The structure of microbial community and degradation of diatoms in the deep near-bottom layer of Lake Baikal.
Insight into the role of bacteria in degradation of diatoms is important for understanding the factors and components of silica turnover in aquatic ecosystems. Using microscopic methods, it has been shown that the degree of diatom preservation and the numbers of diatom-associated bacteria in the sur...
Guardado en:
Autores principales: | , , , , , , , , , |
---|---|
Formato: | article |
Lenguaje: | EN |
Publicado: |
Public Library of Science (PLoS)
2013
|
Materias: | |
Acceso en línea: | https://doaj.org/article/b57635de8aab4659a66677495a2ef277 |
Etiquetas: |
Agregar Etiqueta
Sin Etiquetas, Sea el primero en etiquetar este registro!
|
id |
oai:doaj.org-article:b57635de8aab4659a66677495a2ef277 |
---|---|
record_format |
dspace |
spelling |
oai:doaj.org-article:b57635de8aab4659a66677495a2ef2772021-11-18T07:51:14ZThe structure of microbial community and degradation of diatoms in the deep near-bottom layer of Lake Baikal.1932-620310.1371/journal.pone.0059977https://doaj.org/article/b57635de8aab4659a66677495a2ef2772013-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/23560063/pdf/?tool=EBIhttps://doaj.org/toc/1932-6203Insight into the role of bacteria in degradation of diatoms is important for understanding the factors and components of silica turnover in aquatic ecosystems. Using microscopic methods, it has been shown that the degree of diatom preservation and the numbers of diatom-associated bacteria in the surface layer of bottom sediments decrease with depth; in the near-bottom water layer, the majority of bacteria are associated with diatom cells, being located either on the cell surface or within the cell. The structure of microbial community in the near-bottom water layer has been characterized by pyrosequencing of the 16S rRNA gene, which has revealed 149 208 unique sequences. According to the results of metagenomic analysis, the community is dominated by representatives of Proteobacteria (41.9%), Actinobacteria (16%); then follow Acidobacteria (6.9%), Cyanobacteria (5%), Bacteroidetes (4.7%), Firmicutes (2.8%), Nitrospira (1.6%), and Verrucomicrobia (1%); other phylotypes account for less than 1% each. For 18.7% of the sequences, taxonomic identification has been possible only to the Bacteria domain level. Many bacteria identified to the genus level have close relatives occurring in other aquatic ecosystems and soils. The metagenome of the bacterial community from the near-bottom water layer also contains 16S rRNA gene sequences found in previously isolated bacterial strains possessing hydrolytic enzyme activity. These data show that potential degraders of diatoms occur among the vast variety of microorganisms in the near-bottom water of Lake Baikal.Yulia R ZakharovaYuri P GalachyantsMaria I KurilkinaAlexander V LikhoshvayDarya P PetrovaSergey M ShishlyannikovNikolai V RavinAndrey V MardanovAlexey V BeletskyYelena V LikhoshwayYelena V LikhoshwayPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 8, Iss 4, p e59977 (2013) |
institution |
DOAJ |
collection |
DOAJ |
language |
EN |
topic |
Medicine R Science Q |
spellingShingle |
Medicine R Science Q Yulia R Zakharova Yuri P Galachyants Maria I Kurilkina Alexander V Likhoshvay Darya P Petrova Sergey M Shishlyannikov Nikolai V Ravin Andrey V Mardanov Alexey V Beletsky Yelena V Likhoshway Yelena V Likhoshway The structure of microbial community and degradation of diatoms in the deep near-bottom layer of Lake Baikal. |
description |
Insight into the role of bacteria in degradation of diatoms is important for understanding the factors and components of silica turnover in aquatic ecosystems. Using microscopic methods, it has been shown that the degree of diatom preservation and the numbers of diatom-associated bacteria in the surface layer of bottom sediments decrease with depth; in the near-bottom water layer, the majority of bacteria are associated with diatom cells, being located either on the cell surface or within the cell. The structure of microbial community in the near-bottom water layer has been characterized by pyrosequencing of the 16S rRNA gene, which has revealed 149 208 unique sequences. According to the results of metagenomic analysis, the community is dominated by representatives of Proteobacteria (41.9%), Actinobacteria (16%); then follow Acidobacteria (6.9%), Cyanobacteria (5%), Bacteroidetes (4.7%), Firmicutes (2.8%), Nitrospira (1.6%), and Verrucomicrobia (1%); other phylotypes account for less than 1% each. For 18.7% of the sequences, taxonomic identification has been possible only to the Bacteria domain level. Many bacteria identified to the genus level have close relatives occurring in other aquatic ecosystems and soils. The metagenome of the bacterial community from the near-bottom water layer also contains 16S rRNA gene sequences found in previously isolated bacterial strains possessing hydrolytic enzyme activity. These data show that potential degraders of diatoms occur among the vast variety of microorganisms in the near-bottom water of Lake Baikal. |
