Molecular characterization of sheep major histocompatibility complex (OVAR-MHC) Class II DQA1 gene in two Egyptian breeds

The major histocompatibility complex (MHC) is a large locus on vertebrate DNA that contains a set of closely related polymorphic genes coding for cell surface proteins essential for an adaptive immune system. MHC is thought to be under strong selection pressure because of its main role in infectious...

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Autores principales:	Amr M. A. Mohamed, Hemely A. Hassan, Fayza M. Aly, Alsagher O. Ali
Formato:	article
Lenguaje:	EN
Publicado:	South Valley University 2021
Materias:	dqa1 ovar-mhc ovis aries ossimi sheep saidi sheep Agriculture S Veterinary medicine SF600-1100
Acceso en línea:	https://doaj.org/article/b55aae6ab4be4e989d68b4f743b11bec
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spelling	oai:doaj.org-article:b55aae6ab4be4e989d68b4f743b11bec2021-12-02T18:53:05ZMolecular characterization of sheep major histocompatibility complex (OVAR-MHC) Class II DQA1 gene in two Egyptian breeds10.21608/SVU.2021.75495.11252535-18262535-1877https://doaj.org/article/b55aae6ab4be4e989d68b4f743b11bec2021-08-01T00:00:00Zhttps://svu.journals.ekb.eg/article_189665.htmlhttps://doaj.org/toc/2535-1826https://doaj.org/toc/2535-1877The major histocompatibility complex (MHC) is a large locus on vertebrate DNA that contains a set of closely related polymorphic genes coding for cell surface proteins essential for an adaptive immune system. MHC is thought to be under strong selection pressure because of its main role in infectious disease resistance. Thus, the pattern of different MHC genes variation should reflect this selection pressure in animal's natural populations. To test this hypothesis, we investigate the nature and frequency of polymorphism of the sheep MHC class II DQA1 gene in two different common local Egyptian sheep breeds (Ossimi and Saidi). Ovar-DQA1 gene is considered one of the most important response genes in the MHC region. In the current study, blood samples of 50 Egyptian sheep from two breeds (32 Ossimi and 18 Saidi) with different ages and sex and from seven different farms in Qena governorate were collected for DNA extraction and PCR amplification. The PCR products of 18 DQA1 alleles were examined using direct sequencing, for the detection of polymorphism in a functionally relevant domain of the DQA1 gene. Among the investigated samples the phylogenetic tree reconstruction and sequence comparison revealed different DQA1 alleles. Thirty-two sheep samples lacking DQA1, five different alleles were identified for the two breeds. The multiple sequence alignment illustrates nucleotide polymorphism and conserved regions in the identified sequences. This variation leads to a better understanding of the genetic makeup of Ovar-MHC and facilitates the selective breeding programs to determine the resistance and susceptibility to disease in sheep. Amr M. A. MohamedHemely A. HassanFayza M. Aly Alsagher O. AliSouth Valley Universityarticledqa1ovar-mhcovis ariesossimi sheepsaidi sheepAgricultureSVeterinary medicineSF600-1100ENSVU-International Journal of Veterinary Sciences, Vol 4, Iss 3, Pp 24-37 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	dqa1 ovar-mhc ovis aries ossimi sheep saidi sheep Agriculture S Veterinary medicine SF600-1100
spellingShingle	dqa1 ovar-mhc ovis aries ossimi sheep saidi sheep Agriculture S Veterinary medicine SF600-1100 Amr M. A. Mohamed Hemely A. Hassan Fayza M. Aly Alsagher O. Ali Molecular characterization of sheep major histocompatibility complex (OVAR-MHC) Class II DQA1 gene in two Egyptian breeds
description	The major histocompatibility complex (MHC) is a large locus on vertebrate DNA that contains a set of closely related polymorphic genes coding for cell surface proteins essential for an adaptive immune system. MHC is thought to be under strong selection pressure because of its main role in infectious disease resistance. Thus, the pattern of different MHC genes variation should reflect this selection pressure in animal's natural populations. To test this hypothesis, we investigate the nature and frequency of polymorphism of the sheep MHC class II DQA1 gene in two different common local Egyptian sheep breeds (Ossimi and Saidi). Ovar-DQA1 gene is considered one of the most important response genes in the MHC region. In the current study, blood samples of 50 Egyptian sheep from two breeds (32 Ossimi and 18 Saidi) with different ages and sex and from seven different farms in Qena governorate were collected for DNA extraction and PCR amplification. The PCR products of 18 DQA1 alleles were examined using direct sequencing, for the detection of polymorphism in a functionally relevant domain of the DQA1 gene. Among the investigated samples the phylogenetic tree reconstruction and sequence comparison revealed different DQA1 alleles. Thirty-two sheep samples lacking DQA1, five different alleles were identified for the two breeds. The multiple sequence alignment illustrates nucleotide polymorphism and conserved regions in the identified sequences. This variation leads to a better understanding of the genetic makeup of Ovar-MHC and facilitates the selective breeding programs to determine the resistance and susceptibility to disease in sheep.
format	article
author	Amr M. A. Mohamed Hemely A. Hassan Fayza M. Aly Alsagher O. Ali
author_facet	Amr M. A. Mohamed Hemely A. Hassan Fayza M. Aly Alsagher O. Ali
author_sort	Amr M. A. Mohamed
title	Molecular characterization of sheep major histocompatibility complex (OVAR-MHC) Class II DQA1 gene in two Egyptian breeds
title_short	Molecular characterization of sheep major histocompatibility complex (OVAR-MHC) Class II DQA1 gene in two Egyptian breeds
title_full	Molecular characterization of sheep major histocompatibility complex (OVAR-MHC) Class II DQA1 gene in two Egyptian breeds
title_fullStr	Molecular characterization of sheep major histocompatibility complex (OVAR-MHC) Class II DQA1 gene in two Egyptian breeds
title_full_unstemmed	Molecular characterization of sheep major histocompatibility complex (OVAR-MHC) Class II DQA1 gene in two Egyptian breeds
title_sort	molecular characterization of sheep major histocompatibility complex (ovar-mhc) class ii dqa1 gene in two egyptian breeds
publisher	South Valley University
publishDate	2021
url	https://doaj.org/article/b55aae6ab4be4e989d68b4f743b11bec
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Molecular characterization of sheep major histocompatibility complex (OVAR-MHC) Class II DQA1 gene in two Egyptian breeds

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