Transcriptome-Wide Map of N6-Methyladenosine Methylome Profiling in Human Bladder Cancer

N6-Methyladenosine (m6A) is the most widespread internal RNA modification in several species. In spite of latest advances in researching the biological roles of m6A, its function in the development and progression of bladder cancer remains unclear. In this study, we used MeRIPty -55-seq and RNA-seq...

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Autores principales:	Aolin Li, Ying Gan, Congcong Cao, Binglei Ma, Quan Zhang, Qian Zhang, Lin Yao
Formato:	article
Lenguaje:	EN
Publicado:	Frontiers Media S.A. 2021
Materias:	m6A (N6-methyladenosine) MeRIP-seq bladder cancer mRNA lncRNA circRNA Neoplasms. Tumors. Oncology. Including cancer and carcinogens RC254-282
Acceso en línea:	https://doaj.org/article/b527f069bfac43d29b6180bdc7f219be
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spelling	oai:doaj.org-article:b527f069bfac43d29b6180bdc7f219be2021-11-15T05:33:38ZTranscriptome-Wide Map of N6-Methyladenosine Methylome Profiling in Human Bladder Cancer2234-943X10.3389/fonc.2021.717622https://doaj.org/article/b527f069bfac43d29b6180bdc7f219be2021-11-01T00:00:00Zhttps://www.frontiersin.org/articles/10.3389/fonc.2021.717622/fullhttps://doaj.org/toc/2234-943XN6-Methyladenosine (m6A) is the most widespread internal RNA modification in several species. In spite of latest advances in researching the biological roles of m6A, its function in the development and progression of bladder cancer remains unclear. In this study, we used MeRIPty -55-seq and RNA-seq methods to obtain a comprehensive transcriptome-wide m6A profiling and gene expression pattern in bladder cancer and paired normal adjacent tissues. Our findings showed that there were 2,331 hypomethylated and 3,819 hypermethylated mRNAs, 32 hypomethylated and 105 hypermethylated lncRNAs, and 15 hypomethylated and 238 hypermethylated circRNAs in bladder cancer tissues compared to adjacent normal tissues. Furthermore, m6A is most often harbored in the coding sequence (CDS), with some near the start and stop codons between two groups. Functional enrichment analysis revealed that differentially methylated mRNAs, lncRNAs, and circRNAs were mostly enriched in transcriptional misregulation in cancer and TNF signaling pathway. We also found that different m6A methylation levels of gene might regulate its expression. In summary, our results for the first time provide an m6A landscape of human bladder cancer, which expand the understanding of m6A modifications and uncover the regulation of mRNAs, lncRNAs, and circRNAs through m6A modification in bladder cancer.Aolin LiAolin LiAolin LiAolin LiYing GanYing GanYing GanYing GanCongcong CaoBinglei MaBinglei MaBinglei MaBinglei MaQuan ZhangQuan ZhangQuan ZhangQuan ZhangQian ZhangQian ZhangQian ZhangQian ZhangLin YaoLin YaoLin YaoLin YaoFrontiers Media S.A.articlem6A (N6-methyladenosine)MeRIP-seqbladder cancermRNAlncRNAcircRNANeoplasms. Tumors. Oncology. Including cancer and carcinogensRC254-282ENFrontiers in Oncology, Vol 11 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	m6A (N6-methyladenosine) MeRIP-seq bladder cancer mRNA lncRNA circRNA Neoplasms. Tumors. Oncology. Including cancer and carcinogens RC254-282
spellingShingle	m6A (N6-methyladenosine) MeRIP-seq bladder cancer mRNA lncRNA circRNA Neoplasms. Tumors. Oncology. Including cancer and carcinogens RC254-282 Aolin Li Aolin Li Aolin Li Aolin Li Ying Gan Ying Gan Ying Gan Ying Gan Congcong Cao Binglei Ma Binglei Ma Binglei Ma Binglei Ma Quan Zhang Quan Zhang Quan Zhang Quan Zhang Qian Zhang Qian Zhang Qian Zhang Qian Zhang Lin Yao Lin Yao Lin Yao Lin Yao Transcriptome-Wide Map of N6-Methyladenosine Methylome Profiling in Human Bladder Cancer
description	N6-Methyladenosine (m6A) is the most widespread internal RNA modification in several species. In spite of latest advances in researching the biological roles of m6A, its function in the development and progression of bladder cancer remains unclear. In this study, we used MeRIPty -55-seq and RNA-seq methods to obtain a comprehensive transcriptome-wide m6A profiling and gene expression pattern in bladder cancer and paired normal adjacent tissues. Our findings showed that there were 2,331 hypomethylated and 3,819 hypermethylated mRNAs, 32 hypomethylated and 105 hypermethylated lncRNAs, and 15 hypomethylated and 238 hypermethylated circRNAs in bladder cancer tissues compared to adjacent normal tissues. Furthermore, m6A is most often harbored in the coding sequence (CDS), with some near the start and stop codons between two groups. Functional enrichment analysis revealed that differentially methylated mRNAs, lncRNAs, and circRNAs were mostly enriched in transcriptional misregulation in cancer and TNF signaling pathway. We also found that different m6A methylation levels of gene might regulate its expression. In summary, our results for the first time provide an m6A landscape of human bladder cancer, which expand the understanding of m6A modifications and uncover the regulation of mRNAs, lncRNAs, and circRNAs through m6A modification in bladder cancer.
format	article
author	Aolin Li Aolin Li Aolin Li Aolin Li Ying Gan Ying Gan Ying Gan Ying Gan Congcong Cao Binglei Ma Binglei Ma Binglei Ma Binglei Ma Quan Zhang Quan Zhang Quan Zhang Quan Zhang Qian Zhang Qian Zhang Qian Zhang Qian Zhang Lin Yao Lin Yao Lin Yao Lin Yao
author_facet	Aolin Li Aolin Li Aolin Li Aolin Li Ying Gan Ying Gan Ying Gan Ying Gan Congcong Cao Binglei Ma Binglei Ma Binglei Ma Binglei Ma Quan Zhang Quan Zhang Quan Zhang Quan Zhang Qian Zhang Qian Zhang Qian Zhang Qian Zhang Lin Yao Lin Yao Lin Yao Lin Yao
author_sort	Aolin Li
title	Transcriptome-Wide Map of N6-Methyladenosine Methylome Profiling in Human Bladder Cancer
title_short	Transcriptome-Wide Map of N6-Methyladenosine Methylome Profiling in Human Bladder Cancer
title_full	Transcriptome-Wide Map of N6-Methyladenosine Methylome Profiling in Human Bladder Cancer
title_fullStr	Transcriptome-Wide Map of N6-Methyladenosine Methylome Profiling in Human Bladder Cancer
title_full_unstemmed	Transcriptome-Wide Map of N6-Methyladenosine Methylome Profiling in Human Bladder Cancer
title_sort	transcriptome-wide map of n6-methyladenosine methylome profiling in human bladder cancer
publisher	Frontiers Media S.A.
publishDate	2021
url	https://doaj.org/article/b527f069bfac43d29b6180bdc7f219be
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Transcriptome-Wide Map of N6-Methyladenosine Methylome Profiling in Human Bladder Cancer

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