A Non-Rogue Mutant Line Induced by ENU Mutagenesis in Paramutated Rogue Peas (<i>Pisum sativum</i> L.) Is Still Sensitive to the Rogue Paramutation

The spontaneously emerging rogue phenotype in peas (<i>Pisum sativum</i> L.), characterized by narrow and pointed leaf stipula and leaflets, was the first identified case of the epigenetic phenomenon paramutation. The crosses of homozygous or heterozygous (e.g., F1) rogue plants with non...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Ricardo Pereira, José M. Leitão
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	pea Pisum paramutation rogue phenotype 1-ethyl-1-nitrosourea ENU Genetics QH426-470
Acceso en línea:	https://doaj.org/article/b5509d96a1f648488178dc2c5cf01673
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:b5509d96a1f648488178dc2c5cf01673
record_format	dspace
spelling	oai:doaj.org-article:b5509d96a1f648488178dc2c5cf016732021-11-25T17:40:44ZA Non-Rogue Mutant Line Induced by ENU Mutagenesis in Paramutated Rogue Peas (<i>Pisum sativum</i> L.) Is Still Sensitive to the Rogue Paramutation10.3390/genes121116802073-4425https://doaj.org/article/b5509d96a1f648488178dc2c5cf016732021-10-01T00:00:00Zhttps://www.mdpi.com/2073-4425/12/11/1680https://doaj.org/toc/2073-4425The spontaneously emerging rogue phenotype in peas (<i>Pisum sativum</i> L.), characterized by narrow and pointed leaf stipula and leaflets, was the first identified case of the epigenetic phenomenon paramutation. The crosses of homozygous or heterozygous (e.g., F1) rogue plants with non-rogue (wild type) plants, produce exclusively rogue plants in the first and all subsequent generations. The fact that the wild phenotype disappears forever, is in clear contradiction with the Mendelian rules of inheritance, a situation that impedes the positional cloning of genes involved in this epigenetic phenomenon. One way of overcoming this obstacle is the identification of plant genotypes harboring naturally occurring or artificially induced neutral alleles, non-sensitive to paramutation. So far, such alleles have never been described for the pea rogue paramutation. Here, we report the induction via 1-ethyl-1-nitrosourea (ENU) mutagenesis of a non-rogue revertant mutant in the rogue cv. Progreta, and the completely unusual fixation of the induced non-rogue phenotype through several generations. The reversion of the methylation status of two previously identified differentially methylated genomic sequences in the induced non-rogue mutant, confirms that the rogue paramutation is accompanied by alterations in DNA methylation. Nevertheless, unexpectedly, the induced non-rogue mutant showed to be still sensitive to paramutation.Ricardo PereiraJosé M. LeitãoMDPI AGarticlepeaPisumparamutationrogue phenotype1-ethyl-1-nitrosoureaENUGeneticsQH426-470ENGenes, Vol 12, Iss 1680, p 1680 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	pea Pisum paramutation rogue phenotype 1-ethyl-1-nitrosourea ENU Genetics QH426-470
spellingShingle	pea Pisum paramutation rogue phenotype 1-ethyl-1-nitrosourea ENU Genetics QH426-470 Ricardo Pereira José M. Leitão A Non-Rogue Mutant Line Induced by ENU Mutagenesis in Paramutated Rogue Peas (<i>Pisum sativum</i> L.) Is Still Sensitive to the Rogue Paramutation
description	The spontaneously emerging rogue phenotype in peas (<i>Pisum sativum</i> L.), characterized by narrow and pointed leaf stipula and leaflets, was the first identified case of the epigenetic phenomenon paramutation. The crosses of homozygous or heterozygous (e.g., F1) rogue plants with non-rogue (wild type) plants, produce exclusively rogue plants in the first and all subsequent generations. The fact that the wild phenotype disappears forever, is in clear contradiction with the Mendelian rules of inheritance, a situation that impedes the positional cloning of genes involved in this epigenetic phenomenon. One way of overcoming this obstacle is the identification of plant genotypes harboring naturally occurring or artificially induced neutral alleles, non-sensitive to paramutation. So far, such alleles have never been described for the pea rogue paramutation. Here, we report the induction via 1-ethyl-1-nitrosourea (ENU) mutagenesis of a non-rogue revertant mutant in the rogue cv. Progreta, and the completely unusual fixation of the induced non-rogue phenotype through several generations. The reversion of the methylation status of two previously identified differentially methylated genomic sequences in the induced non-rogue mutant, confirms that the rogue paramutation is accompanied by alterations in DNA methylation. Nevertheless, unexpectedly, the induced non-rogue mutant showed to be still sensitive to paramutation.
format	article
author	Ricardo Pereira José M. Leitão
author_facet	Ricardo Pereira José M. Leitão
author_sort	Ricardo Pereira
title	A Non-Rogue Mutant Line Induced by ENU Mutagenesis in Paramutated Rogue Peas (<i>Pisum sativum</i> L.) Is Still Sensitive to the Rogue Paramutation
title_short	A Non-Rogue Mutant Line Induced by ENU Mutagenesis in Paramutated Rogue Peas (<i>Pisum sativum</i> L.) Is Still Sensitive to the Rogue Paramutation
title_full	A Non-Rogue Mutant Line Induced by ENU Mutagenesis in Paramutated Rogue Peas (<i>Pisum sativum</i> L.) Is Still Sensitive to the Rogue Paramutation
title_fullStr	A Non-Rogue Mutant Line Induced by ENU Mutagenesis in Paramutated Rogue Peas (<i>Pisum sativum</i> L.) Is Still Sensitive to the Rogue Paramutation
title_full_unstemmed	A Non-Rogue Mutant Line Induced by ENU Mutagenesis in Paramutated Rogue Peas (<i>Pisum sativum</i> L.) Is Still Sensitive to the Rogue Paramutation
title_sort	non-rogue mutant line induced by enu mutagenesis in paramutated rogue peas (<i>pisum sativum</i> l.) is still sensitive to the rogue paramutation
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/b5509d96a1f648488178dc2c5cf01673
work_keys_str_mv	AT ricardopereira anonroguemutantlineinducedbyenumutagenesisinparamutatedroguepeasipisumsativumilisstillsensitivetotherogueparamutation AT josemleitao anonroguemutantlineinducedbyenumutagenesisinparamutatedroguepeasipisumsativumilisstillsensitivetotherogueparamutation AT ricardopereira nonroguemutantlineinducedbyenumutagenesisinparamutatedroguepeasipisumsativumilisstillsensitivetotherogueparamutation AT josemleitao nonroguemutantlineinducedbyenumutagenesisinparamutatedroguepeasipisumsativumilisstillsensitivetotherogueparamutation
_version_	1718412127195103232

A Non-Rogue Mutant Line Induced by ENU Mutagenesis in Paramutated Rogue Peas (<i>Pisum sativum</i> L.) Is Still Sensitive to the Rogue Paramutation

Ejemplares similares