Human and mouse skeletal muscle stem cells: convergent and divergent mechanisms of myogenesis.

Satellite cells are the chief contributor to skeletal muscle growth and regeneration. The study of mouse satellite cells has accelerated in recent years due to technical advancements in the isolation of these cells. The study of human satellite cells has lagged and thus little is known about how the...

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Autores principales:	Akshay Bareja, Jason A Holt, Guizhen Luo, Calvin Chang, Junyu Lin, Aaron C Hinken, Johannes M Freudenberg, William E Kraus, William J Evans, Andrew N Billin
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2014
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/b59f032fb88a4b6cac5f3fde409dca6f
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spelling	oai:doaj.org-article:b59f032fb88a4b6cac5f3fde409dca6f2021-11-18T08:30:19ZHuman and mouse skeletal muscle stem cells: convergent and divergent mechanisms of myogenesis.1932-620310.1371/journal.pone.0090398https://doaj.org/article/b59f032fb88a4b6cac5f3fde409dca6f2014-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/24587351/pdf/?tool=EBIhttps://doaj.org/toc/1932-6203Satellite cells are the chief contributor to skeletal muscle growth and regeneration. The study of mouse satellite cells has accelerated in recent years due to technical advancements in the isolation of these cells. The study of human satellite cells has lagged and thus little is known about how the biology of mouse and human satellite cells compare. We developed a flow cytometry-based method to prospectively isolate human skeletal muscle progenitors from the satellite cell pool using positive and negative selection markers. Results show that this pool is enriched in PAX7 expressing cells that possess robust myogenic potential including the ability to give rise to de novo muscle in vivo. We compared mouse and human satellite cells in culture and identify differences in the elaboration of the myogenic genetic program and in the sensitivity of the cells to cytokine stimulation. These results indicate that not all mechanisms regulating mouse satellite cell activation are conserved in human satellite cells and that such differences may impact the clinical translation of therapeutics validated in mouse models. Thus, the findings of this study are relevant to developing therapies to combat muscle disease.Akshay BarejaJason A HoltGuizhen LuoCalvin ChangJunyu LinAaron C HinkenJohannes M FreudenbergWilliam E KrausWilliam J EvansAndrew N BillinPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 9, Iss 2, p e90398 (2014)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Akshay Bareja Jason A Holt Guizhen Luo Calvin Chang Junyu Lin Aaron C Hinken Johannes M Freudenberg William E Kraus William J Evans Andrew N Billin Human and mouse skeletal muscle stem cells: convergent and divergent mechanisms of myogenesis.
description	Satellite cells are the chief contributor to skeletal muscle growth and regeneration. The study of mouse satellite cells has accelerated in recent years due to technical advancements in the isolation of these cells. The study of human satellite cells has lagged and thus little is known about how the biology of mouse and human satellite cells compare. We developed a flow cytometry-based method to prospectively isolate human skeletal muscle progenitors from the satellite cell pool using positive and negative selection markers. Results show that this pool is enriched in PAX7 expressing cells that possess robust myogenic potential including the ability to give rise to de novo muscle in vivo. We compared mouse and human satellite cells in culture and identify differences in the elaboration of the myogenic genetic program and in the sensitivity of the cells to cytokine stimulation. These results indicate that not all mechanisms regulating mouse satellite cell activation are conserved in human satellite cells and that such differences may impact the clinical translation of therapeutics validated in mouse models. Thus, the findings of this study are relevant to developing therapies to combat muscle disease.
format	article
author	Akshay Bareja Jason A Holt Guizhen Luo Calvin Chang Junyu Lin Aaron C Hinken Johannes M Freudenberg William E Kraus William J Evans Andrew N Billin
author_facet	Akshay Bareja Jason A Holt Guizhen Luo Calvin Chang Junyu Lin Aaron C Hinken Johannes M Freudenberg William E Kraus William J Evans Andrew N Billin
author_sort	Akshay Bareja
title	Human and mouse skeletal muscle stem cells: convergent and divergent mechanisms of myogenesis.
title_short	Human and mouse skeletal muscle stem cells: convergent and divergent mechanisms of myogenesis.
title_full	Human and mouse skeletal muscle stem cells: convergent and divergent mechanisms of myogenesis.
title_fullStr	Human and mouse skeletal muscle stem cells: convergent and divergent mechanisms of myogenesis.
title_full_unstemmed	Human and mouse skeletal muscle stem cells: convergent and divergent mechanisms of myogenesis.
title_sort	human and mouse skeletal muscle stem cells: convergent and divergent mechanisms of myogenesis.
publisher	Public Library of Science (PLoS)
publishDate	2014
url	https://doaj.org/article/b59f032fb88a4b6cac5f3fde409dca6f
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Human and mouse skeletal muscle stem cells: convergent and divergent mechanisms of myogenesis.

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