Accelerated microfluidic native chemical ligation at difficult amino acids toward cyclic peptides

Flow-based peptide synthesis is a well-established method, yet difficult to combine with native chemical ligation (NCL), the go-to method for peptide cyclization. Here, the authors developed a microfluidic procedure for peptide cyclization within minutes, using NCL and an SEA alkylthioester peptide.

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Main Authors:	Nathalie Ollivier, Thomas Toupy, Ruben C. Hartkoorn, Rémi Desmet, Jean-Christophe M. Monbaliu, Oleg Melnyk
Format:	article
Language:	EN
Published:	Nature Portfolio 2018
Subjects:	Science Q
Online Access:	https://doaj.org/article/b5a06ec209fc4f7f99553330779adfca
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spelling	oai:doaj.org-article:b5a06ec209fc4f7f99553330779adfca2021-12-02T15:33:35ZAccelerated microfluidic native chemical ligation at difficult amino acids toward cyclic peptides10.1038/s41467-018-05264-82041-1723https://doaj.org/article/b5a06ec209fc4f7f99553330779adfca2018-07-01T00:00:00Zhttps://doi.org/10.1038/s41467-018-05264-8https://doaj.org/toc/2041-1723Flow-based peptide synthesis is a well-established method, yet difficult to combine with native chemical ligation (NCL), the go-to method for peptide cyclization. Here, the authors developed a microfluidic procedure for peptide cyclization within minutes, using NCL and an SEA alkylthioester peptide.Nathalie OllivierThomas ToupyRuben C. HartkoornRémi DesmetJean-Christophe M. MonbaliuOleg MelnykNature PortfolioarticleScienceQENNature Communications, Vol 9, Iss 1, Pp 1-12 (2018)
institution	DOAJ
collection	DOAJ
language	EN
topic	Science Q
spellingShingle	Science Q Nathalie Ollivier Thomas Toupy Ruben C. Hartkoorn Rémi Desmet Jean-Christophe M. Monbaliu Oleg Melnyk Accelerated microfluidic native chemical ligation at difficult amino acids toward cyclic peptides
description	Flow-based peptide synthesis is a well-established method, yet difficult to combine with native chemical ligation (NCL), the go-to method for peptide cyclization. Here, the authors developed a microfluidic procedure for peptide cyclization within minutes, using NCL and an SEA alkylthioester peptide.
format	article
author	Nathalie Ollivier Thomas Toupy Ruben C. Hartkoorn Rémi Desmet Jean-Christophe M. Monbaliu Oleg Melnyk
author_facet	Nathalie Ollivier Thomas Toupy Ruben C. Hartkoorn Rémi Desmet Jean-Christophe M. Monbaliu Oleg Melnyk
author_sort	Nathalie Ollivier
title	Accelerated microfluidic native chemical ligation at difficult amino acids toward cyclic peptides
title_short	Accelerated microfluidic native chemical ligation at difficult amino acids toward cyclic peptides
title_full	Accelerated microfluidic native chemical ligation at difficult amino acids toward cyclic peptides
title_fullStr	Accelerated microfluidic native chemical ligation at difficult amino acids toward cyclic peptides
title_full_unstemmed	Accelerated microfluidic native chemical ligation at difficult amino acids toward cyclic peptides
title_sort	accelerated microfluidic native chemical ligation at difficult amino acids toward cyclic peptides
publisher	Nature Portfolio
publishDate	2018
url	https://doaj.org/article/b5a06ec209fc4f7f99553330779adfca
work_keys_str_mv	AT nathalieollivier acceleratedmicrofluidicnativechemicalligationatdifficultaminoacidstowardcyclicpeptides AT thomastoupy acceleratedmicrofluidicnativechemicalligationatdifficultaminoacidstowardcyclicpeptides AT rubenchartkoorn acceleratedmicrofluidicnativechemicalligationatdifficultaminoacidstowardcyclicpeptides AT remidesmet acceleratedmicrofluidicnativechemicalligationatdifficultaminoacidstowardcyclicpeptides AT jeanchristophemmonbaliu acceleratedmicrofluidicnativechemicalligationatdifficultaminoacidstowardcyclicpeptides AT olegmelnyk acceleratedmicrofluidicnativechemicalligationatdifficultaminoacidstowardcyclicpeptides
_version_	1718387102167597056

Accelerated microfluidic native chemical ligation at difficult amino acids toward cyclic peptides

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