format |
article |
author |
Yulia R Zakharova Yuri P Galachyants Maria I Kurilkina Alexander V Likhoshvay Darya P Petrova Sergey M Shishlyannikov Nikolai V Ravin Andrey V Mardanov Alexey V Beletsky Yelena V Likhoshway Yelena V Likhoshway |
author_facet |
Yulia R Zakharova Yuri P Galachyants Maria I Kurilkina Alexander V Likhoshvay Darya P Petrova Sergey M Shishlyannikov Nikolai V Ravin Andrey V Mardanov Alexey V Beletsky Yelena V Likhoshway Yelena V Likhoshway |
author_sort |
Yulia R Zakharova |
title |
The structure of microbial community and degradation of diatoms in the deep near-bottom layer of Lake Baikal. |
title_short |
The structure of microbial community and degradation of diatoms in the deep near-bottom layer of Lake Baikal. |
title_full |
The structure of microbial community and degradation of diatoms in the deep near-bottom layer of Lake Baikal. |
title_fullStr |
The structure of microbial community and degradation of diatoms in the deep near-bottom layer of Lake Baikal. |
title_full_unstemmed |
The structure of microbial community and degradation of diatoms in the deep near-bottom layer of Lake Baikal. |
title_sort |
structure of microbial community and degradation of diatoms in the deep near-bottom layer of lake baikal. |
publisher |
Public Library of Science (PLoS) |
publishDate |
2013 |
url |
https://doaj.org/article/b57635de8aab4659a66677495a2ef277 |
work_keys_str_mv |
AT yuliarzakharova thestructureofmicrobialcommunityanddegradationofdiatomsinthedeepnearbottomlayeroflakebaikal AT yuripgalachyants thestructureofmicrobialcommunityanddegradationofdiatomsinthedeepnearbottomlayeroflakebaikal AT mariaikurilkina thestructureofmicrobialcommunityanddegradationofdiatomsinthedeepnearbottomlayeroflakebaikal AT alexandervlikhoshvay thestructureofmicrobialcommunityanddegradationofdiatomsinthedeepnearbottomlayeroflakebaikal AT daryappetrova thestructureofmicrobialcommunityanddegradationofdiatomsinthedeepnearbottomlayeroflakebaikal AT sergeymshishlyannikov thestructureofmicrobialcommunityanddegradationofdiatomsinthedeepnearbottomlayeroflakebaikal AT nikolaivravin thestructureofmicrobialcommunityanddegradationofdiatomsinthedeepnearbottomlayeroflakebaikal AT andreyvmardanov thestructureofmicrobialcommunityanddegradationofdiatomsinthedeepnearbottomlayeroflakebaikal AT alexeyvbeletsky thestructureofmicrobialcommunityanddegradationofdiatomsinthedeepnearbottomlayeroflakebaikal AT yelenavlikhoshway thestructureofmicrobialcommunityanddegradationofdiatomsinthedeepnearbottomlayeroflakebaikal AT yelenavlikhoshway thestructureofmicrobialcommunityanddegradationofdiatomsinthedeepnearbottomlayeroflakebaikal AT yuliarzakharova structureofmicrobialcommunityanddegradationofdiatomsinthedeepnearbottomlayeroflakebaikal AT yuripgalachyants structureofmicrobialcommunityanddegradationofdiatomsinthedeepnearbottomlayeroflakebaikal AT mariaikurilkina structureofmicrobialcommunityanddegradationofdiatomsinthedeepnearbottomlayeroflakebaikal AT alexandervlikhoshvay structureofmicrobialcommunityanddegradationofdiatomsinthedeepnearbottomlayeroflakebaikal AT daryappetrova structureofmicrobialcommunityanddegradationofdiatomsinthedeepnearbottomlayeroflakebaikal AT sergeymshishlyannikov structureofmicrobialcommunityanddegradationofdiatomsinthedeepnearbottomlayeroflakebaikal AT nikolaivravin structureofmicrobialcommunityanddegradationofdiatomsinthedeepnearbottomlayeroflakebaikal AT andreyvmardanov structureofmicrobialcommunityanddegradationofdiatomsinthedeepnearbottomlayeroflakebaikal AT alexeyvbeletsky structureofmicrobialcommunityanddegradationofdiatomsinthedeepnearbottomlayeroflakebaikal AT yelenavlikhoshway structureofmicrobialcommunityanddegradationofdiatomsinthedeepnearbottomlayeroflakebaikal AT yelenavlikhoshway structureofmicrobialcommunityanddegradationofdiatomsinthedeepnearbottomlayeroflakebaikal |
_version_ |
1718422875480784896 |