Survey on Methods for Investigating Protein Functionality and Related Molecular Characteristics

Proteins from various sources are widely used in the food industry due to their unique functional performances in food products. The functional properties of proteins are somehow dictated by their molecular characteristics, but the exact relationship is not fully understood. This review gives a tang...

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Autores principales:	Yuqi Zhang, Siddharth Sharan, Åsmund Rinnan, Vibeke Orlien
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	proteins functional properties molecular characterization protein structure spectroscopy toolbox Chemical technology TP1-1185
Acceso en línea:	https://doaj.org/article/b5976b304c274b6198549e4cd4918380
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spelling	oai:doaj.org-article:b5976b304c274b6198549e4cd49183802021-11-25T17:36:23ZSurvey on Methods for Investigating Protein Functionality and Related Molecular Characteristics10.3390/foods101128482304-8158https://doaj.org/article/b5976b304c274b6198549e4cd49183802021-11-01T00:00:00Zhttps://www.mdpi.com/2304-8158/10/11/2848https://doaj.org/toc/2304-8158Proteins from various sources are widely used in the food industry due to their unique functional performances in food products. The functional properties of proteins are somehow dictated by their molecular characteristics, but the exact relationship is not fully understood. This review gives a tangible overview of the methods currently available for determining protein functionality and related molecular characteristics in order to support further research on protein ingredients. The measurements of protein functionality include solubility, water holding capacity, oil holding capacity, emulsion property, foam property, and gelation. This review also provides a description of different methods of molecular characteristics including electrophoresis, surface hydrophobicity and charge, molecular interaction, and thermal property measurement. Additionally, we have put significant emphasis on spectroscopic methods (ultraviolet-visible, Fourier transform infrared, Raman, circular dichroism, fluorescence and nuclear magnetic resonance). In conclusion, first and foremost, there is a need to agree on a standardization of the analytical methods for assessing functional properties. Moreover, it is mandatory to couple different analyses of molecular characteristics to measure and monitor the structural changes obtained by different processing methods in order to gain knowledge about the relationship with functionality. Ideally, a toolbox of protein analytical methods to measure molecular characteristics and functionality should be established to be used in a strategic design of protein ingredients.Yuqi ZhangSiddharth SharanÅsmund RinnanVibeke OrlienMDPI AGarticleproteinsfunctional propertiesmolecular characterizationprotein structurespectroscopytoolboxChemical technologyTP1-1185ENFoods, Vol 10, Iss 2848, p 2848 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	proteins functional properties molecular characterization protein structure spectroscopy toolbox Chemical technology TP1-1185
spellingShingle	proteins functional properties molecular characterization protein structure spectroscopy toolbox Chemical technology TP1-1185 Yuqi Zhang Siddharth Sharan Åsmund Rinnan Vibeke Orlien Survey on Methods for Investigating Protein Functionality and Related Molecular Characteristics
description	Proteins from various sources are widely used in the food industry due to their unique functional performances in food products. The functional properties of proteins are somehow dictated by their molecular characteristics, but the exact relationship is not fully understood. This review gives a tangible overview of the methods currently available for determining protein functionality and related molecular characteristics in order to support further research on protein ingredients. The measurements of protein functionality include solubility, water holding capacity, oil holding capacity, emulsion property, foam property, and gelation. This review also provides a description of different methods of molecular characteristics including electrophoresis, surface hydrophobicity and charge, molecular interaction, and thermal property measurement. Additionally, we have put significant emphasis on spectroscopic methods (ultraviolet-visible, Fourier transform infrared, Raman, circular dichroism, fluorescence and nuclear magnetic resonance). In conclusion, first and foremost, there is a need to agree on a standardization of the analytical methods for assessing functional properties. Moreover, it is mandatory to couple different analyses of molecular characteristics to measure and monitor the structural changes obtained by different processing methods in order to gain knowledge about the relationship with functionality. Ideally, a toolbox of protein analytical methods to measure molecular characteristics and functionality should be established to be used in a strategic design of protein ingredients.
format	article
author	Yuqi Zhang Siddharth Sharan Åsmund Rinnan Vibeke Orlien
author_facet	Yuqi Zhang Siddharth Sharan Åsmund Rinnan Vibeke Orlien
author_sort	Yuqi Zhang
title	Survey on Methods for Investigating Protein Functionality and Related Molecular Characteristics
title_short	Survey on Methods for Investigating Protein Functionality and Related Molecular Characteristics
title_full	Survey on Methods for Investigating Protein Functionality and Related Molecular Characteristics
title_fullStr	Survey on Methods for Investigating Protein Functionality and Related Molecular Characteristics
title_full_unstemmed	Survey on Methods for Investigating Protein Functionality and Related Molecular Characteristics
title_sort	survey on methods for investigating protein functionality and related molecular characteristics
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/b5976b304c274b6198549e4cd4918380
work_keys_str_mv	AT yuqizhang surveyonmethodsforinvestigatingproteinfunctionalityandrelatedmolecularcharacteristics AT siddharthsharan surveyonmethodsforinvestigatingproteinfunctionalityandrelatedmolecularcharacteristics AT asmundrinnan surveyonmethodsforinvestigatingproteinfunctionalityandrelatedmolecularcharacteristics AT vibekeorlien surveyonmethodsforinvestigatingproteinfunctionalityandrelatedmolecularcharacteristics
_version_	1718412187926528000

Survey on Methods for Investigating Protein Functionality and Related Molecular Characteristics

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