Actively-targeted LTVSPWY peptide-modified magnetic nanoparticles for tumor imaging

Li-Yong Jie,1 Li-Li Cai,2 Le-Jian Wang,2 Xiao-Ying Ying,2 Ri-Sheng Yu,1 Min-Ming Zhang,1 Yong-Zhong Du21Department of Radiology, The Second Affiliated Hospital, Zhejiang University School of Medicine, 2College of Pharmaceutical Sciences, Zhejiang University, Hangzhou, People&#39;s Republic o...

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Autores principales:	Jie L-Y, Cai L-L, Wang L-J, Ying X-Y, Yu R-S, Zhang M-M,Du Y-Z
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2012
Materias:	Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/b57604c6666a414b87a1cbe29e4d48b0
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spelling	oai:doaj.org-article:b57604c6666a414b87a1cbe29e4d48b02021-12-02T04:23:49ZActively-targeted LTVSPWY peptide-modified magnetic nanoparticles for tumor imaging1176-91141178-2013https://doaj.org/article/b57604c6666a414b87a1cbe29e4d48b02012-07-01T00:00:00Zhttp://www.dovepress.com/actively-targeted-ltvspwy-peptide-modified-magnetic-nanoparticles-for--a10484https://doaj.org/toc/1176-9114https://doaj.org/toc/1178-2013Li-Yong Jie,1 Li-Li Cai,2 Le-Jian Wang,2 Xiao-Ying Ying,2 Ri-Sheng Yu,1 Min-Ming Zhang,1 Yong-Zhong Du21Department of Radiology, The Second Affiliated Hospital, Zhejiang University School of Medicine, 2College of Pharmaceutical Sciences, Zhejiang University, Hangzhou, People&#39;s Republic of ChinaBackground: Magnetic resonance imaging (MRI) is widely used in modern clinical medicine as a diagnostic tool, and provides noninvasive and three-dimensional visualization of biological phenomena in living organisms with high spatial and temporal resolution. Therefore, considerable attention has been paid to magnetic nanoparticles as MRI contrast agents with efficient targeting ability and cellular internalization ability, which make it possible to offer higher contrast and information-rich images for detection of disease.Methods: LTVSPWY peptide-modified PEGylated chitosan (LTVSPWY-PEG-CS) was synthesized by chemical reaction, and the chemical structure was confirmed by 1H-NMR. LTVSPWY-PEG-CS-modified magnetic nanoparticles were prepared successfully using the solvent diffusion method. Their particle size, size distribution, and zeta potential were measured by dynamic light scattering and electrophoretic mobility, and their surface morphology was investigated by transmission electron microscopy. To investigate their selective targeting ability, the cellular uptake of the LTVSPWY-PEG-CS-modified magnetic nanoparticles was observed in a cocultured system of SKOV-3 cells which overexpress HER2 and A549 cells which are HER2-negative. The in vitro cytotoxicity of these nanoparticles in SKOV-3 and A549 cells was measured using the MTT method. The SKOV-3-bearing nude mouse model was used to investigate the tumor targeting ability of the magnetic nanoparticles in vivo.Results: The average diameter and zeta potential of the LTVSPWY-PEG-CS-modified magnetic nanoparticles was 267.3 &plusmn; 23.4 nm and 30.5 &plusmn; 7.0 mV, respectively, with a narrow size distribution and spherical morphology. In vitro cytotoxicity tests demonstrated that these magnetic nanoparticles were carriers suitable for use in cancer diagnostics with low toxicity. With modification of the LTVSPWY homing peptide, magnetic nanoparticles could be selectively taken up by SKOV-3 cells overexpressing HER2 when cocultured with HER2-negative A549 cells. In vivo biodistribution results suggest that treatment with LTVSPWY-PEG-CS-modified magnetic nanoparticles/DiR enabled tumors to be identified and diagnosed more rapidly and efficiently in vivo.Conclusion: LTVSPWY-PEG-CS-modified magnetic nanoparticles are a promising contrast agent for early detection of tumors overexpressing HER2 and further diagnostic application.Keywords: LTVSPWY peptide, HER2, poly(ethylene glycol), chitosan, magnetic nanoparticles, tumor targetingJie L-YCai L-LWang L-JYing X-YYu R-SZhang M-M,Du Y-ZDove Medical PressarticleMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol 2012, Iss default, Pp 3981-3989 (2012)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine (General) R5-920
