Metabolic Reconstruction and Modeling Microbial Electrosynthesis

Abstract Microbial electrosynthesis is a renewable energy and chemical production platform that relies on microbial cells to capture electrons from a cathode and fix carbon. Yet despite the promise of this technology, the metabolic capacity of the microbes that inhabit the electrode surface and cata...

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Autores principales:	Christopher W. Marshall, Daniel E. Ross, Kim M. Handley, Pamela B. Weisenhorn, Janaka N. Edirisinghe, Christopher S. Henry, Jack A. Gilbert, Harold D. May, R. Sean Norman
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/b5aa69297bd34f6abbce133970cea672
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spelling	oai:doaj.org-article:b5aa69297bd34f6abbce133970cea6722021-12-02T15:06:18ZMetabolic Reconstruction and Modeling Microbial Electrosynthesis10.1038/s41598-017-08877-z2045-2322https://doaj.org/article/b5aa69297bd34f6abbce133970cea6722017-08-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-08877-zhttps://doaj.org/toc/2045-2322Abstract Microbial electrosynthesis is a renewable energy and chemical production platform that relies on microbial cells to capture electrons from a cathode and fix carbon. Yet despite the promise of this technology, the metabolic capacity of the microbes that inhabit the electrode surface and catalyze electron transfer in these systems remains largely unknown. We assembled thirteen draft genomes from a microbial electrosynthesis system producing primarily acetate from carbon dioxide, and their transcriptional activity was mapped to genomes from cells on the electrode surface and in the supernatant. This allowed us to create a metabolic model of the predominant community members belonging to Acetobacterium, Sulfurospirillum, and Desulfovibrio. According to the model, the Acetobacterium was the primary carbon fixer, and a keystone member of the community. Transcripts of soluble hydrogenases and ferredoxins from Acetobacterium and hydrogenases, formate dehydrogenase, and cytochromes of Desulfovibrio were found in high abundance near the electrode surface. Cytochrome c oxidases of facultative members of the community were highly expressed in the supernatant despite completely sealed reactors and constant flushing with anaerobic gases. These molecular discoveries and metabolic modeling now serve as a foundation for future examination and development of electrosynthetic microbial communities.Christopher W. MarshallDaniel E. RossKim M. HandleyPamela B. WeisenhornJanaka N. EdirisingheChristopher S. HenryJack A. GilbertHarold D. MayR. Sean NormanNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-12 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Christopher W. Marshall Daniel E. Ross Kim M. Handley Pamela B. Weisenhorn Janaka N. Edirisinghe Christopher S. Henry Jack A. Gilbert Harold D. May R. Sean Norman Metabolic Reconstruction and Modeling Microbial Electrosynthesis
description	Abstract Microbial electrosynthesis is a renewable energy and chemical production platform that relies on microbial cells to capture electrons from a cathode and fix carbon. Yet despite the promise of this technology, the metabolic capacity of the microbes that inhabit the electrode surface and catalyze electron transfer in these systems remains largely unknown. We assembled thirteen draft genomes from a microbial electrosynthesis system producing primarily acetate from carbon dioxide, and their transcriptional activity was mapped to genomes from cells on the electrode surface and in the supernatant. This allowed us to create a metabolic model of the predominant community members belonging to Acetobacterium, Sulfurospirillum, and Desulfovibrio. According to the model, the Acetobacterium was the primary carbon fixer, and a keystone member of the community. Transcripts of soluble hydrogenases and ferredoxins from Acetobacterium and hydrogenases, formate dehydrogenase, and cytochromes of Desulfovibrio were found in high abundance near the electrode surface. Cytochrome c oxidases of facultative members of the community were highly expressed in the supernatant despite completely sealed reactors and constant flushing with anaerobic gases. These molecular discoveries and metabolic modeling now serve as a foundation for future examination and development of electrosynthetic microbial communities.
format	article
author	Christopher W. Marshall Daniel E. Ross Kim M. Handley Pamela B. Weisenhorn Janaka N. Edirisinghe Christopher S. Henry Jack A. Gilbert Harold D. May R. Sean Norman
author_facet	Christopher W. Marshall Daniel E. Ross Kim M. Handley Pamela B. Weisenhorn Janaka N. Edirisinghe Christopher S. Henry Jack A. Gilbert Harold D. May R. Sean Norman
author_sort	Christopher W. Marshall
title	Metabolic Reconstruction and Modeling Microbial Electrosynthesis
title_short	Metabolic Reconstruction and Modeling Microbial Electrosynthesis
title_full	Metabolic Reconstruction and Modeling Microbial Electrosynthesis
title_fullStr	Metabolic Reconstruction and Modeling Microbial Electrosynthesis
title_full_unstemmed	Metabolic Reconstruction and Modeling Microbial Electrosynthesis
title_sort	metabolic reconstruction and modeling microbial electrosynthesis
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/b5aa69297bd34f6abbce133970cea672
work_keys_str_mv	AT christopherwmarshall metabolicreconstructionandmodelingmicrobialelectrosynthesis AT danieleross metabolicreconstructionandmodelingmicrobialelectrosynthesis AT kimmhandley metabolicreconstructionandmodelingmicrobialelectrosynthesis AT pamelabweisenhorn metabolicreconstructionandmodelingmicrobialelectrosynthesis AT janakanedirisinghe metabolicreconstructionandmodelingmicrobialelectrosynthesis AT christophershenry metabolicreconstructionandmodelingmicrobialelectrosynthesis AT jackagilbert metabolicreconstructionandmodelingmicrobialelectrosynthesis AT harolddmay metabolicreconstructionandmodelingmicrobialelectrosynthesis AT rseannorman metabolicreconstructionandmodelingmicrobialelectrosynthesis
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Metabolic Reconstruction and Modeling Microbial Electrosynthesis

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