Estrogens Regulate Placental Angiogenesis in Horses

A sufficient vascular network within the feto-maternal interface is necessary for placental function. Several pregnancy abnormalities have been associated with abnormal vascular formations in the placenta. We hypothesized that growth and expansion of the placental vascular network in the equine (<...

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Autores principales:	Shingo Haneda, Pouya Dini, Alejandro Esteller-Vico, Kirsten E. Scoggin, Edward L. Squires, Mats H. Troedsson, Peter Daels, Yasuo Nambo, Barry A. Ball
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	chorioallantois placenta letrozole angiogenesis placental development estrogen Biology (General) QH301-705.5 Chemistry QD1-999
Acceso en línea:	https://doaj.org/article/b5250dc5ce064502b62427bb1f23a405
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spelling	oai:doaj.org-article:b5250dc5ce064502b62427bb1f23a4052021-11-25T17:53:30ZEstrogens Regulate Placental Angiogenesis in Horses10.3390/ijms2222121161422-00671661-6596https://doaj.org/article/b5250dc5ce064502b62427bb1f23a4052021-11-01T00:00:00Zhttps://www.mdpi.com/1422-0067/22/22/12116https://doaj.org/toc/1661-6596https://doaj.org/toc/1422-0067A sufficient vascular network within the feto-maternal interface is necessary for placental function. Several pregnancy abnormalities have been associated with abnormal vascular formations in the placenta. We hypothesized that growth and expansion of the placental vascular network in the equine (<i>Equus caballus</i>) placenta is regulated by estrogens (estrogen family hormones), a hormone with a high circulating concentration during equine gestation. Administration of letrozole, a potent and specific inhibitor of aromatase, during the first trimester (D30 to D118), decreased circulatory estrone sulfate concentrations, increased circulatory testosterone and androstenedione concentrations, and tended to reduce the weight of the fetus (<i>p</i> < 0.1). Moreover, the gene expression of <i>CYP17A1</i> was increased, and the expression of androgen receptor was decreased in the D120 chorioallantois (CA) of letrozole-treated mares in comparison to that of the control mares. We also found that at D120, the number of vessels tended to decrease in the CAs with letrozole treatment (<i>p</i> = 0.07). In addition, expression of a subset of angiogenic genes, such as <i>ANGPT1</i>, <i>VEGF</i>, and <i>NOS2</i>, were altered in the CAs of letrozole-treated mares. We further demonstrated that 17β-estradiol increases the expression of <i>ANGPT1</i> and <i>VEGF</i> and increases the angiogenic activity of equine endothelial cells in vitro. Our results from the estrogen-suppressed group demonstrated an impaired placental vascular network, suggesting an estrogen-dependent vasculogenesis in the equine CA during the first trimester.Shingo HanedaPouya DiniAlejandro Esteller-VicoKirsten E. ScogginEdward L. SquiresMats H. TroedssonPeter DaelsYasuo NamboBarry A. BallMDPI AGarticlechorioallantoisplacentaletrozoleangiogenesisplacental developmentestrogenBiology (General)QH301-705.5ChemistryQD1-999ENInternational Journal of Molecular Sciences, Vol 22, Iss 12116, p 12116 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	chorioallantois placenta letrozole angiogenesis placental development estrogen Biology (General) QH301-705.5 Chemistry QD1-999
spellingShingle	chorioallantois placenta letrozole angiogenesis placental development estrogen Biology (General) QH301-705.5 Chemistry QD1-999 Shingo Haneda Pouya Dini Alejandro Esteller-Vico Kirsten E. Scoggin Edward L. Squires Mats H. Troedsson Peter Daels Yasuo Nambo Barry A. Ball Estrogens Regulate Placental Angiogenesis in Horses
description	A sufficient vascular network within the feto-maternal interface is necessary for placental function. Several pregnancy abnormalities have been associated with abnormal vascular formations in the placenta. We hypothesized that growth and expansion of the placental vascular network in the equine (<i>Equus caballus</i>) placenta is regulated by estrogens (estrogen family hormones), a hormone with a high circulating concentration during equine gestation. Administration of letrozole, a potent and specific inhibitor of aromatase, during the first trimester (D30 to D118), decreased circulatory estrone sulfate concentrations, increased circulatory testosterone and androstenedione concentrations, and tended to reduce the weight of the fetus (<i>p</i> < 0.1). Moreover, the gene expression of <i>CYP17A1</i> was increased, and the expression of androgen receptor was decreased in the D120 chorioallantois (CA) of letrozole-treated mares in comparison to that of the control mares. We also found that at D120, the number of vessels tended to decrease in the CAs with letrozole treatment (<i>p</i> = 0.07). In addition, expression of a subset of angiogenic genes, such as <i>ANGPT1</i>, <i>VEGF</i>, and <i>NOS2</i>, were altered in the CAs of letrozole-treated mares. We further demonstrated that 17β-estradiol increases the expression of <i>ANGPT1</i> and <i>VEGF</i> and increases the angiogenic activity of equine endothelial cells in vitro. Our results from the estrogen-suppressed group demonstrated an impaired placental vascular network, suggesting an estrogen-dependent vasculogenesis in the equine CA during the first trimester.
format	article
author	Shingo Haneda Pouya Dini Alejandro Esteller-Vico Kirsten E. Scoggin Edward L. Squires Mats H. Troedsson Peter Daels Yasuo Nambo Barry A. Ball
author_facet	Shingo Haneda Pouya Dini Alejandro Esteller-Vico Kirsten E. Scoggin Edward L. Squires Mats H. Troedsson Peter Daels Yasuo Nambo Barry A. Ball
author_sort	Shingo Haneda
title	Estrogens Regulate Placental Angiogenesis in Horses
title_short	Estrogens Regulate Placental Angiogenesis in Horses
title_full	Estrogens Regulate Placental Angiogenesis in Horses
title_fullStr	Estrogens Regulate Placental Angiogenesis in Horses
title_full_unstemmed	Estrogens Regulate Placental Angiogenesis in Horses
title_sort	estrogens regulate placental angiogenesis in horses
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/b5250dc5ce064502b62427bb1f23a405
work_keys_str_mv	AT shingohaneda estrogensregulateplacentalangiogenesisinhorses AT pouyadini estrogensregulateplacentalangiogenesisinhorses AT alejandroestellervico estrogensregulateplacentalangiogenesisinhorses AT kirstenescoggin estrogensregulateplacentalangiogenesisinhorses AT edwardlsquires estrogensregulateplacentalangiogenesisinhorses AT matshtroedsson estrogensregulateplacentalangiogenesisinhorses AT peterdaels estrogensregulateplacentalangiogenesisinhorses AT yasuonambo estrogensregulateplacentalangiogenesisinhorses AT barryaball estrogensregulateplacentalangiogenesisinhorses
_version_	1718411882243555328

Estrogens Regulate Placental Angiogenesis in Horses

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