Investigation of endocytosis and cytotoxicity of poly-d, l-lactide-poly(ethylene glycol) micro/nano-particles in osteoblast cells

Weijia Wang, Shaobing Zhou, Laiyang Guo, Wei Zhi, Xiaohong Li, Jie WengSchool of Materials Science and Engineering, Key Laboratory of Advanced Technologies of Material, Ministry of Education, Southwest Jiaotong University, Chengdou, Sichuan, People&rsquo;s Republic of ChinaAbstract: Biodegra...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Weijia Wang, Shaobing Zhou, Laiyang Guo, et al
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2010
Materias:	Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/b599d6354bc2474da56f2ff17e519217
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:b599d6354bc2474da56f2ff17e519217
record_format	dspace
spelling	oai:doaj.org-article:b599d6354bc2474da56f2ff17e5192172021-12-02T05:02:08ZInvestigation of endocytosis and cytotoxicity of poly-d, l-lactide-poly(ethylene glycol) micro/nano-particles in osteoblast cells1176-91141178-2013https://doaj.org/article/b599d6354bc2474da56f2ff17e5192172010-07-01T00:00:00Zhttp://www.dovepress.com/investigation-of-endocytosis-and-cytotoxicity-of-poly-d-l-lactide-poly-a4947https://doaj.org/toc/1176-9114https://doaj.org/toc/1178-2013Weijia Wang, Shaobing Zhou, Laiyang Guo, Wei Zhi, Xiaohong Li, Jie WengSchool of Materials Science and Engineering, Key Laboratory of Advanced Technologies of Material, Ministry of Education, Southwest Jiaotong University, Chengdou, Sichuan, People&rsquo;s Republic of ChinaAbstract: Biodegradable polymer particles present a promising approach for intracellular delivery of drugs, proteins, and nucleic acids. Poly-d,l-lactide-poly(ethylene glycol) (PELA) copolymers with different weight ratios of polyethylene glycol (PEG) were used as drug carriers in the present study. PELA particles entrapped with fluorescein isothiocyanate (FITC) as a fluorescent marker were formulated using a double emulsion-solvent evaporation technique. The size and morphology of the particles were observed with scanning electron microscope (SEM), atomic force microscope (AFM), and laser diffraction particle size analyzer (LDPSA). The purpose in the present work was to investigate the cytotoxicity and the process of endocytosis of PELA particles with different contents of PEG and variable particle size using rat osteoblasts (OBs). The cytotoxicity of the particles was investigated using 5-dimethylthiazol-2-yl-2,5-diphenyltetrazolium bromide (MTT) assay and flow cytometry. Results indicate that as the content of PEG in the polymer increased, so did cell survival. Endocytosis was observed through a light microscope and a fluorescence microscope; intracellular uptake and retention were determined quantitatively using fluorescence spectrophotometer (FSP). The results showed that as PEG content in PELA copolymer increased, there was a reduction in endocytosis of nanoparticles in osteoblasts.Keywords: nanoparticles, endocytosis, cytotoxicity, intracellular traf?cking, drug delivery Weijia WangShaobing ZhouLaiyang Guoet alDove Medical PressarticleMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol 2010, Iss default, Pp 557-566 (2010)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine (General) R5-920
spellingShingle	Medicine (General) R5-920 Weijia Wang Shaobing Zhou Laiyang Guo et al Investigation of endocytosis and cytotoxicity of poly-d, l-lactide-poly(ethylene glycol) micro/nano-particles in osteoblast cells
description	Weijia Wang, Shaobing Zhou, Laiyang Guo, Wei Zhi, Xiaohong Li, Jie WengSchool of Materials Science and Engineering, Key Laboratory of Advanced Technologies of Material, Ministry of Education, Southwest Jiaotong University, Chengdou, Sichuan, People&rsquo;s Republic of ChinaAbstract: Biodegradable polymer particles present a promising approach for intracellular delivery of drugs, proteins, and nucleic acids. Poly-d,l-lactide-poly(ethylene glycol) (PELA) copolymers with different weight ratios of polyethylene glycol (PEG) were used as drug carriers in the present study. PELA particles entrapped with fluorescein isothiocyanate (FITC) as a fluorescent marker were formulated using a double emulsion-solvent evaporation technique. The size and morphology of the particles were observed with scanning electron microscope (SEM), atomic force microscope (AFM), and laser diffraction particle size analyzer (LDPSA). The purpose in the present work was to investigate the cytotoxicity and the process of endocytosis of PELA particles with different contents of PEG and variable particle size using rat osteoblasts (OBs). The cytotoxicity of the particles was investigated using 5-dimethylthiazol-2-yl-2,5-diphenyltetrazolium bromide (MTT) assay and flow cytometry. Results indicate that as the content of PEG in the polymer increased, so did cell survival. Endocytosis was observed through a light microscope and a fluorescence microscope; intracellular uptake and retention were determined quantitatively using fluorescence spectrophotometer (FSP). The results showed that as PEG content in PELA copolymer increased, there was a reduction in endocytosis of nanoparticles in osteoblasts.Keywords: nanoparticles, endocytosis, cytotoxicity, intracellular traf?cking, drug delivery
format	article
author	Weijia Wang Shaobing Zhou Laiyang Guo et al
author_facet	Weijia Wang Shaobing Zhou Laiyang Guo et al
author_sort	Weijia Wang
title	Investigation of endocytosis and cytotoxicity of poly-d, l-lactide-poly(ethylene glycol) micro/nano-particles in osteoblast cells
title_short	Investigation of endocytosis and cytotoxicity of poly-d, l-lactide-poly(ethylene glycol) micro/nano-particles in osteoblast cells
title_full	Investigation of endocytosis and cytotoxicity of poly-d, l-lactide-poly(ethylene glycol) micro/nano-particles in osteoblast cells
title_fullStr	Investigation of endocytosis and cytotoxicity of poly-d, l-lactide-poly(ethylene glycol) micro/nano-particles in osteoblast cells
title_full_unstemmed	Investigation of endocytosis and cytotoxicity of poly-d, l-lactide-poly(ethylene glycol) micro/nano-particles in osteoblast cells
title_sort	investigation of endocytosis and cytotoxicity of poly-d, l-lactide-poly(ethylene glycol) micro/nano-particles in osteoblast cells
publisher	Dove Medical Press
publishDate	2010
url	https://doaj.org/article/b599d6354bc2474da56f2ff17e519217
work_keys_str_mv	AT weijiawang investigationofendocytosisandcytotoxicityofpolydllactidepolyethyleneglycolmicronanoparticlesinosteoblastcells AT shaobingzhou investigationofendocytosisandcytotoxicityofpolydllactidepolyethyleneglycolmicronanoparticlesinosteoblastcells AT laiyangguo investigationofendocytosisandcytotoxicityofpolydllactidepolyethyleneglycolmicronanoparticlesinosteoblastcells AT etal investigationofendocytosisandcytotoxicityofpolydllactidepolyethyleneglycolmicronanoparticlesinosteoblastcells
_version_	1718400789289893888

Investigation of endocytosis and cytotoxicity of poly-d, l-lactide-poly(ethylene glycol) micro/nano-particles in osteoblast cells

Ejemplares similares