Yeast surface display identifies a family of evasins from ticks with novel polyvalent CC chemokine-binding activities

Abstract Chemokines function via G-protein coupled receptors in a robust network to recruit immune cells to sites of inflammation. Due to the complexity of this network, targeting single chemokines or receptors has not been successful in inflammatory disease. Dog tick saliva contains polyvalent CC-c...

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Autores principales:	Kamayani Singh, Graham Davies, Yara Alenazi, James R. O. Eaton, Akane Kawamura, Shoumo Bhattacharya
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/b514a9c56ad24d53bfea2339ddae7894
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spelling	oai:doaj.org-article:b514a9c56ad24d53bfea2339ddae78942021-12-02T12:32:03ZYeast surface display identifies a family of evasins from ticks with novel polyvalent CC chemokine-binding activities10.1038/s41598-017-04378-12045-2322https://doaj.org/article/b514a9c56ad24d53bfea2339ddae78942017-06-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-04378-1https://doaj.org/toc/2045-2322Abstract Chemokines function via G-protein coupled receptors in a robust network to recruit immune cells to sites of inflammation. Due to the complexity of this network, targeting single chemokines or receptors has not been successful in inflammatory disease. Dog tick saliva contains polyvalent CC-chemokine binding peptides termed evasins 1 and 4, that efficiently disrupt the chemokine network in models of inflammatory disease. Here we develop yeast surface display as a tool for functionally identifying evasins, and use it to identify 10 novel polyvalent CC-chemokine binding evasin-like peptides from salivary transcriptomes of eight tick species in Rhipicephalus and Amblyomma genera. These evasins have unique binding profiles compared to evasins 1 and 4, targeting CCL2 and CCL13 in addition to other CC-chemokines. Evasin binding leads to neutralisation of chemokine function including that of complex chemokine mixtures, suggesting therapeutic efficacy in inflammatory disease. We propose that yeast surface display is a powerful approach to mine potential therapeutics from inter-species protein interactions that have arisen during evolution of parasitism in ticks.Kamayani SinghGraham DaviesYara AlenaziJames R. O. EatonAkane KawamuraShoumo BhattacharyaNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-15 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Kamayani Singh Graham Davies Yara Alenazi James R. O. Eaton Akane Kawamura Shoumo Bhattacharya Yeast surface display identifies a family of evasins from ticks with novel polyvalent CC chemokine-binding activities
description	Abstract Chemokines function via G-protein coupled receptors in a robust network to recruit immune cells to sites of inflammation. Due to the complexity of this network, targeting single chemokines or receptors has not been successful in inflammatory disease. Dog tick saliva contains polyvalent CC-chemokine binding peptides termed evasins 1 and 4, that efficiently disrupt the chemokine network in models of inflammatory disease. Here we develop yeast surface display as a tool for functionally identifying evasins, and use it to identify 10 novel polyvalent CC-chemokine binding evasin-like peptides from salivary transcriptomes of eight tick species in Rhipicephalus and Amblyomma genera. These evasins have unique binding profiles compared to evasins 1 and 4, targeting CCL2 and CCL13 in addition to other CC-chemokines. Evasin binding leads to neutralisation of chemokine function including that of complex chemokine mixtures, suggesting therapeutic efficacy in inflammatory disease. We propose that yeast surface display is a powerful approach to mine potential therapeutics from inter-species protein interactions that have arisen during evolution of parasitism in ticks.
format	article
author	Kamayani Singh Graham Davies Yara Alenazi James R. O. Eaton Akane Kawamura Shoumo Bhattacharya
author_facet	Kamayani Singh Graham Davies Yara Alenazi James R. O. Eaton Akane Kawamura Shoumo Bhattacharya
author_sort	Kamayani Singh
title	Yeast surface display identifies a family of evasins from ticks with novel polyvalent CC chemokine-binding activities
title_short	Yeast surface display identifies a family of evasins from ticks with novel polyvalent CC chemokine-binding activities
title_full	Yeast surface display identifies a family of evasins from ticks with novel polyvalent CC chemokine-binding activities
title_fullStr	Yeast surface display identifies a family of evasins from ticks with novel polyvalent CC chemokine-binding activities
title_full_unstemmed	Yeast surface display identifies a family of evasins from ticks with novel polyvalent CC chemokine-binding activities
title_sort	yeast surface display identifies a family of evasins from ticks with novel polyvalent cc chemokine-binding activities
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/b514a9c56ad24d53bfea2339ddae7894
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Yeast surface display identifies a family of evasins from ticks with novel polyvalent CC chemokine-binding activities

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