Generation of mutation hotspots in ageing bacterial colonies

Abstract How do ageing bacterial colonies generate adaptive mutants? Over a period of two months, we isolated on ageing colonies outgrowing mutants able to use a new carbon source, and sequenced their genomes. This allowed us to uncover exquisite details on the molecular mechanism behind their adapt...

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Autores principales:	Agnieszka Sekowska, Sofie Wendel, Emil C. Fischer, Morten H. H. Nørholm, Antoine Danchin
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2016
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/b564163529c64e73a48024b0ac5d51d4
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spelling	oai:doaj.org-article:b564163529c64e73a48024b0ac5d51d42021-12-02T15:06:06ZGeneration of mutation hotspots in ageing bacterial colonies10.1038/s41598-016-0005-42045-2322https://doaj.org/article/b564163529c64e73a48024b0ac5d51d42016-12-01T00:00:00Zhttps://doi.org/10.1038/s41598-016-0005-4https://doaj.org/toc/2045-2322Abstract How do ageing bacterial colonies generate adaptive mutants? Over a period of two months, we isolated on ageing colonies outgrowing mutants able to use a new carbon source, and sequenced their genomes. This allowed us to uncover exquisite details on the molecular mechanism behind their adaptation: most mutations were located in just a few hotspots in the genome, and over time, mutations increasingly were consistent with the involvement of 8-oxo-guanosine, formed exclusively on the transcribed strand. This work provides strong support for retromutagenesis as a general process creating adaptive mutations during ageing.Agnieszka SekowskaSofie WendelEmil C. FischerMorten H. H. NørholmAntoine DanchinNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 6, Iss 1, Pp 1-7 (2016)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Agnieszka Sekowska Sofie Wendel Emil C. Fischer Morten H. H. Nørholm Antoine Danchin Generation of mutation hotspots in ageing bacterial colonies
description	Abstract How do ageing bacterial colonies generate adaptive mutants? Over a period of two months, we isolated on ageing colonies outgrowing mutants able to use a new carbon source, and sequenced their genomes. This allowed us to uncover exquisite details on the molecular mechanism behind their adaptation: most mutations were located in just a few hotspots in the genome, and over time, mutations increasingly were consistent with the involvement of 8-oxo-guanosine, formed exclusively on the transcribed strand. This work provides strong support for retromutagenesis as a general process creating adaptive mutations during ageing.
format	article
author	Agnieszka Sekowska Sofie Wendel Emil C. Fischer Morten H. H. Nørholm Antoine Danchin
author_facet	Agnieszka Sekowska Sofie Wendel Emil C. Fischer Morten H. H. Nørholm Antoine Danchin
author_sort	Agnieszka Sekowska
title	Generation of mutation hotspots in ageing bacterial colonies
title_short	Generation of mutation hotspots in ageing bacterial colonies
title_full	Generation of mutation hotspots in ageing bacterial colonies
title_fullStr	Generation of mutation hotspots in ageing bacterial colonies
title_full_unstemmed	Generation of mutation hotspots in ageing bacterial colonies
title_sort	generation of mutation hotspots in ageing bacterial colonies
publisher	Nature Portfolio
publishDate	2016
url	https://doaj.org/article/b564163529c64e73a48024b0ac5d51d4
work_keys_str_mv	AT agnieszkasekowska generationofmutationhotspotsinageingbacterialcolonies AT sofiewendel generationofmutationhotspotsinageingbacterialcolonies AT emilcfischer generationofmutationhotspotsinageingbacterialcolonies AT mortenhhnørholm generationofmutationhotspotsinageingbacterialcolonies AT antoinedanchin generationofmutationhotspotsinageingbacterialcolonies
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Generation of mutation hotspots in ageing bacterial colonies

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