Accelerated microfluidic native chemical ligation at difficult amino acids toward cyclic peptides
Flow-based peptide synthesis is a well-established method, yet difficult to combine with native chemical ligation (NCL), the go-to method for peptide cyclization. Here, the authors developed a microfluidic procedure for peptide cyclization within minutes, using NCL and an SEA alkylthioester peptide.
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Nature Portfolio
2018
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oai:doaj.org-article:b5a06ec209fc4f7f99553330779adfca2021-12-02T15:33:35ZAccelerated microfluidic native chemical ligation at difficult amino acids toward cyclic peptides10.1038/s41467-018-05264-82041-1723https://doaj.org/article/b5a06ec209fc4f7f99553330779adfca2018-07-01T00:00:00Zhttps://doi.org/10.1038/s41467-018-05264-8https://doaj.org/toc/2041-1723Flow-based peptide synthesis is a well-established method, yet difficult to combine with native chemical ligation (NCL), the go-to method for peptide cyclization. Here, the authors developed a microfluidic procedure for peptide cyclization within minutes, using NCL and an SEA alkylthioester peptide.Nathalie OllivierThomas ToupyRuben C. HartkoornRémi DesmetJean-Christophe M. MonbaliuOleg MelnykNature PortfolioarticleScienceQENNature Communications, Vol 9, Iss 1, Pp 1-12 (2018) |
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Science Q Nathalie Ollivier Thomas Toupy Ruben C. Hartkoorn Rémi Desmet Jean-Christophe M. Monbaliu Oleg Melnyk Accelerated microfluidic native chemical ligation at difficult amino acids toward cyclic peptides |
description |
Flow-based peptide synthesis is a well-established method, yet difficult to combine with native chemical ligation (NCL), the go-to method for peptide cyclization. Here, the authors developed a microfluidic procedure for peptide cyclization within minutes, using NCL and an SEA alkylthioester peptide. |
format |
article |
author |
Nathalie Ollivier Thomas Toupy Ruben C. Hartkoorn Rémi Desmet Jean-Christophe M. Monbaliu Oleg Melnyk |
author_facet |
Nathalie Ollivier Thomas Toupy Ruben C. Hartkoorn Rémi Desmet Jean-Christophe M. Monbaliu Oleg Melnyk |
author_sort |
Nathalie Ollivier |
title |
Accelerated microfluidic native chemical ligation at difficult amino acids toward cyclic peptides |
title_short |
Accelerated microfluidic native chemical ligation at difficult amino acids toward cyclic peptides |
title_full |
Accelerated microfluidic native chemical ligation at difficult amino acids toward cyclic peptides |
title_fullStr |
Accelerated microfluidic native chemical ligation at difficult amino acids toward cyclic peptides |
title_full_unstemmed |
Accelerated microfluidic native chemical ligation at difficult amino acids toward cyclic peptides |
title_sort |
accelerated microfluidic native chemical ligation at difficult amino acids toward cyclic peptides |
publisher |
Nature Portfolio |
publishDate |
2018 |
url |
https://doaj.org/article/b5a06ec209fc4f7f99553330779adfca |
work_keys_str_mv |
AT nathalieollivier acceleratedmicrofluidicnativechemicalligationatdifficultaminoacidstowardcyclicpeptides AT thomastoupy acceleratedmicrofluidicnativechemicalligationatdifficultaminoacidstowardcyclicpeptides AT rubenchartkoorn acceleratedmicrofluidicnativechemicalligationatdifficultaminoacidstowardcyclicpeptides AT remidesmet acceleratedmicrofluidicnativechemicalligationatdifficultaminoacidstowardcyclicpeptides AT jeanchristophemmonbaliu acceleratedmicrofluidicnativechemicalligationatdifficultaminoacidstowardcyclicpeptides AT olegmelnyk acceleratedmicrofluidicnativechemicalligationatdifficultaminoacidstowardcyclicpeptides |
_version_ |
1718387102167597056 |