The yeast osmostress response is carbon source dependent

Abstract Adaptation to altered osmotic conditions is a fundamental property of living cells and has been studied in detail in the yeast Saccharomyces cerevisiae. Yeast cells accumulate glycerol as compatible solute, controlled at different levels by the High Osmolarity Glycerol (HOG) response pathwa...

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Autores principales:	Roja Babazadeh, Petri-Jaan Lahtvee, Caroline B. Adiels, Mattias Goksör, Jens B. Nielsen, Stefan Hohmann
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/b5a0fb7b84ec4361b31950234ca77aa8
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spelling	oai:doaj.org-article:b5a0fb7b84ec4361b31950234ca77aa82021-12-02T16:06:29ZThe yeast osmostress response is carbon source dependent10.1038/s41598-017-01141-42045-2322https://doaj.org/article/b5a0fb7b84ec4361b31950234ca77aa82017-04-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-01141-4https://doaj.org/toc/2045-2322Abstract Adaptation to altered osmotic conditions is a fundamental property of living cells and has been studied in detail in the yeast Saccharomyces cerevisiae. Yeast cells accumulate glycerol as compatible solute, controlled at different levels by the High Osmolarity Glycerol (HOG) response pathway. Up to now, essentially all osmostress studies in yeast have been performed with glucose as carbon and energy source, which is metabolised by glycolysis with glycerol as a by-product. Here we investigated the response of yeast to osmotic stress when yeast is respiring ethanol as carbon and energy source. Remarkably, yeast cells do not accumulate glycerol under these conditions and it appears that trehalose may partly take over the role as compatible solute. The HOG pathway is activated in very much the same way as during growth on glucose and is also required for osmotic adaptation. Slower volume recovery was observed in ethanol-grown cells as compared to glucose-grown cells. Dependence on key regulators as well as the global gene expression profile were similar in many ways to those previously observed in glucose-grown cells. However, there are indications that cells re-arrange redox-metabolism when respiration is hampered under osmostress, a feature that could not be observed in glucose-grown cells.Roja BabazadehPetri-Jaan LahtveeCaroline B. AdielsMattias GoksörJens B. NielsenStefan HohmannNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-11 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Roja Babazadeh Petri-Jaan Lahtvee Caroline B. Adiels Mattias Goksör Jens B. Nielsen Stefan Hohmann The yeast osmostress response is carbon source dependent
description	Abstract Adaptation to altered osmotic conditions is a fundamental property of living cells and has been studied in detail in the yeast Saccharomyces cerevisiae. Yeast cells accumulate glycerol as compatible solute, controlled at different levels by the High Osmolarity Glycerol (HOG) response pathway. Up to now, essentially all osmostress studies in yeast have been performed with glucose as carbon and energy source, which is metabolised by glycolysis with glycerol as a by-product. Here we investigated the response of yeast to osmotic stress when yeast is respiring ethanol as carbon and energy source. Remarkably, yeast cells do not accumulate glycerol under these conditions and it appears that trehalose may partly take over the role as compatible solute. The HOG pathway is activated in very much the same way as during growth on glucose and is also required for osmotic adaptation. Slower volume recovery was observed in ethanol-grown cells as compared to glucose-grown cells. Dependence on key regulators as well as the global gene expression profile were similar in many ways to those previously observed in glucose-grown cells. However, there are indications that cells re-arrange redox-metabolism when respiration is hampered under osmostress, a feature that could not be observed in glucose-grown cells.
format	article
author	Roja Babazadeh Petri-Jaan Lahtvee Caroline B. Adiels Mattias Goksör Jens B. Nielsen Stefan Hohmann
author_facet	Roja Babazadeh Petri-Jaan Lahtvee Caroline B. Adiels Mattias Goksör Jens B. Nielsen Stefan Hohmann
author_sort	Roja Babazadeh
title	The yeast osmostress response is carbon source dependent
title_short	The yeast osmostress response is carbon source dependent
title_full	The yeast osmostress response is carbon source dependent
title_fullStr	The yeast osmostress response is carbon source dependent
title_full_unstemmed	The yeast osmostress response is carbon source dependent
title_sort	yeast osmostress response is carbon source dependent
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/b5a0fb7b84ec4361b31950234ca77aa8
work_keys_str_mv	AT rojababazadeh theyeastosmostressresponseiscarbonsourcedependent AT petrijaanlahtvee theyeastosmostressresponseiscarbonsourcedependent AT carolinebadiels theyeastosmostressresponseiscarbonsourcedependent AT mattiasgoksor theyeastosmostressresponseiscarbonsourcedependent AT jensbnielsen theyeastosmostressresponseiscarbonsourcedependent AT stefanhohmann theyeastosmostressresponseiscarbonsourcedependent AT rojababazadeh yeastosmostressresponseiscarbonsourcedependent AT petrijaanlahtvee yeastosmostressresponseiscarbonsourcedependent AT carolinebadiels yeastosmostressresponseiscarbonsourcedependent AT mattiasgoksor yeastosmostressresponseiscarbonsourcedependent AT jensbnielsen yeastosmostressresponseiscarbonsourcedependent AT stefanhohmann yeastosmostressresponseiscarbonsourcedependent
_version_	1718384984672174080

The yeast osmostress response is carbon source dependent

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