Evaluation of Anticancer Bioactive Compounds and Cytotoxicity of Kaffir Lime (Citrus hystrix Dc.) Callus Extract Post Preservation

Previous studies have shown that kaffir lime extract is toxic to breast cancer T47D cells. To increase the bioactive compound's production for traditional cancer medicine raw material, we induce kaffir lime callus in vitro. One strategy to continuously produce kaffir lime callus is by using cal...

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Autores principales:	Woro Anindito Sri Tunjung, Sudewi Fajarina, Beni Hendro Prabowo, Frisca Damayanti, Asti Widyasari, Aries Bagus Sasongko, Ari Indrianto, Endang Semiarti, Lisna Hidayati
Formato:	article
Lenguaje:	EN
Publicado:	Universitas Gadjah Mada 2021
Materias:	kaffir lime callus, preservation, cytotoxicity, bioactive compounds Pharmacy and materia medica RS1-441
Acceso en línea:	https://doaj.org/article/b5272b6a9a1e437ab01725575091cb22
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spelling	oai:doaj.org-article:b5272b6a9a1e437ab01725575091cb222021-11-15T06:08:46ZEvaluation of Anticancer Bioactive Compounds and Cytotoxicity of Kaffir Lime (Citrus hystrix Dc.) Callus Extract Post Preservation2338-94272338-948610.22146/ijp.1062https://doaj.org/article/b5272b6a9a1e437ab01725575091cb222021-06-01T00:00:00Zhttps://jurnal.ugm.ac.id/v3/IJP/article/view/1062https://doaj.org/toc/2338-9427https://doaj.org/toc/2338-9486Previous studies have shown that kaffir lime extract is toxic to breast cancer T47D cells. To increase the bioactive compound's production for traditional cancer medicine raw material, we induce kaffir lime callus in vitro. One strategy to continuously produce kaffir lime callus is by using callus preservation. Our preliminary study used two preservation methods of callus, which are stored in 4 0C with or without alginate encapsulation. However, low temperatures and alginate encapsulation can be stress factors for plants, affecting the production of bioactive compounds and their anticancer ability. This study's objective was to determine the bioactive compounds of callus with and without preservation and their effect on the cytotoxicity against cancer cells. GC-MS detected the bioactive compounds in the extract, and cytotoxicity of callus against breast cancer (T47D), and non-cancer cell (Vero) is tested using MTT method. The results showed that preservation in 4 0C with and without encapsulation caused a change in bioactive compounds' profile. The change mainly precursor compound in callus, after being preserved and recultured, then changes into intermediates or final compounds, indicating the difference in growth phases. The terpenoid compounds detected after preservation are squalene and geranyl linalool. However, Kaffir lime callus extract pre and post preservation are not cytotoxic to both cancer and non-cancer cells. Therefore, the preservation method did not act as an elicitor to callus.Woro Anindito Sri TunjungSudewi FajarinaBeni Hendro PrabowoFrisca DamayantiAsti WidyasariAries Bagus SasongkoAri IndriantoEndang SemiartiLisna HidayatiUniversitas Gadjah Madaarticlekaffir lime callus, preservation, cytotoxicity, bioactive compoundsPharmacy and materia medicaRS1-441ENIndonesian Journal of Pharmacy, Pp 179-192 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	kaffir lime callus, preservation, cytotoxicity, bioactive compounds Pharmacy and materia medica RS1-441
spellingShingle	kaffir lime callus, preservation, cytotoxicity, bioactive compounds Pharmacy and materia medica RS1-441 Woro Anindito Sri Tunjung Sudewi Fajarina Beni Hendro Prabowo Frisca Damayanti Asti Widyasari Aries Bagus Sasongko Ari Indrianto Endang Semiarti Lisna Hidayati Evaluation of Anticancer Bioactive Compounds and Cytotoxicity of Kaffir Lime (Citrus hystrix Dc.) Callus Extract Post Preservation
description	Previous studies have shown that kaffir lime extract is toxic to breast cancer T47D cells. To increase the bioactive compound's production for traditional cancer medicine raw material, we induce kaffir lime callus in vitro. One strategy to continuously produce kaffir lime callus is by using callus preservation. Our preliminary study used two preservation methods of callus, which are stored in 4 0C with or without alginate encapsulation. However, low temperatures and alginate encapsulation can be stress factors for plants, affecting the production of bioactive compounds and their anticancer ability. This study's objective was to determine the bioactive compounds of callus with and without preservation and their effect on the cytotoxicity against cancer cells. GC-MS detected the bioactive compounds in the extract, and cytotoxicity of callus against breast cancer (T47D), and non-cancer cell (Vero) is tested using MTT method. The results showed that preservation in 4 0C with and without encapsulation caused a change in bioactive compounds' profile. The change mainly precursor compound in callus, after being preserved and recultured, then changes into intermediates or final compounds, indicating the difference in growth phases. The terpenoid compounds detected after preservation are squalene and geranyl linalool. However, Kaffir lime callus extract pre and post preservation are not cytotoxic to both cancer and non-cancer cells. Therefore, the preservation method did not act as an elicitor to callus.
format	article
author	Woro Anindito Sri Tunjung Sudewi Fajarina Beni Hendro Prabowo Frisca Damayanti Asti Widyasari Aries Bagus Sasongko Ari Indrianto Endang Semiarti Lisna Hidayati
author_facet	Woro Anindito Sri Tunjung Sudewi Fajarina Beni Hendro Prabowo Frisca Damayanti Asti Widyasari Aries Bagus Sasongko Ari Indrianto Endang Semiarti Lisna Hidayati
author_sort	Woro Anindito Sri Tunjung
title	Evaluation of Anticancer Bioactive Compounds and Cytotoxicity of Kaffir Lime (Citrus hystrix Dc.) Callus Extract Post Preservation
title_short	Evaluation of Anticancer Bioactive Compounds and Cytotoxicity of Kaffir Lime (Citrus hystrix Dc.) Callus Extract Post Preservation
title_full	Evaluation of Anticancer Bioactive Compounds and Cytotoxicity of Kaffir Lime (Citrus hystrix Dc.) Callus Extract Post Preservation
title_fullStr	Evaluation of Anticancer Bioactive Compounds and Cytotoxicity of Kaffir Lime (Citrus hystrix Dc.) Callus Extract Post Preservation
title_full_unstemmed	Evaluation of Anticancer Bioactive Compounds and Cytotoxicity of Kaffir Lime (Citrus hystrix Dc.) Callus Extract Post Preservation
title_sort	evaluation of anticancer bioactive compounds and cytotoxicity of kaffir lime (citrus hystrix dc.) callus extract post preservation
publisher	Universitas Gadjah Mada
publishDate	2021
url	https://doaj.org/article/b5272b6a9a1e437ab01725575091cb22
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Evaluation of Anticancer Bioactive Compounds and Cytotoxicity of Kaffir Lime (Citrus hystrix Dc.) Callus Extract Post Preservation

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