Human intrahepatic CD69 + CD8+ T cells have a tissue resident memory T cell phenotype with reduced cytolytic capacity

Abstract Tissue resident memory T cells (TRM) have been identified in various tissues, however human liver TRM to date remain unidentified. TRM can be recognized by CD69 and/or CD103 expression and may play a role in the pathology of chronic hepatitis B (CHB) and hepatitis C virus infection (CHC). L...

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Autores principales:	Femke Stelma, Annikki de Niet, Marjan J. Sinnige, Karel A. van Dort, Klaas P. J. M. van Gisbergen, Joanne Verheij, Ester M. M. van Leeuwen, Neeltje A. Kootstra, Hendrik W. Reesink
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/b56880598d574792ab00d0e87c2e8df7
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spelling	oai:doaj.org-article:b56880598d574792ab00d0e87c2e8df72021-12-02T15:06:12ZHuman intrahepatic CD69 + CD8+ T cells have a tissue resident memory T cell phenotype with reduced cytolytic capacity10.1038/s41598-017-06352-32045-2322https://doaj.org/article/b56880598d574792ab00d0e87c2e8df72017-07-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-06352-3https://doaj.org/toc/2045-2322Abstract Tissue resident memory T cells (TRM) have been identified in various tissues, however human liver TRM to date remain unidentified. TRM can be recognized by CD69 and/or CD103 expression and may play a role in the pathology of chronic hepatitis B (CHB) and hepatitis C virus infection (CHC). Liver and paired blood mononuclear cells from 17 patients (including 4 CHB and 6 CHC patients) were isolated and CD8+ T cells were comprehensively analysed by flowcytometry, immunohistochemistry and qPCR. The majority of intrahepatic CD8+ T cells expressed CD69, a marker used to identify TRM, of which a subset co-expressed CD103. CD69 + CD8+ T cells expressed low levels of S1PR1 and KLF2 and a large proportion (>90%) was CXCR6+, resembling liver TRM in mice and liver resident NK cells in human. Cytotoxic proteins were only expressed in a small fraction of liver CD69 + CD8+ T cells in patients without viral hepatitis, however, in livers from CHB patients more CD69 + CD8+ T cells were granzyme B+. In CHC patients, less intrahepatic CD69 + CD8+ T cells were Hobit+ as compared to CHB and control patients. Intrahepatic CD69 + CD8+ T cells likely TRM which have a reduced cytolytic potential. In patients with chronic viral hepatitis TRM have a distinct phenotype.Femke StelmaAnnikki de NietMarjan J. SinnigeKarel A. van DortKlaas P. J. M. van GisbergenJoanne VerheijEster M. M. van LeeuwenNeeltje A. KootstraHendrik W. ReesinkNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-10 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Femke Stelma Annikki de Niet Marjan J. Sinnige Karel A. van Dort Klaas P. J. M. van Gisbergen Joanne Verheij Ester M. M. van Leeuwen Neeltje A. Kootstra Hendrik W. Reesink Human intrahepatic CD69 + CD8+ T cells have a tissue resident memory T cell phenotype with reduced cytolytic capacity
description	Abstract Tissue resident memory T cells (TRM) have been identified in various tissues, however human liver TRM to date remain unidentified. TRM can be recognized by CD69 and/or CD103 expression and may play a role in the pathology of chronic hepatitis B (CHB) and hepatitis C virus infection (CHC). Liver and paired blood mononuclear cells from 17 patients (including 4 CHB and 6 CHC patients) were isolated and CD8+ T cells were comprehensively analysed by flowcytometry, immunohistochemistry and qPCR. The majority of intrahepatic CD8+ T cells expressed CD69, a marker used to identify TRM, of which a subset co-expressed CD103. CD69 + CD8+ T cells expressed low levels of S1PR1 and KLF2 and a large proportion (>90%) was CXCR6+, resembling liver TRM in mice and liver resident NK cells in human. Cytotoxic proteins were only expressed in a small fraction of liver CD69 + CD8+ T cells in patients without viral hepatitis, however, in livers from CHB patients more CD69 + CD8+ T cells were granzyme B+. In CHC patients, less intrahepatic CD69 + CD8+ T cells were Hobit+ as compared to CHB and control patients. Intrahepatic CD69 + CD8+ T cells likely TRM which have a reduced cytolytic potential. In patients with chronic viral hepatitis TRM have a distinct phenotype.
format	article
author	Femke Stelma Annikki de Niet Marjan J. Sinnige Karel A. van Dort Klaas P. J. M. van Gisbergen Joanne Verheij Ester M. M. van Leeuwen Neeltje A. Kootstra Hendrik W. Reesink
author_facet	Femke Stelma Annikki de Niet Marjan J. Sinnige Karel A. van Dort Klaas P. J. M. van Gisbergen Joanne Verheij Ester M. M. van Leeuwen Neeltje A. Kootstra Hendrik W. Reesink
author_sort	Femke Stelma
title	Human intrahepatic CD69 + CD8+ T cells have a tissue resident memory T cell phenotype with reduced cytolytic capacity
title_short	Human intrahepatic CD69 + CD8+ T cells have a tissue resident memory T cell phenotype with reduced cytolytic capacity
title_full	Human intrahepatic CD69 + CD8+ T cells have a tissue resident memory T cell phenotype with reduced cytolytic capacity
title_fullStr	Human intrahepatic CD69 + CD8+ T cells have a tissue resident memory T cell phenotype with reduced cytolytic capacity
title_full_unstemmed	Human intrahepatic CD69 + CD8+ T cells have a tissue resident memory T cell phenotype with reduced cytolytic capacity
title_sort	human intrahepatic cd69 + cd8+ t cells have a tissue resident memory t cell phenotype with reduced cytolytic capacity
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/b56880598d574792ab00d0e87c2e8df7
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_version_	1718388537896730624

Human intrahepatic CD69 + CD8+ T cells have a tissue resident memory T cell phenotype with reduced cytolytic capacity

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