Hypertrophic stimulation increases beta-actin dynamics in adult feline cardiomyocytes.

The myocardium responds to hemodynamic stress through cellular growth and organ hypertrophy. The impact of cytoskeletal elements on this process, however, is not fully understood. While alpha-actin in cardiomyocytes governs muscle contraction in combination with the myosin motor, the exact role of b...

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Autores principales:	Sundaravadivel Balasubramanian, Santhosh K Mani, Harinath Kasiganesan, Catalin C Baicu, Dhandapani Kuppuswamy
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2010
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/b533146291134fb7b6a47d7e3a70d053
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spelling	oai:doaj.org-article:b533146291134fb7b6a47d7e3a70d0532021-12-02T20:20:08ZHypertrophic stimulation increases beta-actin dynamics in adult feline cardiomyocytes.1932-620310.1371/journal.pone.0011470https://doaj.org/article/b533146291134fb7b6a47d7e3a70d0532010-07-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/20635003/?tool=EBIhttps://doaj.org/toc/1932-6203The myocardium responds to hemodynamic stress through cellular growth and organ hypertrophy. The impact of cytoskeletal elements on this process, however, is not fully understood. While alpha-actin in cardiomyocytes governs muscle contraction in combination with the myosin motor, the exact role of beta-actin has not been established. We hypothesized that in adult cardiomyocytes, as in non-myocytes, beta-actin can facilitate cytoskeletal rearrangement within cytoskeletal structures such as Z-discs. Using a feline right ventricular pressure overload (RVPO) model, we measured the level and distribution of beta-actin in normal and pressure overloaded myocardium. Resulting data demonstrated enriched levels of beta-actin and enhanced translocation to the Triton-insoluble cytoskeletal and membrane skeletal complexes. In addition, RVPO in vivo and in vitro hypertrophic stimulation with endothelin (ET) or insulin in isolated adult cardiomyocytes enhanced the content of polymerized fraction (F-actin) of beta-actin. To determine the localization and dynamics of beta-actin, we adenovirally expressed GFP-tagged beta-actin in isolated adult cardiomyocytes. The ectopically expressed beta-actin-GFP localized to the Z-discs, costameres, and cell termini. Fluorescence recovery after photobleaching (FRAP) measurements of beta-actin dynamics revealed that beta-actin at the Z-discs is constantly being exchanged with beta-actin from cytoplasmic pools and that this exchange is faster upon hypertrophic stimulation with ET or insulin. In addition, in electrically stimulated isolated adult cardiomyocytes, while beta-actin overexpression improved cardiomyocyte contractility, immunoneutralization of beta-actin resulted in a reduced contractility suggesting that beta-actin could be important for the contractile function of adult cardiomyocytes. These studies demonstrate the presence and dynamics of beta-actin in the adult cardiomyocyte and reinforce its usefulness in measuring cardiac cytoskeletal rearrangement during hypertrophic stimulation.Sundaravadivel BalasubramanianSanthosh K ManiHarinath KasiganesanCatalin C BaicuDhandapani KuppuswamyPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 5, Iss 7, p e11470 (2010)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Sundaravadivel Balasubramanian Santhosh K Mani Harinath Kasiganesan Catalin C Baicu Dhandapani Kuppuswamy Hypertrophic stimulation increases beta-actin dynamics in adult feline cardiomyocytes.
description	The myocardium responds to hemodynamic stress through cellular growth and organ hypertrophy. The impact of cytoskeletal elements on this process, however, is not fully understood. While alpha-actin in cardiomyocytes governs muscle contraction in combination with the myosin motor, the exact role of beta-actin has not been established. We hypothesized that in adult cardiomyocytes, as in non-myocytes, beta-actin can facilitate cytoskeletal rearrangement within cytoskeletal structures such as Z-discs. Using a feline right ventricular pressure overload (RVPO) model, we measured the level and distribution of beta-actin in normal and pressure overloaded myocardium. Resulting data demonstrated enriched levels of beta-actin and enhanced translocation to the Triton-insoluble cytoskeletal and membrane skeletal complexes. In addition, RVPO in vivo and in vitro hypertrophic stimulation with endothelin (ET) or insulin in isolated adult cardiomyocytes enhanced the content of polymerized fraction (F-actin) of beta-actin. To determine the localization and dynamics of beta-actin, we adenovirally expressed GFP-tagged beta-actin in isolated adult cardiomyocytes. The ectopically expressed beta-actin-GFP localized to the Z-discs, costameres, and cell termini. Fluorescence recovery after photobleaching (FRAP) measurements of beta-actin dynamics revealed that beta-actin at the Z-discs is constantly being exchanged with beta-actin from cytoplasmic pools and that this exchange is faster upon hypertrophic stimulation with ET or insulin. In addition, in electrically stimulated isolated adult cardiomyocytes, while beta-actin overexpression improved cardiomyocyte contractility, immunoneutralization of beta-actin resulted in a reduced contractility suggesting that beta-actin could be important for the contractile function of adult cardiomyocytes. These studies demonstrate the presence and dynamics of beta-actin in the adult cardiomyocyte and reinforce its usefulness in measuring cardiac cytoskeletal rearrangement during hypertrophic stimulation.
format	article
author	Sundaravadivel Balasubramanian Santhosh K Mani Harinath Kasiganesan Catalin C Baicu Dhandapani Kuppuswamy
author_facet	Sundaravadivel Balasubramanian Santhosh K Mani Harinath Kasiganesan Catalin C Baicu Dhandapani Kuppuswamy
author_sort	Sundaravadivel Balasubramanian
title	Hypertrophic stimulation increases beta-actin dynamics in adult feline cardiomyocytes.
title_short	Hypertrophic stimulation increases beta-actin dynamics in adult feline cardiomyocytes.
title_full	Hypertrophic stimulation increases beta-actin dynamics in adult feline cardiomyocytes.
title_fullStr	Hypertrophic stimulation increases beta-actin dynamics in adult feline cardiomyocytes.
title_full_unstemmed	Hypertrophic stimulation increases beta-actin dynamics in adult feline cardiomyocytes.
title_sort	hypertrophic stimulation increases beta-actin dynamics in adult feline cardiomyocytes.
publisher	Public Library of Science (PLoS)
publishDate	2010
url	https://doaj.org/article/b533146291134fb7b6a47d7e3a70d053
work_keys_str_mv	AT sundaravadivelbalasubramanian hypertrophicstimulationincreasesbetaactindynamicsinadultfelinecardiomyocytes AT santhoshkmani hypertrophicstimulationincreasesbetaactindynamicsinadultfelinecardiomyocytes AT harinathkasiganesan hypertrophicstimulationincreasesbetaactindynamicsinadultfelinecardiomyocytes AT catalincbaicu hypertrophicstimulationincreasesbetaactindynamicsinadultfelinecardiomyocytes AT dhandapanikuppuswamy hypertrophicstimulationincreasesbetaactindynamicsinadultfelinecardiomyocytes
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Hypertrophic stimulation increases beta-actin dynamics in adult feline cardiomyocytes.

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