Role of structure and glycosylation of adsorbed protein films in biolubrication.

Water forms the basis of lubrication in the human body, but is unable to provide sufficient lubrication without additives. The importance of biolubrication becomes evident upon aging and disease, particularly under conditions that affect secretion or composition of body fluids. Insufficient biolubri...

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Autores principales:	Deepak H Veeregowda, Henk J Busscher, Arjan Vissink, Derk-Jan Jager, Prashant K Sharma, Henny C van der Mei
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2012
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/b587fa63a1c14a9192ac4f31f937a425
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spelling	oai:doaj.org-article:b587fa63a1c14a9192ac4f31f937a4252021-11-18T07:08:39ZRole of structure and glycosylation of adsorbed protein films in biolubrication.1932-620310.1371/journal.pone.0042600https://doaj.org/article/b587fa63a1c14a9192ac4f31f937a4252012-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/22916138/pdf/?tool=EBIhttps://doaj.org/toc/1932-6203Water forms the basis of lubrication in the human body, but is unable to provide sufficient lubrication without additives. The importance of biolubrication becomes evident upon aging and disease, particularly under conditions that affect secretion or composition of body fluids. Insufficient biolubrication, may impede proper speech, mastication and swallowing, underlie excessive friction and wear of articulating cartilage surfaces in hips and knees, cause vaginal dryness, and result in dry, irritated eyes. Currently, our understanding of biolubrication is insufficient to design effective therapeutics to restore biolubrication. Aim of this study was to establish the role of structure and glycosylation of adsorbed protein films in biolubrication, taking the oral cavity as a model and making use of its dynamics with daily perturbations due to different glandular secretions, speech, drinking and eating, and tooth brushing. Using different surface analytical techniques (a quartz crystal microbalance with dissipation monitoring, colloidal probe atomic force microscopy, contact angle measurements and X-ray photo-electron spectroscopy), we demonstrated that adsorbed salivary conditioning films in vitro are more lubricious when their hydrophilicity and degree of glycosylation increase, meanwhile decreasing their structural softness. High-molecular-weight, glycosylated proteins adsorbing in loops and trains, are described as necessary scaffolds impeding removal of water during loading of articulating surfaces. Comparing in vitro and in vivo water contact angles measured intra-orally, these findings were extrapolated to the in vivo situation. Accordingly, lubricating properties of teeth, as perceived in 20 volunteers comprising of equal numbers of male and female subjects, could be related with structural softness and glycosylation of adsorbed protein films on tooth surfaces. Summarizing, biolubrication is due to a combination of structure and glycosylation of adsorbed protein films, providing an important clue to design effective therapeutics to restore biolubrication in patients with insufficient biolubrication.Deepak H VeeregowdaHenk J BusscherArjan VissinkDerk-Jan JagerPrashant K SharmaHenny C van der MeiPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 7, Iss 8, p e42600 (2012)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Deepak H Veeregowda Henk J Busscher Arjan Vissink Derk-Jan Jager Prashant K Sharma Henny C van der Mei Role of structure and glycosylation of adsorbed protein films in biolubrication.
description	Water forms the basis of lubrication in the human body, but is unable to provide sufficient lubrication without additives. The importance of biolubrication becomes evident upon aging and disease, particularly under conditions that affect secretion or composition of body fluids. Insufficient biolubrication, may impede proper speech, mastication and swallowing, underlie excessive friction and wear of articulating cartilage surfaces in hips and knees, cause vaginal dryness, and result in dry, irritated eyes. Currently, our understanding of biolubrication is insufficient to design effective therapeutics to restore biolubrication. Aim of this study was to establish the role of structure and glycosylation of adsorbed protein films in biolubrication, taking the oral cavity as a model and making use of its dynamics with daily perturbations due to different glandular secretions, speech, drinking and eating, and tooth brushing. Using different surface analytical techniques (a quartz crystal microbalance with dissipation monitoring, colloidal probe atomic force microscopy, contact angle measurements and X-ray photo-electron spectroscopy), we demonstrated that adsorbed salivary conditioning films in vitro are more lubricious when their hydrophilicity and degree of glycosylation increase, meanwhile decreasing their structural softness. High-molecular-weight, glycosylated proteins adsorbing in loops and trains, are described as necessary scaffolds impeding removal of water during loading of articulating surfaces. Comparing in vitro and in vivo water contact angles measured intra-orally, these findings were extrapolated to the in vivo situation. Accordingly, lubricating properties of teeth, as perceived in 20 volunteers comprising of equal numbers of male and female subjects, could be related with structural softness and glycosylation of adsorbed protein films on tooth surfaces. Summarizing, biolubrication is due to a combination of structure and glycosylation of adsorbed protein films, providing an important clue to design effective therapeutics to restore biolubrication in patients with insufficient biolubrication.
format	article
author	Deepak H Veeregowda Henk J Busscher Arjan Vissink Derk-Jan Jager Prashant K Sharma Henny C van der Mei
author_facet	Deepak H Veeregowda Henk J Busscher Arjan Vissink Derk-Jan Jager Prashant K Sharma Henny C van der Mei
author_sort	Deepak H Veeregowda
title	Role of structure and glycosylation of adsorbed protein films in biolubrication.
title_short	Role of structure and glycosylation of adsorbed protein films in biolubrication.
title_full	Role of structure and glycosylation of adsorbed protein films in biolubrication.
title_fullStr	Role of structure and glycosylation of adsorbed protein films in biolubrication.
title_full_unstemmed	Role of structure and glycosylation of adsorbed protein films in biolubrication.
title_sort	role of structure and glycosylation of adsorbed protein films in biolubrication.
publisher	Public Library of Science (PLoS)
publishDate	2012
url	https://doaj.org/article/b587fa63a1c14a9192ac4f31f937a425
work_keys_str_mv	AT deepakhveeregowda roleofstructureandglycosylationofadsorbedproteinfilmsinbiolubrication AT henkjbusscher roleofstructureandglycosylationofadsorbedproteinfilmsinbiolubrication AT arjanvissink roleofstructureandglycosylationofadsorbedproteinfilmsinbiolubrication AT derkjanjager roleofstructureandglycosylationofadsorbedproteinfilmsinbiolubrication AT prashantksharma roleofstructureandglycosylationofadsorbedproteinfilmsinbiolubrication AT hennycvandermei roleofstructureandglycosylationofadsorbedproteinfilmsinbiolubrication
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Role of structure and glycosylation of adsorbed protein films in biolubrication.

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