Plasma metabolomics profiling of maintenance hemodialysis based on capillary electrophoresis - time of flight mass spectrometry

Abstract Uremia has been a rapidly increasing health problem in China. Hemodialysis (HD) is the main renal replacement therapy for uremia. The results of large-scale clinical trials have shown that the HD pattern is crucial for long-term prognosis of maintenance hemodialysis (MHD) in uremic patients...

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Autores principales:	Shuxin Liu, Lichao Wang, Chunxiu Hu, Xin Huang, Hong Liu, Qiuhui Xuan, Xiaohui Lin, Xiaojun Peng, Xin Lu, Ming Chang, Guowang Xu
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/b593f27e563346f29af17d82155eaf5e
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spelling	oai:doaj.org-article:b593f27e563346f29af17d82155eaf5e2021-12-02T11:40:13ZPlasma metabolomics profiling of maintenance hemodialysis based on capillary electrophoresis - time of flight mass spectrometry10.1038/s41598-017-08327-w2045-2322https://doaj.org/article/b593f27e563346f29af17d82155eaf5e2017-08-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-08327-whttps://doaj.org/toc/2045-2322Abstract Uremia has been a rapidly increasing health problem in China. Hemodialysis (HD) is the main renal replacement therapy for uremia. The results of large-scale clinical trials have shown that the HD pattern is crucial for long-term prognosis of maintenance hemodialysis (MHD) in uremic patients. Plasma metabolism is very important for revealing the biological insights linked to the therapeutic effects of the HD pattern on uremia. Alteration of plasma metabolites in uremic patients in response to HD therapy has been reported. However, HD-pattern-dependent changes in plasma metabolites remain poorly understood. To this end, a capillary electrophoresis-time of flight mass spectrometry (CE-TOF/MS)-based metabolomics method was performed to systemically study the differences between HD and high flux hemodialysis (HFD) on plasma metabolite changes in patients. Three hundred and one plasma samples from three independent human cohorts (i.e., healthy controls, patients with pre-HD/post-HD, and patients with pre-HFD/post-HFD) were used in this study. Metabolites significantly changed (p < 0.05) after a single HD or HFD process. However, 11 uremic retention solutes could be more efficiently removed by HFD. Our findings indicate that a CE-TOF/MS-based metabolomics approach is promising for providing novel insights into understanding the effects of different dialysis methods on metabolite alterations of uremia.Shuxin LiuLichao WangChunxiu HuXin HuangHong LiuQiuhui XuanXiaohui LinXiaojun PengXin LuMing ChangGuowang XuNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-11 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Shuxin Liu Lichao Wang Chunxiu Hu Xin Huang Hong Liu Qiuhui Xuan Xiaohui Lin Xiaojun Peng Xin Lu Ming Chang Guowang Xu Plasma metabolomics profiling of maintenance hemodialysis based on capillary electrophoresis - time of flight mass spectrometry
description	Abstract Uremia has been a rapidly increasing health problem in China. Hemodialysis (HD) is the main renal replacement therapy for uremia. The results of large-scale clinical trials have shown that the HD pattern is crucial for long-term prognosis of maintenance hemodialysis (MHD) in uremic patients. Plasma metabolism is very important for revealing the biological insights linked to the therapeutic effects of the HD pattern on uremia. Alteration of plasma metabolites in uremic patients in response to HD therapy has been reported. However, HD-pattern-dependent changes in plasma metabolites remain poorly understood. To this end, a capillary electrophoresis-time of flight mass spectrometry (CE-TOF/MS)-based metabolomics method was performed to systemically study the differences between HD and high flux hemodialysis (HFD) on plasma metabolite changes in patients. Three hundred and one plasma samples from three independent human cohorts (i.e., healthy controls, patients with pre-HD/post-HD, and patients with pre-HFD/post-HFD) were used in this study. Metabolites significantly changed (p < 0.05) after a single HD or HFD process. However, 11 uremic retention solutes could be more efficiently removed by HFD. Our findings indicate that a CE-TOF/MS-based metabolomics approach is promising for providing novel insights into understanding the effects of different dialysis methods on metabolite alterations of uremia.
format	article
author	Shuxin Liu Lichao Wang Chunxiu Hu Xin Huang Hong Liu Qiuhui Xuan Xiaohui Lin Xiaojun Peng Xin Lu Ming Chang Guowang Xu
author_facet	Shuxin Liu Lichao Wang Chunxiu Hu Xin Huang Hong Liu Qiuhui Xuan Xiaohui Lin Xiaojun Peng Xin Lu Ming Chang Guowang Xu
author_sort	Shuxin Liu
title	Plasma metabolomics profiling of maintenance hemodialysis based on capillary electrophoresis - time of flight mass spectrometry
title_short	Plasma metabolomics profiling of maintenance hemodialysis based on capillary electrophoresis - time of flight mass spectrometry
title_full	Plasma metabolomics profiling of maintenance hemodialysis based on capillary electrophoresis - time of flight mass spectrometry
title_fullStr	Plasma metabolomics profiling of maintenance hemodialysis based on capillary electrophoresis - time of flight mass spectrometry
title_full_unstemmed	Plasma metabolomics profiling of maintenance hemodialysis based on capillary electrophoresis - time of flight mass spectrometry
title_sort	plasma metabolomics profiling of maintenance hemodialysis based on capillary electrophoresis - time of flight mass spectrometry
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/b593f27e563346f29af17d82155eaf5e
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Plasma metabolomics profiling of maintenance hemodialysis based on capillary electrophoresis - time of flight mass spectrometry

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