MCT2 expression and lactate influx in anorexigenic and orexigenic neurons of the arcuate nucleus.

Hypothalamic neurons of the arcuate nucleus control food intake, releasing orexigenic and anorexigenic neuropeptides in response to changes in glucose concentration. Several studies have suggested that the glucosensing mechanism is governed by a metabolic interaction between neurons and glial cells...

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Autores principales:	Christian Cortes-Campos, Roberto Elizondo, Claudio Carril, Fernando Martínez, Katica Boric, Francisco Nualart, Maria Angeles Garcia-Robles
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2013
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/b51427970c6f4b1792b92bfafe054caf
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spelling	oai:doaj.org-article:b51427970c6f4b1792b92bfafe054caf2021-11-18T07:47:43ZMCT2 expression and lactate influx in anorexigenic and orexigenic neurons of the arcuate nucleus.1932-620310.1371/journal.pone.0062532https://doaj.org/article/b51427970c6f4b1792b92bfafe054caf2013-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/23638108/?tool=EBIhttps://doaj.org/toc/1932-6203Hypothalamic neurons of the arcuate nucleus control food intake, releasing orexigenic and anorexigenic neuropeptides in response to changes in glucose concentration. Several studies have suggested that the glucosensing mechanism is governed by a metabolic interaction between neurons and glial cells via lactate flux through monocarboxylate transporters (MCTs). Hypothalamic glial cells (tanycytes) release lactate through MCT1 and MCT4; however, similar analyses in neuroendocrine neurons have yet to be undertaken. Using primary rat hypothalamic cell cultures and fluorimetric assays, lactate incorporation was detected. Furthermore, the expression and function of MCT2 was demonstrated in the hypothalamic neuronal cell line, GT1-7, using kinetic and inhibition assays. Moreover, MCT2 expression and localization in the Sprague Dawley rat hypothalamus was analyzed using RT-PCR, in situ hybridization and Western blot analyses. Confocal immunohistochemistry analyses revealed MCT2 localization in neuronal but not glial cells. Moreover, MCT2 was localized to ∼90% of orexigenic and ~60% of anorexigenic neurons as determined by immunolocalization analysis of AgRP and POMC with MCT2-positives neurons. Thus, MCT2 distribution coupled with lactate uptake by hypothalamic neurons suggests that hypothalamic neurons control food intake using lactate to reflect changes in glucose levels.Christian Cortes-CamposRoberto ElizondoClaudio CarrilFernando MartínezKatica BoricFrancisco NualartMaria Angeles Garcia-RoblesPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 8, Iss 4, p e62532 (2013)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Christian Cortes-Campos Roberto Elizondo Claudio Carril Fernando Martínez Katica Boric Francisco Nualart Maria Angeles Garcia-Robles MCT2 expression and lactate influx in anorexigenic and orexigenic neurons of the arcuate nucleus.
description	Hypothalamic neurons of the arcuate nucleus control food intake, releasing orexigenic and anorexigenic neuropeptides in response to changes in glucose concentration. Several studies have suggested that the glucosensing mechanism is governed by a metabolic interaction between neurons and glial cells via lactate flux through monocarboxylate transporters (MCTs). Hypothalamic glial cells (tanycytes) release lactate through MCT1 and MCT4; however, similar analyses in neuroendocrine neurons have yet to be undertaken. Using primary rat hypothalamic cell cultures and fluorimetric assays, lactate incorporation was detected. Furthermore, the expression and function of MCT2 was demonstrated in the hypothalamic neuronal cell line, GT1-7, using kinetic and inhibition assays. Moreover, MCT2 expression and localization in the Sprague Dawley rat hypothalamus was analyzed using RT-PCR, in situ hybridization and Western blot analyses. Confocal immunohistochemistry analyses revealed MCT2 localization in neuronal but not glial cells. Moreover, MCT2 was localized to ∼90% of orexigenic and ~60% of anorexigenic neurons as determined by immunolocalization analysis of AgRP and POMC with MCT2-positives neurons. Thus, MCT2 distribution coupled with lactate uptake by hypothalamic neurons suggests that hypothalamic neurons control food intake using lactate to reflect changes in glucose levels.
format	article
author	Christian Cortes-Campos Roberto Elizondo Claudio Carril Fernando Martínez Katica Boric Francisco Nualart Maria Angeles Garcia-Robles
author_facet	Christian Cortes-Campos Roberto Elizondo Claudio Carril Fernando Martínez Katica Boric Francisco Nualart Maria Angeles Garcia-Robles
author_sort	Christian Cortes-Campos
title	MCT2 expression and lactate influx in anorexigenic and orexigenic neurons of the arcuate nucleus.
title_short	MCT2 expression and lactate influx in anorexigenic and orexigenic neurons of the arcuate nucleus.
title_full	MCT2 expression and lactate influx in anorexigenic and orexigenic neurons of the arcuate nucleus.
title_fullStr	MCT2 expression and lactate influx in anorexigenic and orexigenic neurons of the arcuate nucleus.
title_full_unstemmed	MCT2 expression and lactate influx in anorexigenic and orexigenic neurons of the arcuate nucleus.
title_sort	mct2 expression and lactate influx in anorexigenic and orexigenic neurons of the arcuate nucleus.
publisher	Public Library of Science (PLoS)
publishDate	2013
url	https://doaj.org/article/b51427970c6f4b1792b92bfafe054caf
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MCT2 expression and lactate influx in anorexigenic and orexigenic neurons of the arcuate nucleus.

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