Concentration-dependent effects of fullerenol on cultured hippocampal neuron viability

Ying-ying Zha,1 Bo Yang,1 Ming-liang Tang,2 Qiu-chen Guo,1 Ju-tao Chen,1 Long-ping Wen,3 Ming Wang11CAS Key Laboratory of Brain Function and Disease, School of Life Sciences, University of Science and Technology of China, Hefei, 2Suzhou Institute of NanoTech and NanoBionics, Chinese Academy of Scien...

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Autores principales:	Zha YY, Yang B, Tang ML, Guo QC, Chen JT, Wen LP, Wang M
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2012
Materias:	Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/b5a0daff7e7b43dba1fa0d90c0e86a52
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spelling	oai:doaj.org-article:b5a0daff7e7b43dba1fa0d90c0e86a522021-12-02T04:59:17ZConcentration-dependent effects of fullerenol on cultured hippocampal neuron viability1176-91141178-2013https://doaj.org/article/b5a0daff7e7b43dba1fa0d90c0e86a522012-06-01T00:00:00Zhttp://www.dovepress.com/concentration-dependent-effects-of-fullerenol-on-cultured-hippocampal--a10259https://doaj.org/toc/1176-9114https://doaj.org/toc/1178-2013Ying-ying Zha,1 Bo Yang,1 Ming-liang Tang,2 Qiu-chen Guo,1 Ju-tao Chen,1 Long-ping Wen,3 Ming Wang11CAS Key Laboratory of Brain Function and Disease, School of Life Sciences, University of Science and Technology of China, Hefei, 2Suzhou Institute of NanoTech and NanoBionics, Chinese Academy of Sciences, Suzhou, 3Laboratory of Nano-biology, School of Life Sciences, University of Science and Technology of China, Hefei, People&#39;s Republic of ChinaBackground: Recent studies have shown that the biological actions and toxicity of the water-soluble compound, polyhydroxyfullerene (fullerenol), are related to the concentrations present at a particular site of action. This study investigated the effects of different concentrations of fullerenol on cultured rat hippocampal neurons.Methods and results: Fullerenol at low concentrations significantly enhanced hippocampal neuron viability as tested by MTT assay and Hoechst 33342/propidium iodide double stain detection. At high concentrations, fullerenol induced apoptosis confirmed by Comet assay and assessment of caspase proteins.Conclusion: These findings suggest that fullerenol promotes cell death and protects against cell damage, depending on the concentration present. The concentration-dependent effects of fullerenol were mainly due to its influence on the reduction-oxidation pathway.Keywords: fullerenol, nanomaterial, neurotoxicity, neuroprotection, hippocampal neuronZha YYYang BTang MLGuo QCChen JTWen LPWang MDove Medical PressarticleMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol 2012, Iss default, Pp 3099-3109 (2012)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine (General) R5-920
spellingShingle	Medicine (General) R5-920 Zha YY Yang B Tang ML Guo QC Chen JT Wen LP Wang M Concentration-dependent effects of fullerenol on cultured hippocampal neuron viability
description	Ying-ying Zha,1 Bo Yang,1 Ming-liang Tang,2 Qiu-chen Guo,1 Ju-tao Chen,1 Long-ping Wen,3 Ming Wang11CAS Key Laboratory of Brain Function and Disease, School of Life Sciences, University of Science and Technology of China, Hefei, 2Suzhou Institute of NanoTech and NanoBionics, Chinese Academy of Sciences, Suzhou, 3Laboratory of Nano-biology, School of Life Sciences, University of Science and Technology of China, Hefei, People&#39;s Republic of ChinaBackground: Recent studies have shown that the biological actions and toxicity of the water-soluble compound, polyhydroxyfullerene (fullerenol), are related to the concentrations present at a particular site of action. This study investigated the effects of different concentrations of fullerenol on cultured rat hippocampal neurons.Methods and results: Fullerenol at low concentrations significantly enhanced hippocampal neuron viability as tested by MTT assay and Hoechst 33342/propidium iodide double stain detection. At high concentrations, fullerenol induced apoptosis confirmed by Comet assay and assessment of caspase proteins.Conclusion: These findings suggest that fullerenol promotes cell death and protects against cell damage, depending on the concentration present. The concentration-dependent effects of fullerenol were mainly due to its influence on the reduction-oxidation pathway.Keywords: fullerenol, nanomaterial, neurotoxicity, neuroprotection, hippocampal neuron
format	article
author	Zha YY Yang B Tang ML Guo QC Chen JT Wen LP Wang M
author_facet	Zha YY Yang B Tang ML Guo QC Chen JT Wen LP Wang M
author_sort	Zha YY
title	Concentration-dependent effects of fullerenol on cultured hippocampal neuron viability
title_short	Concentration-dependent effects of fullerenol on cultured hippocampal neuron viability
title_full	Concentration-dependent effects of fullerenol on cultured hippocampal neuron viability
title_fullStr	Concentration-dependent effects of fullerenol on cultured hippocampal neuron viability
title_full_unstemmed	Concentration-dependent effects of fullerenol on cultured hippocampal neuron viability
title_sort	concentration-dependent effects of fullerenol on cultured hippocampal neuron viability
publisher	Dove Medical Press
publishDate	2012
url	https://doaj.org/article/b5a0daff7e7b43dba1fa0d90c0e86a52
work_keys_str_mv	AT zhayy concentrationdependenteffectsoffullerenolonculturedhippocampalneuronviability AT yangb concentrationdependenteffectsoffullerenolonculturedhippocampalneuronviability AT tangml concentrationdependenteffectsoffullerenolonculturedhippocampalneuronviability AT guoqc concentrationdependenteffectsoffullerenolonculturedhippocampalneuronviability AT chenjt concentrationdependenteffectsoffullerenolonculturedhippocampalneuronviability AT wenlp concentrationdependenteffectsoffullerenolonculturedhippocampalneuronviability AT wangm concentrationdependenteffectsoffullerenolonculturedhippocampalneuronviability
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Concentration-dependent effects of fullerenol on cultured hippocampal neuron viability

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