Fabrication and in vitro Evaluation of 4-HIA Encapsulated PLGA Nanoparticles on PC12 Cells

Tekupalli Ravikiran,1 Santosh Anand,2 Mohammad Azam Ansari,3 Mohammad N Alomary,4 Sami AlYahya,4 Sowbhagya Ramachandregowda,1 Saad Alghamdi,5 Anupama Sindhghatta Kariyappa,1 Bhagyalakshmi Dundaiah,1 Mamatha Madhugiri Gopinath,1 Sumreen Sultana,1 Shital Manohar Punekar,1 Thimappa Ramachandrappa Laksh...

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Autores principales:	Ravikiran T, Anand S, Ansari MA, Alomary MN, AlYahya S, Ramachandregowda S, Alghamdi S, Sindhghatta Kariyappa A, Dundaiah B, Madhugiri Gopinath M, Sultana S, Punekar SM, Lakshmeesha TR
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2021
Materias:	4-hydroxyisopthalic acid polylactic-co-glycolic acid nanoencapsulation pc12 cells cytotoxicity Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/b51fbc8dd1934ec1a6cc5e418646118e
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spelling	oai:doaj.org-article:b51fbc8dd1934ec1a6cc5e418646118e2021-12-02T16:39:47ZFabrication and in vitro Evaluation of 4-HIA Encapsulated PLGA Nanoparticles on PC12 Cells1178-2013https://doaj.org/article/b51fbc8dd1934ec1a6cc5e418646118e2021-08-01T00:00:00Zhttps://www.dovepress.com/fabrication-and-in-vitro-evaluation-of-4-hia-encapsulated-plga-nanopar-peer-reviewed-fulltext-article-IJNhttps://doaj.org/toc/1178-2013Tekupalli Ravikiran,1 Santosh Anand,2 Mohammad Azam Ansari,3 Mohammad N Alomary,4 Sami AlYahya,4 Sowbhagya Ramachandregowda,1 Saad Alghamdi,5 Anupama Sindhghatta Kariyappa,1 Bhagyalakshmi Dundaiah,1 Mamatha Madhugiri Gopinath,1 Sumreen Sultana,1 Shital Manohar Punekar,1 Thimappa Ramachandrappa Lakshmeesha1 1Department of Biotechnology, Bangalore University, Bangalore, India; 2Department of Biotechnology, School of Applied Sciences, Reva University, Bengaluru, India; 3Department of Epidemic Disease Research, Institutes for Research and Medical Consultations (IRMC), Imam Abdulrahman Bin Faisal University, Dammam, 31441, Saudi Arabia; 4National Center for Biotechnology, Life Science and Environmental Research Institute, King Abdulaziz City for Science and Technology, Riyadh, Saudi Arabia; 5Laboratory Medicine Department, Faculty of Applied Medical Sciences, Umm Al-Qura University, Makkah, Saudi ArabiaCorrespondence: Thimappa Ramachandrappa LakshmeeshaDepartment of Biotechnology, Bangalore University, Bangalore, 560056, Karnataka, IndiaTel +91 8710937464Email lakshmeeshat6@gmail.comMohammad Azam AnsariDepartment of Epidemic Disease Research, Institutes for Research and Medical Consultations (IRMC), Imam Abdulrahman Bin Faisal University, Dammam, 31441, Saudi ArabiaTel +966 580306835Email maansari@iau.edu.saPurpose: 4-Hydroxyisophthalic acid (4-HIA) is a bioactive compound present in the roots of Decalepis hamiltonii, which has attracted considerable attention in attenuating oxidative stress-related neurodegenerative diseases. However, its efficacy is limited because of its low solubility and bioavailability. Therefore, the present study aimed to encapsulate 4-HIA using biocompatible copolymer polylactide-co-glycolide (PLGA) and evaluate its antioxidant and neuroprotective potential.Methods: The nanoparticles (NPs) were fabricated by solid/oil/water (s/o/w) emulsion technique and characterized using XRD, SEM, HR-TEM, and FTIR spectroscopy. Antioxidant assays such as 1,1 diphenyl-2-picrylhydrazyl (DPPH), superoxide, and hydroxyl radical scavenging ability were performed to assess the antioxidant potential of the fabricated NPs.Results: The bioactive component, 4-HIA, was efficiently encapsulated by the PLGA polymer and was found to be spherical and smooth with a size < 10nm. 4-HIA showed better scavenging capability in DPPH and superoxide assays as compared to 4-HIA encapsulated PLGA and butylated hydroxytoluene (BHT). In contrast, 4-HIA encapsulated PLGA NPs exhibited a significant hydroxyl radical scavenging activity than 4-HIA and BHT alone. Further, the encapsulated NPs efficiently curtailed hydrogen peroxide (H2O2)-induced cytotoxicity in PC12 cells.Conclusion: Our findings indicate that 4-HIA encapsulated PLGA NPs might be a therapeutic intervention towards the effective management of oxidative stress as it has exhibited efficient neuroprotective potential against H2O2-induced oxidative stress in PC12 cells.Keywords: 4-Hydroxyisophthalic acid, polylactic-co-glycolic acid, nanoencapsulation, PC12 cells, cytotoxicityRavikiran TAnand SAnsari MAAlomary MNAlYahya SRamachandregowda SAlghamdi SSindhghatta Kariyappa ADundaiah BMadhugiri Gopinath MSultana SPunekar SMLakshmeesha TRDove Medical Pressarticle4-hydroxyisopthalic acidpolylactic-co-glycolic acidnanoencapsulationpc12 cellscytotoxicityMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol Volume 16, Pp 5621-5632 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	4-hydroxyisopthalic acid polylactic-co-glycolic acid nanoencapsulation pc12 cells cytotoxicity Medicine (General) R5-920
