Accelerated microfluidic native chemical ligation at difficult amino acids toward cyclic peptides

Flow-based peptide synthesis is a well-established method, yet difficult to combine with native chemical ligation (NCL), the go-to method for peptide cyclization. Here, the authors developed a microfluidic procedure for peptide cyclization within minutes, using NCL and an SEA alkylthioester peptide.

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Autores principales: Nathalie Ollivier, Thomas Toupy, Ruben C. Hartkoorn, Rémi Desmet, Jean-Christophe M. Monbaliu, Oleg Melnyk
Formato: article
Lenguaje:EN
Publicado: Nature Portfolio 2018
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Acceso en línea:https://doaj.org/article/b5a06ec209fc4f7f99553330779adfca
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spelling oai:doaj.org-article:b5a06ec209fc4f7f99553330779adfca2021-12-02T15:33:35ZAccelerated microfluidic native chemical ligation at difficult amino acids toward cyclic peptides10.1038/s41467-018-05264-82041-1723https://doaj.org/article/b5a06ec209fc4f7f99553330779adfca2018-07-01T00:00:00Zhttps://doi.org/10.1038/s41467-018-05264-8https://doaj.org/toc/2041-1723Flow-based peptide synthesis is a well-established method, yet difficult to combine with native chemical ligation (NCL), the go-to method for peptide cyclization. Here, the authors developed a microfluidic procedure for peptide cyclization within minutes, using NCL and an SEA alkylthioester peptide.Nathalie OllivierThomas ToupyRuben C. HartkoornRémi DesmetJean-Christophe M. MonbaliuOleg MelnykNature PortfolioarticleScienceQENNature Communications, Vol 9, Iss 1, Pp 1-12 (2018)
institution DOAJ
collection DOAJ
language EN
topic Science
Q
spellingShingle Science
Q
Nathalie Ollivier
Thomas Toupy
Ruben C. Hartkoorn
Rémi Desmet
Jean-Christophe M. Monbaliu
Oleg Melnyk
Accelerated microfluidic native chemical ligation at difficult amino acids toward cyclic peptides
description Flow-based peptide synthesis is a well-established method, yet difficult to combine with native chemical ligation (NCL), the go-to method for peptide cyclization. Here, the authors developed a microfluidic procedure for peptide cyclization within minutes, using NCL and an SEA alkylthioester peptide.
format article
author Nathalie Ollivier
Thomas Toupy
Ruben C. Hartkoorn
Rémi Desmet
Jean-Christophe M. Monbaliu
Oleg Melnyk
author_facet Nathalie Ollivier
Thomas Toupy
Ruben C. Hartkoorn
Rémi Desmet
Jean-Christophe M. Monbaliu
Oleg Melnyk
author_sort Nathalie Ollivier
title Accelerated microfluidic native chemical ligation at difficult amino acids toward cyclic peptides
title_short Accelerated microfluidic native chemical ligation at difficult amino acids toward cyclic peptides
title_full Accelerated microfluidic native chemical ligation at difficult amino acids toward cyclic peptides
title_fullStr Accelerated microfluidic native chemical ligation at difficult amino acids toward cyclic peptides
title_full_unstemmed Accelerated microfluidic native chemical ligation at difficult amino acids toward cyclic peptides
title_sort accelerated microfluidic native chemical ligation at difficult amino acids toward cyclic peptides
publisher Nature Portfolio
publishDate 2018
url https://doaj.org/article/b5a06ec209fc4f7f99553330779adfca
work_keys_str_mv AT nathalieollivier acceleratedmicrofluidicnativechemicalligationatdifficultaminoacidstowardcyclicpeptides
AT thomastoupy acceleratedmicrofluidicnativechemicalligationatdifficultaminoacidstowardcyclicpeptides
AT rubenchartkoorn acceleratedmicrofluidicnativechemicalligationatdifficultaminoacidstowardcyclicpeptides
AT remidesmet acceleratedmicrofluidicnativechemicalligationatdifficultaminoacidstowardcyclicpeptides
AT jeanchristophemmonbaliu acceleratedmicrofluidicnativechemicalligationatdifficultaminoacidstowardcyclicpeptides
AT olegmelnyk acceleratedmicrofluidicnativechemicalligationatdifficultaminoacidstowardcyclicpeptides
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