i> is a candidate for the Pb phytostabilisation of mining tailings; nevertheless, the cytogenotoxic effects of low doses of Pb on this species are still unknown. Therefore, <i>Z. fabago</i> seeds collected from non-mining (NM) and mining (M) areas were exposed to 0, 5 and 20 µM Pb for four weeks, after which seedling growth, Pb cytogenotoxic effects and redox status were analyzed. The data revealed that Pb did not affect seedling growth in M populations, in contrast to the NM population. Cell cycle progression delay/arrest was detected in both NM and M seedlings, mostly in the roots. DNA damage (DNAd) was induced by Pb, particularly in NM seedlings. In contrast, M populations, which showed a higher Pb content, exhibited lower levels of DNAd and protein oxidation, together with higher levels of antioxidants. Upon Pb exposure, reduced glutathione (GSH) and non-protein thiols were upregulated in shoots and were unaffected/decreased in roots from the NM population, whereas M populations maintained higher levels of flavanols and hydroxycinnamic acids in shoots and triggered GSH in roots and shoots. These differential organ-specific mechanisms seem to be a competitive strategy that allows M populations to overcome Pb toxicity, contrarily to NM, thus stressing the importance of seed provenance in phytostabilisation programs.Antonio López-OrenesConceição SantosMaria Celeste DiasHelena OliveiraMaría Á. FerrerAntonio A. CalderónSónia SilvaMDPI AGarticleantioxidant responsecytotoxicitygenotoxicityPb tolerancephytotoxicityPlant cultureSB1-1110ENHorticulturae, Vol 7, Iss 455, p 455 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	antioxidant response cytotoxicity genotoxicity Pb tolerance phytotoxicity Plant culture SB1-1110
spellingShingle	antioxidant response cytotoxicity genotoxicity Pb tolerance phytotoxicity Plant culture SB1-1110 Antonio López-Orenes Conceição Santos Maria Celeste Dias Helena Oliveira María Á. Ferrer Antonio A. Calderón Sónia Silva Genotoxicity and Cytotoxicity Induced in <i>Zygophyllum fabago</i> by Low Pb Doses Depends on the Population’s Redox Plasticity
description	Lead (Pb) soil contamination remains a major ecological challenge. <i>Zygophyllum fabago</i> is a candidate for the Pb phytostabilisation of mining tailings; nevertheless, the cytogenotoxic effects of low doses of Pb on this species are still unknown. Therefore, <i>Z. fabago</i> seeds collected from non-mining (NM) and mining (M) areas were exposed to 0, 5 and 20 µM Pb for four weeks, after which seedling growth, Pb cytogenotoxic effects and redox status were analyzed. The data revealed that Pb did not affect seedling growth in M populations, in contrast to the NM population. Cell cycle progression delay/arrest was detected in both NM and M seedlings, mostly in the roots. DNA damage (DNAd) was induced by Pb, particularly in NM seedlings. In contrast, M populations, which showed a higher Pb content, exhibited lower levels of DNAd and protein oxidation, together with higher levels of antioxidants. Upon Pb exposure, reduced glutathione (GSH) and non-protein thiols were upregulated in shoots and were unaffected/decreased in roots from the NM population, whereas M populations maintained higher levels of flavanols and hydroxycinnamic acids in shoots and triggered GSH in roots and shoots. These differential organ-specific mechanisms seem to be a competitive strategy that allows M populations to overcome Pb toxicity, contrarily to NM, thus stressing the importance of seed provenance in phytostabilisation programs.
format	article
author	Antonio López-Orenes Conceição Santos Maria Celeste Dias Helena Oliveira María Á. Ferrer Antonio A. Calderón Sónia Silva
author_facet	Antonio López-Orenes Conceição Santos Maria Celeste Dias Helena Oliveira María Á. Ferrer Antonio A. Calderón Sónia Silva
author_sort	Antonio López-Orenes
title	Genotoxicity and Cytotoxicity Induced in <i>Zygophyllum fabago</i> by Low Pb Doses Depends on the Population’s Redox Plasticity
title_short	Genotoxicity and Cytotoxicity Induced in <i>Zygophyllum fabago</i> by Low Pb Doses Depends on the Population’s Redox Plasticity
title_full	Genotoxicity and Cytotoxicity Induced in <i>Zygophyllum fabago</i> by Low Pb Doses Depends on the Population’s Redox Plasticity
title_fullStr	Genotoxicity and Cytotoxicity Induced in <i>Zygophyllum fabago</i> by Low Pb Doses Depends on the Population’s Redox Plasticity
title_full_unstemmed	Genotoxicity and Cytotoxicity Induced in <i>Zygophyllum fabago</i> by Low Pb Doses Depends on the Population’s Redox Plasticity
title_sort	genotoxicity and cytotoxicity induced in <i>zygophyllum fabago</i> by low pb doses depends on the population’s redox plasticity
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/b50ff3fcacea48b4acc6154559b488f5
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Genotoxicity and Cytotoxicity Induced in <i>Zygophyllum fabago</i> by Low Pb Doses Depends on the Population’s Redox Plasticity

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