Enhanced photodynamic leishmanicidal activity of hydrophobic zinc phthalocyanine within archaeolipids containing liposomes

Ana Paula Perez,1 Agustina Casasco,2 Priscila Schilrreff,1 Maria Victoria Defain Tesoriero,1,3 Luc Duempelmann,1 Maria Julia Altube,1 Leticia Higa,1 Maria Jose Morilla,1 Patricia Petray,2 Eder L Romero11Programa de Nanomedicinas, Departamento de Ciencia y Tecnología, Universidad Nacional...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Perez AP, Casasco A, Schilrreff P, Defain Tesoriero MV, Duempelmann L, Altube MJ, Higa L, Morilla MJ, Petray P, Romero EL
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2014
Materias:	Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/b5413c4d9d2b49e9a7da02cc3ad92883
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:b5413c4d9d2b49e9a7da02cc3ad92883
record_format	dspace
spelling	oai:doaj.org-article:b5413c4d9d2b49e9a7da02cc3ad928832021-12-02T03:58:30ZEnhanced photodynamic leishmanicidal activity of hydrophobic zinc phthalocyanine within archaeolipids containing liposomes1178-2013https://doaj.org/article/b5413c4d9d2b49e9a7da02cc3ad928832014-07-01T00:00:00Zhttp://www.dovepress.com/enhanced-photodynamic-leishmanicidal-activity-of-hydrophobic-zinc-phth-a17548https://doaj.org/toc/1178-2013 Ana Paula Perez,1 Agustina Casasco,2 Priscila Schilrreff,1 Maria Victoria Defain Tesoriero,1,3 Luc Duempelmann,1 Maria Julia Altube,1 Leticia Higa,1 Maria Jose Morilla,1 Patricia Petray,2 Eder L Romero11Programa de Nanomedicinas, Departamento de Ciencia y Tecnología, Universidad Nacional de Quilmes, 2Servicio de Parasitología y Enfermedad de Chagas, Hospital de Niños Ricardo Gutiérrez, 3Unidad Operativa Sistemas de Liberación Controlada, Centro de Investigación y Desarrollo en Química, Instituto Nacional de Tecnología Industrial (INTI), Buenos Aires, ArgentinaAbstract: In this work, the in vitro anti-Leishmania activity of photodynamic liposomes made of soybean phosphatidylcholine, sodium cholate, total polar archaeolipids (TPAs) extracted from the hyperhalophile archaea Halorubrum tebenquichense and the photosensitizer zinc phthalocyanine (ZnPcAL) was compared to that of ultradeformable photodynamic liposomes lacking TPAs (ZnPcUDLs). We found that while ZnPcUDLs and ZnPcALs (130 nm mean diameter and –35 mV zeta potential) were innocuous against promastigotes, a low concentration (0.01 µM ZnPc and 7.6 µM phospholipids) of ZnPcALs irradiated at a very low-energy density (0.2 J/cm2) eliminated L. braziliensis amastigotes from J774 macrophages, without reducing the viability of the host cells. In such conditions, ZnPcALs were harmless for J774 macrophages, HaCaT keratinocytes, and bone marrow-derived dendritic cells. Therefore, topical photodynamic treatment would not likely affect skin-associated lymphoid tissue. ZnPcALs were extensively captured by macrophages, but ZnPcUDLs were not, leading to 2.5-fold increased intracellular delivery of ZnPc than with ZnPcUDLs. Despite mediating low levels of reactive oxygen species, the higher delivery of ZnPc and the multiple (caveolin- and clathrin-dependent plus phagocytic) intracellular pathway followed by ZnPc would have been the reason for the higher antiamastigote activity of ZnPcALs. The leishmanicidal activity of photodynamic liposomal ZnPc was improved by TPA-containing liposomes.Keywords: macrophages, Leishmania amastigotes, Zn intracellular delivery, irradiationPerez APCasasco ASchilrreff PDefain Tesoriero MVDuempelmann LAltube MJHiga LMorilla MJPetray PRomero ELDove Medical PressarticleMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol 2014, Iss Issue 1, Pp 3335-3345 (2014)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine (General) R5-920
spellingShingle	Medicine (General) R5-920 Perez AP Casasco A Schilrreff P Defain Tesoriero MV Duempelmann L Altube MJ Higa L Morilla MJ Petray P Romero EL Enhanced photodynamic leishmanicidal activity of hydrophobic zinc phthalocyanine within archaeolipids containing liposomes
