Isolation and Identification of a Rumen <i>Lactobacillus</i> Bacteria and Its Degradation Potential of Gossypol in Cottonseed Meal during Solid-State Fermentation

Cottonseed meal (CSM) is an important protein feed source for dairy cows. Its inclusion in ruminant diets is limited due to the presence of the highly toxic gossypol though rumen microorganisms are believed to be capable of gossypol degrading and transforming. The objective of the present study was...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Wei-Kang Wang, Wen-Juan Li, Qi-Chao Wu, Yan-Lu Wang, Sheng-Li Li, Hong-Jian Yang
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	gossypol rumen solid-state fermentation cottonseed meal Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/b581383d654045ff9c489c0e05bcd76c
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:b581383d654045ff9c489c0e05bcd76c
record_format	dspace
spelling	oai:doaj.org-article:b581383d654045ff9c489c0e05bcd76c2021-11-25T18:24:11ZIsolation and Identification of a Rumen <i>Lactobacillus</i> Bacteria and Its Degradation Potential of Gossypol in Cottonseed Meal during Solid-State Fermentation10.3390/microorganisms91122002076-2607https://doaj.org/article/b581383d654045ff9c489c0e05bcd76c2021-10-01T00:00:00Zhttps://www.mdpi.com/2076-2607/9/11/2200https://doaj.org/toc/2076-2607Cottonseed meal (CSM) is an important protein feed source for dairy cows. Its inclusion in ruminant diets is limited due to the presence of the highly toxic gossypol though rumen microorganisms are believed to be capable of gossypol degrading and transforming. The objective of the present study was to isolate the gossypol-degrading bacteria from the rumen contents and to assess its potential for gossypol degradation in vitro. A strain named <i>Lactobacillus agilis</i> WWK129 was anaerobically isolated from dairy cows after mixed rumen microorganisms were grown on a substrate with gossypol as the sole carbon source. Furthermore, the strain was applied at 5% inoculum concentration in vitro to continuously ferment CSM at 39 °C for five days, and it presented gossypol degradability as high as 83%. Meanwhile, the CSM contents of crude protein, essential amino acids increased significantly along with the increase of lactic acid yield (<i>p</i> < 0.01). Compared with the original CSM, the fermented CSM contents of neutral detergent fiber and acid detergent fiber was remarkably decreased after the anaerobic fermentation (<i>p</i> < 0.01). In brief, the <i>Lactobacillus</i> strain isolated from the rumen is not only of great importance for gossypol biodegradation of CSM, but it could also be used to further explore the role of rumen microorganisms in gossypol degradation by the ruminants.Wei-Kang WangWen-Juan LiQi-Chao WuYan-Lu WangSheng-Li LiHong-Jian YangMDPI AGarticlegossypolrumensolid-state fermentationcottonseed mealBiology (General)QH301-705.5ENMicroorganisms, Vol 9, Iss 2200, p 2200 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	gossypol rumen solid-state fermentation cottonseed meal Biology (General) QH301-705.5
spellingShingle	gossypol rumen solid-state fermentation cottonseed meal Biology (General) QH301-705.5 Wei-Kang Wang Wen-Juan Li Qi-Chao Wu Yan-Lu Wang Sheng-Li Li Hong-Jian Yang Isolation and Identification of a Rumen <i>Lactobacillus</i> Bacteria and Its Degradation Potential of Gossypol in Cottonseed Meal during Solid-State Fermentation
description	Cottonseed meal (CSM) is an important protein feed source for dairy cows. Its inclusion in ruminant diets is limited due to the presence of the highly toxic gossypol though rumen microorganisms are believed to be capable of gossypol degrading and transforming. The objective of the present study was to isolate the gossypol-degrading bacteria from the rumen contents and to assess its potential for gossypol degradation in vitro. A strain named <i>Lactobacillus agilis</i> WWK129 was anaerobically isolated from dairy cows after mixed rumen microorganisms were grown on a substrate with gossypol as the sole carbon source. Furthermore, the strain was applied at 5% inoculum concentration in vitro to continuously ferment CSM at 39 °C for five days, and it presented gossypol degradability as high as 83%. Meanwhile, the CSM contents of crude protein, essential amino acids increased significantly along with the increase of lactic acid yield (<i>p</i> < 0.01). Compared with the original CSM, the fermented CSM contents of neutral detergent fiber and acid detergent fiber was remarkably decreased after the anaerobic fermentation (<i>p</i> < 0.01). In brief, the <i>Lactobacillus</i> strain isolated from the rumen is not only of great importance for gossypol biodegradation of CSM, but it could also be used to further explore the role of rumen microorganisms in gossypol degradation by the ruminants.
format	article
author	Wei-Kang Wang Wen-Juan Li Qi-Chao Wu Yan-Lu Wang Sheng-Li Li Hong-Jian Yang
author_facet	Wei-Kang Wang Wen-Juan Li Qi-Chao Wu Yan-Lu Wang Sheng-Li Li Hong-Jian Yang
author_sort	Wei-Kang Wang
title	Isolation and Identification of a Rumen <i>Lactobacillus</i> Bacteria and Its Degradation Potential of Gossypol in Cottonseed Meal during Solid-State Fermentation
title_short	Isolation and Identification of a Rumen <i>Lactobacillus</i> Bacteria and Its Degradation Potential of Gossypol in Cottonseed Meal during Solid-State Fermentation
title_full	Isolation and Identification of a Rumen <i>Lactobacillus</i> Bacteria and Its Degradation Potential of Gossypol in Cottonseed Meal during Solid-State Fermentation
title_fullStr	Isolation and Identification of a Rumen <i>Lactobacillus</i> Bacteria and Its Degradation Potential of Gossypol in Cottonseed Meal during Solid-State Fermentation
title_full_unstemmed	Isolation and Identification of a Rumen <i>Lactobacillus</i> Bacteria and Its Degradation Potential of Gossypol in Cottonseed Meal during Solid-State Fermentation
title_sort	isolation and identification of a rumen <i>lactobacillus</i> bacteria and its degradation potential of gossypol in cottonseed meal during solid-state fermentation
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/b581383d654045ff9c489c0e05bcd76c
work_keys_str_mv	AT weikangwang isolationandidentificationofarumenilactobacillusibacteriaanditsdegradationpotentialofgossypolincottonseedmealduringsolidstatefermentation AT wenjuanli isolationandidentificationofarumenilactobacillusibacteriaanditsdegradationpotentialofgossypolincottonseedmealduringsolidstatefermentation AT qichaowu isolationandidentificationofarumenilactobacillusibacteriaanditsdegradationpotentialofgossypolincottonseedmealduringsolidstatefermentation AT yanluwang isolationandidentificationofarumenilactobacillusibacteriaanditsdegradationpotentialofgossypolincottonseedmealduringsolidstatefermentation AT shenglili isolationandidentificationofarumenilactobacillusibacteriaanditsdegradationpotentialofgossypolincottonseedmealduringsolidstatefermentation AT hongjianyang isolationandidentificationofarumenilactobacillusibacteriaanditsdegradationpotentialofgossypolincottonseedmealduringsolidstatefermentation
_version_	1718411219879067648

Isolation and Identification of a Rumen <i>Lactobacillus</i> Bacteria and Its Degradation Potential of Gossypol in Cottonseed Meal during Solid-State Fermentation

Ejemplares similares