Condensation of an additive-free cell extract to mimic the conditions of live cells.

The cellular environment differs from that of reconstituted materials mainly because of the presence of highly condensed biomacromolecules. To mimic the environment and conditions in living cells, we developed a method to prepare additive-free, highly concentrated cell extracts. First, we verified t...

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Autores principales: Kei Fujiwara, Shin-ichiro M Nomura
Formato: article
Lenguaje:EN
Publicado: Public Library of Science (PLoS) 2013
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Acceso en línea:https://doaj.org/article/b562b93482b448648e8e49b9b80c6a9f
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