spellingShingle	Medicine (General) R5-920 Jie L-Y Cai L-L Wang L-J Ying X-Y Yu R-S Zhang M-M,Du Y-Z Actively-targeted LTVSPWY peptide-modified magnetic nanoparticles for tumor imaging
description	Li-Yong Jie,1 Li-Li Cai,2 Le-Jian Wang,2 Xiao-Ying Ying,2 Ri-Sheng Yu,1 Min-Ming Zhang,1 Yong-Zhong Du21Department of Radiology, The Second Affiliated Hospital, Zhejiang University School of Medicine, 2College of Pharmaceutical Sciences, Zhejiang University, Hangzhou, People&#39;s Republic of ChinaBackground: Magnetic resonance imaging (MRI) is widely used in modern clinical medicine as a diagnostic tool, and provides noninvasive and three-dimensional visualization of biological phenomena in living organisms with high spatial and temporal resolution. Therefore, considerable attention has been paid to magnetic nanoparticles as MRI contrast agents with efficient targeting ability and cellular internalization ability, which make it possible to offer higher contrast and information-rich images for detection of disease.Methods: LTVSPWY peptide-modified PEGylated chitosan (LTVSPWY-PEG-CS) was synthesized by chemical reaction, and the chemical structure was confirmed by 1H-NMR. LTVSPWY-PEG-CS-modified magnetic nanoparticles were prepared successfully using the solvent diffusion method. Their particle size, size distribution, and zeta potential were measured by dynamic light scattering and electrophoretic mobility, and their surface morphology was investigated by transmission electron microscopy. To investigate their selective targeting ability, the cellular uptake of the LTVSPWY-PEG-CS-modified magnetic nanoparticles was observed in a cocultured system of SKOV-3 cells which overexpress HER2 and A549 cells which are HER2-negative. The in vitro cytotoxicity of these nanoparticles in SKOV-3 and A549 cells was measured using the MTT method. The SKOV-3-bearing nude mouse model was used to investigate the tumor targeting ability of the magnetic nanoparticles in vivo.Results: The average diameter and zeta potential of the LTVSPWY-PEG-CS-modified magnetic nanoparticles was 267.3 &plusmn; 23.4 nm and 30.5 &plusmn; 7.0 mV, respectively, with a narrow size distribution and spherical morphology. In vitro cytotoxicity tests demonstrated that these magnetic nanoparticles were carriers suitable for use in cancer diagnostics with low toxicity. With modification of the LTVSPWY homing peptide, magnetic nanoparticles could be selectively taken up by SKOV-3 cells overexpressing HER2 when cocultured with HER2-negative A549 cells. In vivo biodistribution results suggest that treatment with LTVSPWY-PEG-CS-modified magnetic nanoparticles/DiR enabled tumors to be identified and diagnosed more rapidly and efficiently in vivo.Conclusion: LTVSPWY-PEG-CS-modified magnetic nanoparticles are a promising contrast agent for early detection of tumors overexpressing HER2 and further diagnostic application.Keywords: LTVSPWY peptide, HER2, poly(ethylene glycol), chitosan, magnetic nanoparticles, tumor targeting
format	article
author	Jie L-Y Cai L-L Wang L-J Ying X-Y Yu R-S Zhang M-M,Du Y-Z
author_facet	Jie L-Y Cai L-L Wang L-J Ying X-Y Yu R-S Zhang M-M,Du Y-Z
author_sort	Jie L-Y
title	Actively-targeted LTVSPWY peptide-modified magnetic nanoparticles for tumor imaging
title_short	Actively-targeted LTVSPWY peptide-modified magnetic nanoparticles for tumor imaging
title_full	Actively-targeted LTVSPWY peptide-modified magnetic nanoparticles for tumor imaging
title_fullStr	Actively-targeted LTVSPWY peptide-modified magnetic nanoparticles for tumor imaging
title_full_unstemmed	Actively-targeted LTVSPWY peptide-modified magnetic nanoparticles for tumor imaging
title_sort	actively-targeted ltvspwy peptide-modified magnetic nanoparticles for tumor imaging
publisher	Dove Medical Press
publishDate	2012
url	https://doaj.org/article/b57604c6666a414b87a1cbe29e4d48b0
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_version_	1718401268250050560

Actively-targeted LTVSPWY peptide-modified magnetic nanoparticles for tumor imaging

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