Steroid Receptor Coregulators Can Modulate the Action of Progesterone Receptor during the Estrous Cycle in Cow Endometrium

Nuclear receptor coregulators include coactivators and corepressors which associate with the progesterone receptor (PGR) during its activation. Fluctuations in the transcription levels of their respective genes and subsequent protein production as well as in related activities for histone acetyltran...

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Autores principales:	Robert Rekawiecki, Karolina Dobrzyn, Magdalena K. Kowalik
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	progesterone receptor coregulators P300 CREB SRC-1 NCOR-2 endometrium Veterinary medicine SF600-1100 Zoology QL1-991
Acceso en línea:	https://doaj.org/article/b517303f0f114b3fb07614c7a7fb0f30
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id	oai:doaj.org-article:b517303f0f114b3fb07614c7a7fb0f30
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spelling	oai:doaj.org-article:b517303f0f114b3fb07614c7a7fb0f302021-11-25T16:19:21ZSteroid Receptor Coregulators Can Modulate the Action of Progesterone Receptor during the Estrous Cycle in Cow Endometrium10.3390/ani111132172076-2615https://doaj.org/article/b517303f0f114b3fb07614c7a7fb0f302021-11-01T00:00:00Zhttps://www.mdpi.com/2076-2615/11/11/3217https://doaj.org/toc/2076-2615Nuclear receptor coregulators include coactivators and corepressors which associate with the progesterone receptor (PGR) during its activation. Fluctuations in the transcription levels of their respective genes and subsequent protein production as well as in related activities for histone acetyltransferase (HAT) and histone deacetylase (HDAC) can affect PGR function and thus change the action of progesterone (P4) in bovine endometrium during the estrous cycle. Endometrial tissue on days 2–5, 6–10, 11–16, and 17–20 of the estrous cycle was used for determination of the mRNA expression levels of coactivators P300, CREB, and SRC-1 along with corepressor NCOR-2 using Real-Time PCR, with protein levels by Western blot. Coregulators cellular localizations were assessed by immunohistochemistry whereas the activities of HAT and HDAC by using EIA. The highest levels of mRNA and proteins for all of the investigated coregulators, as well as the highest levels of activity for HAT and HDAC, were detected over days 2–16 of the estrous cycle. All of the tested coregulatory proteins were localized in the nuclei of endometrial cells. This research indicates the important role of coregulators of the PGR receptor in regulating P4 activity in endometrial cells, especially during the pre-implantation period.Robert RekawieckiKarolina DobrzynMagdalena K. KowalikMDPI AGarticleprogesterone receptor coregulatorsP300CREBSRC-1NCOR-2endometriumVeterinary medicineSF600-1100ZoologyQL1-991ENAnimals, Vol 11, Iss 3217, p 3217 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	progesterone receptor coregulators P300 CREB SRC-1 NCOR-2 endometrium Veterinary medicine SF600-1100 Zoology QL1-991
spellingShingle	progesterone receptor coregulators P300 CREB SRC-1 NCOR-2 endometrium Veterinary medicine SF600-1100 Zoology QL1-991 Robert Rekawiecki Karolina Dobrzyn Magdalena K. Kowalik Steroid Receptor Coregulators Can Modulate the Action of Progesterone Receptor during the Estrous Cycle in Cow Endometrium
description	Nuclear receptor coregulators include coactivators and corepressors which associate with the progesterone receptor (PGR) during its activation. Fluctuations in the transcription levels of their respective genes and subsequent protein production as well as in related activities for histone acetyltransferase (HAT) and histone deacetylase (HDAC) can affect PGR function and thus change the action of progesterone (P4) in bovine endometrium during the estrous cycle. Endometrial tissue on days 2–5, 6–10, 11–16, and 17–20 of the estrous cycle was used for determination of the mRNA expression levels of coactivators P300, CREB, and SRC-1 along with corepressor NCOR-2 using Real-Time PCR, with protein levels by Western blot. Coregulators cellular localizations were assessed by immunohistochemistry whereas the activities of HAT and HDAC by using EIA. The highest levels of mRNA and proteins for all of the investigated coregulators, as well as the highest levels of activity for HAT and HDAC, were detected over days 2–16 of the estrous cycle. All of the tested coregulatory proteins were localized in the nuclei of endometrial cells. This research indicates the important role of coregulators of the PGR receptor in regulating P4 activity in endometrial cells, especially during the pre-implantation period.
format	article
author	Robert Rekawiecki Karolina Dobrzyn Magdalena K. Kowalik
author_facet	Robert Rekawiecki Karolina Dobrzyn Magdalena K. Kowalik
author_sort	Robert Rekawiecki
title	Steroid Receptor Coregulators Can Modulate the Action of Progesterone Receptor during the Estrous Cycle in Cow Endometrium
title_short	Steroid Receptor Coregulators Can Modulate the Action of Progesterone Receptor during the Estrous Cycle in Cow Endometrium
title_full	Steroid Receptor Coregulators Can Modulate the Action of Progesterone Receptor during the Estrous Cycle in Cow Endometrium
title_fullStr	Steroid Receptor Coregulators Can Modulate the Action of Progesterone Receptor during the Estrous Cycle in Cow Endometrium
title_full_unstemmed	Steroid Receptor Coregulators Can Modulate the Action of Progesterone Receptor during the Estrous Cycle in Cow Endometrium
title_sort	steroid receptor coregulators can modulate the action of progesterone receptor during the estrous cycle in cow endometrium
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/b517303f0f114b3fb07614c7a7fb0f30
work_keys_str_mv	AT robertrekawiecki steroidreceptorcoregulatorscanmodulatetheactionofprogesteronereceptorduringtheestrouscycleincowendometrium AT karolinadobrzyn steroidreceptorcoregulatorscanmodulatetheactionofprogesteronereceptorduringtheestrouscycleincowendometrium AT magdalenakkowalik steroidreceptorcoregulatorscanmodulatetheactionofprogesteronereceptorduringtheestrouscycleincowendometrium
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Steroid Receptor Coregulators Can Modulate the Action of Progesterone Receptor during the Estrous Cycle in Cow Endometrium

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