description	Ana Paula Perez,1 Agustina Casasco,2 Priscila Schilrreff,1 Maria Victoria Defain Tesoriero,1,3 Luc Duempelmann,1 Maria Julia Altube,1 Leticia Higa,1 Maria Jose Morilla,1 Patricia Petray,2 Eder L Romero11Programa de Nanomedicinas, Departamento de Ciencia y Tecnología, Universidad Nacional de Quilmes, 2Servicio de Parasitología y Enfermedad de Chagas, Hospital de Niños Ricardo Gutiérrez, 3Unidad Operativa Sistemas de Liberación Controlada, Centro de Investigación y Desarrollo en Química, Instituto Nacional de Tecnología Industrial (INTI), Buenos Aires, ArgentinaAbstract: In this work, the in vitro anti-Leishmania activity of photodynamic liposomes made of soybean phosphatidylcholine, sodium cholate, total polar archaeolipids (TPAs) extracted from the hyperhalophile archaea Halorubrum tebenquichense and the photosensitizer zinc phthalocyanine (ZnPcAL) was compared to that of ultradeformable photodynamic liposomes lacking TPAs (ZnPcUDLs). We found that while ZnPcUDLs and ZnPcALs (130 nm mean diameter and –35 mV zeta potential) were innocuous against promastigotes, a low concentration (0.01 µM ZnPc and 7.6 µM phospholipids) of ZnPcALs irradiated at a very low-energy density (0.2 J/cm2) eliminated L. braziliensis amastigotes from J774 macrophages, without reducing the viability of the host cells. In such conditions, ZnPcALs were harmless for J774 macrophages, HaCaT keratinocytes, and bone marrow-derived dendritic cells. Therefore, topical photodynamic treatment would not likely affect skin-associated lymphoid tissue. ZnPcALs were extensively captured by macrophages, but ZnPcUDLs were not, leading to 2.5-fold increased intracellular delivery of ZnPc than with ZnPcUDLs. Despite mediating low levels of reactive oxygen species, the higher delivery of ZnPc and the multiple (caveolin- and clathrin-dependent plus phagocytic) intracellular pathway followed by ZnPc would have been the reason for the higher antiamastigote activity of ZnPcALs. The leishmanicidal activity of photodynamic liposomal ZnPc was improved by TPA-containing liposomes.Keywords: macrophages, Leishmania amastigotes, Zn intracellular delivery, irradiation
format	article
author	Perez AP Casasco A Schilrreff P Defain Tesoriero MV Duempelmann L Altube MJ Higa L Morilla MJ Petray P Romero EL
author_facet	Perez AP Casasco A Schilrreff P Defain Tesoriero MV Duempelmann L Altube MJ Higa L Morilla MJ Petray P Romero EL
author_sort	Perez AP
title	Enhanced photodynamic leishmanicidal activity of hydrophobic zinc phthalocyanine within archaeolipids containing liposomes
title_short	Enhanced photodynamic leishmanicidal activity of hydrophobic zinc phthalocyanine within archaeolipids containing liposomes
title_full	Enhanced photodynamic leishmanicidal activity of hydrophobic zinc phthalocyanine within archaeolipids containing liposomes
title_fullStr	Enhanced photodynamic leishmanicidal activity of hydrophobic zinc phthalocyanine within archaeolipids containing liposomes
title_full_unstemmed	Enhanced photodynamic leishmanicidal activity of hydrophobic zinc phthalocyanine within archaeolipids containing liposomes
title_sort	enhanced photodynamic leishmanicidal activity of hydrophobic zinc phthalocyanine within archaeolipids containing liposomes
publisher	Dove Medical Press
publishDate	2014
url	https://doaj.org/article/b5413c4d9d2b49e9a7da02cc3ad92883
work_keys_str_mv	AT perezap enhancedphotodynamicleishmanicidalactivityofhydrophobiczincphthalocyaninewithinarchaeolipidscontainingliposomes AT casascoa enhancedphotodynamicleishmanicidalactivityofhydrophobiczincphthalocyaninewithinarchaeolipidscontainingliposomes AT schilrreffp enhancedphotodynamicleishmanicidalactivityofhydrophobiczincphthalocyaninewithinarchaeolipidscontainingliposomes AT defaintesorieromv enhancedphotodynamicleishmanicidalactivityofhydrophobiczincphthalocyaninewithinarchaeolipidscontainingliposomes AT duempelmannl enhancedphotodynamicleishmanicidalactivityofhydrophobiczincphthalocyaninewithinarchaeolipidscontainingliposomes AT altubemj enhancedphotodynamicleishmanicidalactivityofhydrophobiczincphthalocyaninewithinarchaeolipidscontainingliposomes AT higal enhancedphotodynamicleishmanicidalactivityofhydrophobiczincphthalocyaninewithinarchaeolipidscontainingliposomes AT morillamj enhancedphotodynamicleishmanicidalactivityofhydrophobiczincphthalocyaninewithinarchaeolipidscontainingliposomes AT petrayp enhancedphotodynamicleishmanicidalactivityofhydrophobiczincphthalocyaninewithinarchaeolipidscontainingliposomes AT romeroel enhancedphotodynamicleishmanicidalactivityofhydrophobiczincphthalocyaninewithinarchaeolipidscontainingliposomes
_version_	1718401511808040960

Enhanced photodynamic leishmanicidal activity of hydrophobic zinc phthalocyanine within archaeolipids containing liposomes

Ejemplares similares