spellingShingle	4-hydroxyisopthalic acid polylactic-co-glycolic acid nanoencapsulation pc12 cells cytotoxicity Medicine (General) R5-920 Ravikiran T Anand S Ansari MA Alomary MN AlYahya S Ramachandregowda S Alghamdi S Sindhghatta Kariyappa A Dundaiah B Madhugiri Gopinath M Sultana S Punekar SM Lakshmeesha TR Fabrication and in vitro Evaluation of 4-HIA Encapsulated PLGA Nanoparticles on PC12 Cells
description	Tekupalli Ravikiran,1 Santosh Anand,2 Mohammad Azam Ansari,3 Mohammad N Alomary,4 Sami AlYahya,4 Sowbhagya Ramachandregowda,1 Saad Alghamdi,5 Anupama Sindhghatta Kariyappa,1 Bhagyalakshmi Dundaiah,1 Mamatha Madhugiri Gopinath,1 Sumreen Sultana,1 Shital Manohar Punekar,1 Thimappa Ramachandrappa Lakshmeesha1 1Department of Biotechnology, Bangalore University, Bangalore, India; 2Department of Biotechnology, School of Applied Sciences, Reva University, Bengaluru, India; 3Department of Epidemic Disease Research, Institutes for Research and Medical Consultations (IRMC), Imam Abdulrahman Bin Faisal University, Dammam, 31441, Saudi Arabia; 4National Center for Biotechnology, Life Science and Environmental Research Institute, King Abdulaziz City for Science and Technology, Riyadh, Saudi Arabia; 5Laboratory Medicine Department, Faculty of Applied Medical Sciences, Umm Al-Qura University, Makkah, Saudi ArabiaCorrespondence: Thimappa Ramachandrappa LakshmeeshaDepartment of Biotechnology, Bangalore University, Bangalore, 560056, Karnataka, IndiaTel +91 8710937464Email lakshmeeshat6@gmail.comMohammad Azam AnsariDepartment of Epidemic Disease Research, Institutes for Research and Medical Consultations (IRMC), Imam Abdulrahman Bin Faisal University, Dammam, 31441, Saudi ArabiaTel +966 580306835Email maansari@iau.edu.saPurpose: 4-Hydroxyisophthalic acid (4-HIA) is a bioactive compound present in the roots of Decalepis hamiltonii, which has attracted considerable attention in attenuating oxidative stress-related neurodegenerative diseases. However, its efficacy is limited because of its low solubility and bioavailability. Therefore, the present study aimed to encapsulate 4-HIA using biocompatible copolymer polylactide-co-glycolide (PLGA) and evaluate its antioxidant and neuroprotective potential.Methods: The nanoparticles (NPs) were fabricated by solid/oil/water (s/o/w) emulsion technique and characterized using XRD, SEM, HR-TEM, and FTIR spectroscopy. Antioxidant assays such as 1,1 diphenyl-2-picrylhydrazyl (DPPH), superoxide, and hydroxyl radical scavenging ability were performed to assess the antioxidant potential of the fabricated NPs.Results: The bioactive component, 4-HIA, was efficiently encapsulated by the PLGA polymer and was found to be spherical and smooth with a size < 10nm. 4-HIA showed better scavenging capability in DPPH and superoxide assays as compared to 4-HIA encapsulated PLGA and butylated hydroxytoluene (BHT). In contrast, 4-HIA encapsulated PLGA NPs exhibited a significant hydroxyl radical scavenging activity than 4-HIA and BHT alone. Further, the encapsulated NPs efficiently curtailed hydrogen peroxide (H2O2)-induced cytotoxicity in PC12 cells.Conclusion: Our findings indicate that 4-HIA encapsulated PLGA NPs might be a therapeutic intervention towards the effective management of oxidative stress as it has exhibited efficient neuroprotective potential against H2O2-induced oxidative stress in PC12 cells.Keywords: 4-Hydroxyisophthalic acid, polylactic-co-glycolic acid, nanoencapsulation, PC12 cells, cytotoxicity
format	article
author	Ravikiran T Anand S Ansari MA Alomary MN AlYahya S Ramachandregowda S Alghamdi S Sindhghatta Kariyappa A Dundaiah B Madhugiri Gopinath M Sultana S Punekar SM Lakshmeesha TR
author_facet	Ravikiran T Anand S Ansari MA Alomary MN AlYahya S Ramachandregowda S Alghamdi S Sindhghatta Kariyappa A Dundaiah B Madhugiri Gopinath M Sultana S Punekar SM Lakshmeesha TR
author_sort	Ravikiran T
title	Fabrication and in vitro Evaluation of 4-HIA Encapsulated PLGA Nanoparticles on PC12 Cells
title_short	Fabrication and in vitro Evaluation of 4-HIA Encapsulated PLGA Nanoparticles on PC12 Cells
title_full	Fabrication and in vitro Evaluation of 4-HIA Encapsulated PLGA Nanoparticles on PC12 Cells
title_fullStr	Fabrication and in vitro Evaluation of 4-HIA Encapsulated PLGA Nanoparticles on PC12 Cells
title_full_unstemmed	Fabrication and in vitro Evaluation of 4-HIA Encapsulated PLGA Nanoparticles on PC12 Cells
title_sort	fabrication and in vitro evaluation of 4-hia encapsulated plga nanoparticles on pc12 cells
publisher	Dove Medical Press
publishDate	2021
url	https://doaj.org/article/b51fbc8dd1934ec1a6cc5e418646118e
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Fabrication and in vitro Evaluation of 4-HIA Encapsulated PLGA Nanoparticles on PC12 Cells

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