Age-related changes in the retinal pigment epithelium (RPE).

<h4>Background</h4>Age-related changes in the retina are often accompanied by visual impairment but their mechanistic details remain poorly understood.<h4>Methodology</h4>Proteomic studies were pursued toward a better molecular understanding of retinal pigment epithelium (RPE...

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Autores principales:	Xiaorong Gu, Nikolas J Neric, John S Crabb, John W Crabb, Sanjoy K Bhattacharya, Mary E Rayborn, Joe G Hollyfield, Vera L Bonilha
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2012
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/b599f0f67e2a4faf83d2afc1cab678f1
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spelling	oai:doaj.org-article:b599f0f67e2a4faf83d2afc1cab678f12021-11-18T07:15:50ZAge-related changes in the retinal pigment epithelium (RPE).1932-620310.1371/journal.pone.0038673https://doaj.org/article/b599f0f67e2a4faf83d2afc1cab678f12012-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/22701690/?tool=EBIhttps://doaj.org/toc/1932-6203<h4>Background</h4>Age-related changes in the retina are often accompanied by visual impairment but their mechanistic details remain poorly understood.<h4>Methodology</h4>Proteomic studies were pursued toward a better molecular understanding of retinal pigment epithelium (RPE) aging mechanisms. RPE cells were isolated from young adults (3-4 month-old) and old (24-25 month-old) F344BN rats, and separated into subcellular fractions containing apical microvilli (MV) and RPE cell bodies (CB) lacking their apical microvilli. Proteins were extracted in detergent, separated by SDS-PAGE, digested in situ with trypsin and analyzed by LC MS/MS. Select proteins detected in young and old rat RPE were further studied using immunofluorescence and Western blot analysis.<h4>Principal findings</h4>A total of 356 proteins were identified in RPE MV from young and 378 in RPE MV from old rats, 48% of which were common to each age group. A total of 897 proteins were identified in RPE CB from young rats and 675 in old CB, 56% of which were common to each age group. Several of the identified proteins, including proteins involved in response to oxidative stress, displayed both quantitative and qualitative changes in overall abundance during RPE aging. Numerous proteins were identified for the first time in the RPE. One such protein, collectrin, was localized to the apical membrane of apical brush border of proximal tubules where it likely regulates several amino acid transporters. Elsewhere, collectrin is involved in pancreatic β cell proliferation and insulin secretion. In the RPE, collectrin expression was significantly modulated during RPE aging. Another age-regulated, newly described protein was DJ-1, a protein extensively studied in brain where oxidative stress-related functions have been described.<h4>Conclusions/significance</h4>The data presented here reveals specific changes in the RPE during aging, providing the first protein database of RPE aging, which will facilitate future studies of age-related retinal diseases.Xiaorong GuNikolas J NericJohn S CrabbJohn W CrabbSanjoy K BhattacharyaMary E RaybornJoe G HollyfieldVera L BonilhaPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 7, Iss 6, p e38673 (2012)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Xiaorong Gu Nikolas J Neric John S Crabb John W Crabb Sanjoy K Bhattacharya Mary E Rayborn Joe G Hollyfield Vera L Bonilha Age-related changes in the retinal pigment epithelium (RPE).
description	<h4>Background</h4>Age-related changes in the retina are often accompanied by visual impairment but their mechanistic details remain poorly understood.<h4>Methodology</h4>Proteomic studies were pursued toward a better molecular understanding of retinal pigment epithelium (RPE) aging mechanisms. RPE cells were isolated from young adults (3-4 month-old) and old (24-25 month-old) F344BN rats, and separated into subcellular fractions containing apical microvilli (MV) and RPE cell bodies (CB) lacking their apical microvilli. Proteins were extracted in detergent, separated by SDS-PAGE, digested in situ with trypsin and analyzed by LC MS/MS. Select proteins detected in young and old rat RPE were further studied using immunofluorescence and Western blot analysis.<h4>Principal findings</h4>A total of 356 proteins were identified in RPE MV from young and 378 in RPE MV from old rats, 48% of which were common to each age group. A total of 897 proteins were identified in RPE CB from young rats and 675 in old CB, 56% of which were common to each age group. Several of the identified proteins, including proteins involved in response to oxidative stress, displayed both quantitative and qualitative changes in overall abundance during RPE aging. Numerous proteins were identified for the first time in the RPE. One such protein, collectrin, was localized to the apical membrane of apical brush border of proximal tubules where it likely regulates several amino acid transporters. Elsewhere, collectrin is involved in pancreatic β cell proliferation and insulin secretion. In the RPE, collectrin expression was significantly modulated during RPE aging. Another age-regulated, newly described protein was DJ-1, a protein extensively studied in brain where oxidative stress-related functions have been described.<h4>Conclusions/significance</h4>The data presented here reveals specific changes in the RPE during aging, providing the first protein database of RPE aging, which will facilitate future studies of age-related retinal diseases.
format	article
author	Xiaorong Gu Nikolas J Neric John S Crabb John W Crabb Sanjoy K Bhattacharya Mary E Rayborn Joe G Hollyfield Vera L Bonilha
author_facet	Xiaorong Gu Nikolas J Neric John S Crabb John W Crabb Sanjoy K Bhattacharya Mary E Rayborn Joe G Hollyfield Vera L Bonilha
author_sort	Xiaorong Gu
title	Age-related changes in the retinal pigment epithelium (RPE).
title_short	Age-related changes in the retinal pigment epithelium (RPE).
title_full	Age-related changes in the retinal pigment epithelium (RPE).
title_fullStr	Age-related changes in the retinal pigment epithelium (RPE).
title_full_unstemmed	Age-related changes in the retinal pigment epithelium (RPE).
title_sort	age-related changes in the retinal pigment epithelium (rpe).
publisher	Public Library of Science (PLoS)
publishDate	2012
url	https://doaj.org/article/b599f0f67e2a4faf83d2afc1cab678f1
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Age-related changes in the retinal pigment epithelium (RPE).

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