Mucin secretory action of capsaicin prevents high fat diet-induced gut barrier dysfunction in C57BL/6 mice colon

The gut barrier – including tight junction proteins (TJPs) and mucus layers, is the first line of defense against physical, chemical or pathogenic incursions. This barrier is compromised in various health disorders. Capsaicin, a dietary agonist of Transient receptor potential vanilloid 1 (TRPV1) cha...

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Autores principales:	Vijay Kumar, Vibhu Kumar, Neha Mahajan, Jasleen Kaur, Kirti Devi, Ravinder Naik Dharavath, Ravindra Pal Singh, Kanthi Kiran Kondepudi, Mahendra Bishnoi
Formato:	article
Lenguaje:	EN
Publicado:	Elsevier 2022
Materias:	Capsaicin Gut mucus Gut bacteria TRPV1 Obesity Therapeutics. Pharmacology RM1-950
Acceso en línea:	https://doaj.org/article/b54f5e11c28f47e2833718e204bb5750
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spelling	oai:doaj.org-article:b54f5e11c28f47e2833718e204bb57502021-11-22T04:17:57ZMucin secretory action of capsaicin prevents high fat diet-induced gut barrier dysfunction in C57BL/6 mice colon0753-332210.1016/j.biopha.2021.112452https://doaj.org/article/b54f5e11c28f47e2833718e204bb57502022-01-01T00:00:00Zhttp://www.sciencedirect.com/science/article/pii/S0753332221012385https://doaj.org/toc/0753-3322The gut barrier – including tight junction proteins (TJPs) and mucus layers, is the first line of defense against physical, chemical or pathogenic incursions. This barrier is compromised in various health disorders. Capsaicin, a dietary agonist of Transient receptor potential vanilloid 1 (TRPV1) channel, is reported to alleviate the complications of obesity. While it is well known to improve energy expenditure and metabolism, and prevent dysbiosis, the more local effects on the host gut – particularly the gut barrier and mucus system remain elusive. To investigate the effect of capsaicin on the gut barrier and mucus production and to understand the involvement of mucus, bacteria, and TRPV1 in these phenomena, we employed a diet-induced obesity model in C57BL/6 mice, and capsaicin (2 mg/kg/day p.o.) or mucin (1 g/kg/day p.o.) as interventions, for 12 weeks. Parameters like weight gain, glucose homeostasis, TJPs expression, mucus staining, intestinal permeability etc were studied. 16 S rDNA sequencing and in vitro Ca2+ measurement experiments were performed to explore the role of microbiota in the beneficial effects. Mucin feeding reflected several anti-obesity effects produced by capsaicin, suggesting that mucus modulation might play a crucial role in capsaicin-induced anti-obesity effects. 16 S rDNA sequencing and in vitro Ca2+ measurement experiments pointed to TRPV1 modulation by bacteria besides capsaicin. Capsaicin, bacteria and the host mucus system seem to act in a cyclic cascade involving TRPV1, which can be activated by capsaicin and various bacteria. These findings provide new insight into the role of TRPV1 in maintaining a healthy gut environment.Vijay KumarVibhu KumarNeha MahajanJasleen KaurKirti DeviRavinder Naik DharavathRavindra Pal SinghKanthi Kiran KondepudiMahendra BishnoiElsevierarticleCapsaicinGut mucusGut bacteriaTRPV1ObesityTherapeutics. PharmacologyRM1-950ENBiomedicine & Pharmacotherapy, Vol 145, Iss , Pp 112452- (2022)
institution	DOAJ
collection	DOAJ
language	EN
topic	Capsaicin Gut mucus Gut bacteria TRPV1 Obesity Therapeutics. Pharmacology RM1-950
spellingShingle	Capsaicin Gut mucus Gut bacteria TRPV1 Obesity Therapeutics. Pharmacology RM1-950 Vijay Kumar Vibhu Kumar Neha Mahajan Jasleen Kaur Kirti Devi Ravinder Naik Dharavath Ravindra Pal Singh Kanthi Kiran Kondepudi Mahendra Bishnoi Mucin secretory action of capsaicin prevents high fat diet-induced gut barrier dysfunction in C57BL/6 mice colon
description	The gut barrier – including tight junction proteins (TJPs) and mucus layers, is the first line of defense against physical, chemical or pathogenic incursions. This barrier is compromised in various health disorders. Capsaicin, a dietary agonist of Transient receptor potential vanilloid 1 (TRPV1) channel, is reported to alleviate the complications of obesity. While it is well known to improve energy expenditure and metabolism, and prevent dysbiosis, the more local effects on the host gut – particularly the gut barrier and mucus system remain elusive. To investigate the effect of capsaicin on the gut barrier and mucus production and to understand the involvement of mucus, bacteria, and TRPV1 in these phenomena, we employed a diet-induced obesity model in C57BL/6 mice, and capsaicin (2 mg/kg/day p.o.) or mucin (1 g/kg/day p.o.) as interventions, for 12 weeks. Parameters like weight gain, glucose homeostasis, TJPs expression, mucus staining, intestinal permeability etc were studied. 16 S rDNA sequencing and in vitro Ca2+ measurement experiments were performed to explore the role of microbiota in the beneficial effects. Mucin feeding reflected several anti-obesity effects produced by capsaicin, suggesting that mucus modulation might play a crucial role in capsaicin-induced anti-obesity effects. 16 S rDNA sequencing and in vitro Ca2+ measurement experiments pointed to TRPV1 modulation by bacteria besides capsaicin. Capsaicin, bacteria and the host mucus system seem to act in a cyclic cascade involving TRPV1, which can be activated by capsaicin and various bacteria. These findings provide new insight into the role of TRPV1 in maintaining a healthy gut environment.
format	article
author	Vijay Kumar Vibhu Kumar Neha Mahajan Jasleen Kaur Kirti Devi Ravinder Naik Dharavath Ravindra Pal Singh Kanthi Kiran Kondepudi Mahendra Bishnoi
author_facet	Vijay Kumar Vibhu Kumar Neha Mahajan Jasleen Kaur Kirti Devi Ravinder Naik Dharavath Ravindra Pal Singh Kanthi Kiran Kondepudi Mahendra Bishnoi
author_sort	Vijay Kumar
title	Mucin secretory action of capsaicin prevents high fat diet-induced gut barrier dysfunction in C57BL/6 mice colon
title_short	Mucin secretory action of capsaicin prevents high fat diet-induced gut barrier dysfunction in C57BL/6 mice colon
title_full	Mucin secretory action of capsaicin prevents high fat diet-induced gut barrier dysfunction in C57BL/6 mice colon
title_fullStr	Mucin secretory action of capsaicin prevents high fat diet-induced gut barrier dysfunction in C57BL/6 mice colon
title_full_unstemmed	Mucin secretory action of capsaicin prevents high fat diet-induced gut barrier dysfunction in C57BL/6 mice colon
title_sort	mucin secretory action of capsaicin prevents high fat diet-induced gut barrier dysfunction in c57bl/6 mice colon
publisher	Elsevier
publishDate	2022
url	https://doaj.org/article/b54f5e11c28f47e2833718e204bb5750
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Mucin secretory action of capsaicin prevents high fat diet-induced gut barrier dysfunction in C57BL/6 mice colon

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