LH supplementation of ovarian stimulation protocols influences follicular fluid steroid composition contributing to the improvement of ovarian response in poor responder women

Abstract In this prospective study, we evaluated the steroid levels in 111 follicular fluids (FF) collected from 13 women stimulated with FSH monotherapy and 205 FF collected from 28 women stimulated with FSH + LH because of a previous history of hypo-responsiveness to FSH. Steroid levels were measu...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	S. Marchiani, L. Tamburrino, F. Benini, M. Pallecchi, C. Bignozzi, A. Conforti, C. Alviggi, L. Vignozzi, G. Danza, S. Pellegrini, E. Baldi
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2020
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/b57c9e2c249b4698b277d69ee7d6b324
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:b57c9e2c249b4698b277d69ee7d6b324
record_format	dspace
spelling	oai:doaj.org-article:b57c9e2c249b4698b277d69ee7d6b3242021-12-02T16:06:40ZLH supplementation of ovarian stimulation protocols influences follicular fluid steroid composition contributing to the improvement of ovarian response in poor responder women10.1038/s41598-020-69325-z2045-2322https://doaj.org/article/b57c9e2c249b4698b277d69ee7d6b3242020-07-01T00:00:00Zhttps://doi.org/10.1038/s41598-020-69325-zhttps://doaj.org/toc/2045-2322Abstract In this prospective study, we evaluated the steroid levels in 111 follicular fluids (FF) collected from 13 women stimulated with FSH monotherapy and 205 FF collected from 28 women stimulated with FSH + LH because of a previous history of hypo-responsiveness to FSH. Steroid levels were measured by HPLC/MS–MS and related to ovarian stimulation protocol, oocyte maturity, fertilization and quality of blastocysts, after individually tracking the fate of all retrieved oocytes. 17-Hydroxy-Progesterone, Androstenedione, Estradiol and Estrone were significantly higher in the FSH + LH protocol. Progesterone, 17-Hydroxy-Progesterone and Estradiol were more expressed in FF yielding a mature oocyte (p < 0.01) in the FSH + LH protocol. FF Progesterone concentration was correlated with the rate of normal fertilization in the FSH protocol. None of the FF steroids measured were associated with blastocyst quality and achievement of pregnancy. Our results indicate that LH supplementation in hypo-responsive women modifies ovarian steroid production, mimicking physiological production better and likely contributing to an improved ovarian response. Employing a correct methodological procedure to evaluate the relationship between FF steroid hormones and assisted reproduction outcomes, our study reveals that some steroids in single follicles may be helpful in predicting oocyte maturity and fertilization.S. MarchianiL. TamburrinoF. BeniniM. PallecchiC. BignozziA. ConfortiC. AlviggiL. VignozziG. DanzaS. PellegriniE. BaldiNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 10, Iss 1, Pp 1-12 (2020)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q S. Marchiani L. Tamburrino F. Benini M. Pallecchi C. Bignozzi A. Conforti C. Alviggi L. Vignozzi G. Danza S. Pellegrini E. Baldi LH supplementation of ovarian stimulation protocols influences follicular fluid steroid composition contributing to the improvement of ovarian response in poor responder women
description	Abstract In this prospective study, we evaluated the steroid levels in 111 follicular fluids (FF) collected from 13 women stimulated with FSH monotherapy and 205 FF collected from 28 women stimulated with FSH + LH because of a previous history of hypo-responsiveness to FSH. Steroid levels were measured by HPLC/MS–MS and related to ovarian stimulation protocol, oocyte maturity, fertilization and quality of blastocysts, after individually tracking the fate of all retrieved oocytes. 17-Hydroxy-Progesterone, Androstenedione, Estradiol and Estrone were significantly higher in the FSH + LH protocol. Progesterone, 17-Hydroxy-Progesterone and Estradiol were more expressed in FF yielding a mature oocyte (p < 0.01) in the FSH + LH protocol. FF Progesterone concentration was correlated with the rate of normal fertilization in the FSH protocol. None of the FF steroids measured were associated with blastocyst quality and achievement of pregnancy. Our results indicate that LH supplementation in hypo-responsive women modifies ovarian steroid production, mimicking physiological production better and likely contributing to an improved ovarian response. Employing a correct methodological procedure to evaluate the relationship between FF steroid hormones and assisted reproduction outcomes, our study reveals that some steroids in single follicles may be helpful in predicting oocyte maturity and fertilization.
