Fibroin and sericin from Bombyx mori silk stimulate cell migration through upregulation and phosphorylation of c-Jun.

Wound healing is a biological process directed to the restoration of tissue that has suffered an injury. An important phase of wound healing is the generation of a basal epithelium able to wholly replace the epidermis of the wound. A broad range of products derived from fibroin and sericin from Bomb...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Celia Martínez-Mora, Anna Mrowiec, Eva María García-Vizcaíno, Antonia Alcaraz, José Luis Cenis, Francisco José Nicolás
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2012
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/b530bd87636d4b2eb98669ebc01af49d
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:b530bd87636d4b2eb98669ebc01af49d
record_format	dspace
spelling	oai:doaj.org-article:b530bd87636d4b2eb98669ebc01af49d2021-11-18T07:10:13ZFibroin and sericin from Bombyx mori silk stimulate cell migration through upregulation and phosphorylation of c-Jun.1932-620310.1371/journal.pone.0042271https://doaj.org/article/b530bd87636d4b2eb98669ebc01af49d2012-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/22860103/pdf/?tool=EBIhttps://doaj.org/toc/1932-6203Wound healing is a biological process directed to the restoration of tissue that has suffered an injury. An important phase of wound healing is the generation of a basal epithelium able to wholly replace the epidermis of the wound. A broad range of products derived from fibroin and sericin from Bombyx mori silk are used to stimulate wound healing. However, so far the molecular mechanism underlying this phenomenon has not been elucidated. The aim of this work was to determine the molecular basis underlying wound healing properties of silk proteins using a cell model. For this purpose, we assayed fibroin and sericin in a wound healing scratch assay using MDA-MB-231 and Mv1Lu cells. Both proteins stimulated cell migration. Furthermore, treatment with sericin and fibroin involved key factors of the wound healing process such as upregulation of c-Jun and c-Jun protein phosphorylation. Moreover, fibroin and sericin stimulated the phosphorylation of ERK 1/2 and JNK 1/2 kinases. All these experiments were done in the presence of specific inhibitors for some of the cell signalling pathways referred above. The obtained results revealed that MEK, JNK and PI3K pathways are involved in fibroin and sericin stimulated cells migration. Inhibition of these three kinases prevented c-Jun upregulation and phosphorylation by fibroin or sericin. Fibroin and sericin were tested in the human keratinocyte cell line, HaCaT, with similar results. Altogether, our results showed that fibroin and sericin initiate cell migration by activating the MEK, JNK and PI3K signalling pathways ending in c-Jun activation.Celia Martínez-MoraAnna MrowiecEva María García-VizcaínoAntonia AlcarazJosé Luis CenisFrancisco José NicolásPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 7, Iss 7, p e42271 (2012)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Celia Martínez-Mora Anna Mrowiec Eva María García-Vizcaíno Antonia Alcaraz José Luis Cenis Francisco José Nicolás Fibroin and sericin from Bombyx mori silk stimulate cell migration through upregulation and phosphorylation of c-Jun.
description	Wound healing is a biological process directed to the restoration of tissue that has suffered an injury. An important phase of wound healing is the generation of a basal epithelium able to wholly replace the epidermis of the wound. A broad range of products derived from fibroin and sericin from Bombyx mori silk are used to stimulate wound healing. However, so far the molecular mechanism underlying this phenomenon has not been elucidated. The aim of this work was to determine the molecular basis underlying wound healing properties of silk proteins using a cell model. For this purpose, we assayed fibroin and sericin in a wound healing scratch assay using MDA-MB-231 and Mv1Lu cells. Both proteins stimulated cell migration. Furthermore, treatment with sericin and fibroin involved key factors of the wound healing process such as upregulation of c-Jun and c-Jun protein phosphorylation. Moreover, fibroin and sericin stimulated the phosphorylation of ERK 1/2 and JNK 1/2 kinases. All these experiments were done in the presence of specific inhibitors for some of the cell signalling pathways referred above. The obtained results revealed that MEK, JNK and PI3K pathways are involved in fibroin and sericin stimulated cells migration. Inhibition of these three kinases prevented c-Jun upregulation and phosphorylation by fibroin or sericin. Fibroin and sericin were tested in the human keratinocyte cell line, HaCaT, with similar results. Altogether, our results showed that fibroin and sericin initiate cell migration by activating the MEK, JNK and PI3K signalling pathways ending in c-Jun activation.
format	article
author	Celia Martínez-Mora Anna Mrowiec Eva María García-Vizcaíno Antonia Alcaraz José Luis Cenis Francisco José Nicolás
author_facet	Celia Martínez-Mora Anna Mrowiec Eva María García-Vizcaíno Antonia Alcaraz José Luis Cenis Francisco José Nicolás
author_sort	Celia Martínez-Mora
title	Fibroin and sericin from Bombyx mori silk stimulate cell migration through upregulation and phosphorylation of c-Jun.
title_short	Fibroin and sericin from Bombyx mori silk stimulate cell migration through upregulation and phosphorylation of c-Jun.
title_full	Fibroin and sericin from Bombyx mori silk stimulate cell migration through upregulation and phosphorylation of c-Jun.
title_fullStr	Fibroin and sericin from Bombyx mori silk stimulate cell migration through upregulation and phosphorylation of c-Jun.
title_full_unstemmed	Fibroin and sericin from Bombyx mori silk stimulate cell migration through upregulation and phosphorylation of c-Jun.
title_sort	fibroin and sericin from bombyx mori silk stimulate cell migration through upregulation and phosphorylation of c-jun.
publisher	Public Library of Science (PLoS)
publishDate	2012
url	https://doaj.org/article/b530bd87636d4b2eb98669ebc01af49d
work_keys_str_mv	AT celiamartinezmora fibroinandsericinfrombombyxmorisilkstimulatecellmigrationthroughupregulationandphosphorylationofcjun AT annamrowiec fibroinandsericinfrombombyxmorisilkstimulatecellmigrationthroughupregulationandphosphorylationofcjun AT evamariagarciavizcaino fibroinandsericinfrombombyxmorisilkstimulatecellmigrationthroughupregulationandphosphorylationofcjun AT antoniaalcaraz fibroinandsericinfrombombyxmorisilkstimulatecellmigrationthroughupregulationandphosphorylationofcjun AT joseluiscenis fibroinandsericinfrombombyxmorisilkstimulatecellmigrationthroughupregulationandphosphorylationofcjun AT franciscojosenicolas fibroinandsericinfrombombyxmorisilkstimulatecellmigrationthroughupregulationandphosphorylationofcjun
_version_	1718423876188241920

Fibroin and sericin from Bombyx mori silk stimulate cell migration through upregulation and phosphorylation of c-Jun.

Ejemplares similares