format	article
author	S. Marchiani L. Tamburrino F. Benini M. Pallecchi C. Bignozzi A. Conforti C. Alviggi L. Vignozzi G. Danza S. Pellegrini E. Baldi
author_facet	S. Marchiani L. Tamburrino F. Benini M. Pallecchi C. Bignozzi A. Conforti C. Alviggi L. Vignozzi G. Danza S. Pellegrini E. Baldi
author_sort	S. Marchiani
title	LH supplementation of ovarian stimulation protocols influences follicular fluid steroid composition contributing to the improvement of ovarian response in poor responder women
title_short	LH supplementation of ovarian stimulation protocols influences follicular fluid steroid composition contributing to the improvement of ovarian response in poor responder women
title_full	LH supplementation of ovarian stimulation protocols influences follicular fluid steroid composition contributing to the improvement of ovarian response in poor responder women
title_fullStr	LH supplementation of ovarian stimulation protocols influences follicular fluid steroid composition contributing to the improvement of ovarian response in poor responder women
title_full_unstemmed	LH supplementation of ovarian stimulation protocols influences follicular fluid steroid composition contributing to the improvement of ovarian response in poor responder women
title_sort	lh supplementation of ovarian stimulation protocols influences follicular fluid steroid composition contributing to the improvement of ovarian response in poor responder women
publisher	Nature Portfolio
publishDate	2020
url	https://doaj.org/article/b57c9e2c249b4698b277d69ee7d6b324
work_keys_str_mv	AT smarchiani lhsupplementationofovarianstimulationprotocolsinfluencesfollicularfluidsteroidcompositioncontributingtotheimprovementofovarianresponseinpoorresponderwomen AT ltamburrino lhsupplementationofovarianstimulationprotocolsinfluencesfollicularfluidsteroidcompositioncontributingtotheimprovementofovarianresponseinpoorresponderwomen AT fbenini lhsupplementationofovarianstimulationprotocolsinfluencesfollicularfluidsteroidcompositioncontributingtotheimprovementofovarianresponseinpoorresponderwomen AT mpallecchi lhsupplementationofovarianstimulationprotocolsinfluencesfollicularfluidsteroidcompositioncontributingtotheimprovementofovarianresponseinpoorresponderwomen AT cbignozzi lhsupplementationofovarianstimulationprotocolsinfluencesfollicularfluidsteroidcompositioncontributingtotheimprovementofovarianresponseinpoorresponderwomen AT aconforti lhsupplementationofovarianstimulationprotocolsinfluencesfollicularfluidsteroidcompositioncontributingtotheimprovementofovarianresponseinpoorresponderwomen AT calviggi lhsupplementationofovarianstimulationprotocolsinfluencesfollicularfluidsteroidcompositioncontributingtotheimprovementofovarianresponseinpoorresponderwomen AT lvignozzi lhsupplementationofovarianstimulationprotocolsinfluencesfollicularfluidsteroidcompositioncontributingtotheimprovementofovarianresponseinpoorresponderwomen AT gdanza lhsupplementationofovarianstimulationprotocolsinfluencesfollicularfluidsteroidcompositioncontributingtotheimprovementofovarianresponseinpoorresponderwomen AT spellegrini lhsupplementationofovarianstimulationprotocolsinfluencesfollicularfluidsteroidcompositioncontributingtotheimprovementofovarianresponseinpoorresponderwomen AT ebaldi lhsupplementationofovarianstimulationprotocolsinfluencesfollicularfluidsteroidcompositioncontributingtotheimprovementofovarianresponseinpoorresponderwomen
_version_	1718384925295509504

LH supplementation of ovarian stimulation protocols influences follicular fluid steroid composition contributing to the improvement of ovarian response in poor responder women

Ejemplares similares