Portable microfluidic chip for detection of Escherichia coli in produce and blood

ShuQi Wang,1* Fatih Inci,1* Tafadzwa L Chaunzwa,1 Ajay Ramanujam,1 Aishwarya Vasudevan,1 Sathya Subramanian,1 Alexander Chi Fai Ip,1 Banupriya Sridharan,1 Umut Atakan Gurkan,1 Utkan Demirci,1,21Bio-Acoustic-MEMS in Medicine (BAMM) Laboratory, Center for Biomedical Engineering, Department of Medicine...

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Autores principales:	Wang S, Inci F, Chaunzwa TL, Ramanujam A, Vasudevan A, Subramanian S, Ip AC, Sridharan B, Gurkan UA, Demirci U
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2012
Materias:	Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/b5abc97a68fa4293bf7494bdb8ea3390
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spelling	oai:doaj.org-article:b5abc97a68fa4293bf7494bdb8ea33902021-12-02T01:48:46ZPortable microfluidic chip for detection of Escherichia coli in produce and blood1176-91141178-2013https://doaj.org/article/b5abc97a68fa4293bf7494bdb8ea33902012-05-01T00:00:00Zhttp://www.dovepress.com/portable-microfluidic-chip-for-detection-of-escherichia-coli-in-produc-a9994https://doaj.org/toc/1176-9114https://doaj.org/toc/1178-2013ShuQi Wang,1* Fatih Inci,1* Tafadzwa L Chaunzwa,1 Ajay Ramanujam,1 Aishwarya Vasudevan,1 Sathya Subramanian,1 Alexander Chi Fai Ip,1 Banupriya Sridharan,1 Umut Atakan Gurkan,1 Utkan Demirci,1,21Bio-Acoustic-MEMS in Medicine (BAMM) Laboratory, Center for Biomedical Engineering, Department of Medicine, Brigham and Women&#39;s Hospital, Harvard Medical School, Boston, MA 02139, 2Harvard-MIT Health Sciences and Technology, Cambridge, MA, USA, *These authors contributed equally to this workAbstract: Pathogenic agents can lead to severe clinical outcomes such as food poisoning, infection of open wounds, particularly in burn injuries and sepsis. Rapid detection of these pathogens can monitor these infections in a timely manner improving clinical outcomes. Conventional bacterial detection methods, such as agar plate culture or polymerase chain reaction, are time-consuming and dependent on complex and expensive instruments, which are not suitable for point-of-care (POC) settings. Therefore, there is an unmet need to develop a simple, rapid method for detection of pathogens such as Escherichia coli. Here, we present an immunobased microchip technology that can rapidly detect and quantify bacterial presence in various sources including physiologically relevant buffer solution (phosphate buffered saline [PBS]), blood, milk, and spinach. The microchip showed reliable capture of E. coli in PBS with an efficiency of 71.8% &plusmn; 5% at concentrations ranging from 50 to 4,000 CFUs/mL via lipopolysaccharide binding protein. The limits of detection of the microchip for PBS, blood, milk, and spinach samples were 50, 50, 50, and 500 CFUs/mL, respectively. The presented technology can be broadly applied to other pathogens at the POC, enabling various applications including surveillance of food supply and monitoring of bacteriology in patients with burn wounds.Keywords: Escherichia coli, microchip, sepsis, food safety, point-of-careWang SInci FChaunzwa TLRamanujam AVasudevan ASubramanian SIp ACSridharan BGurkan UADemirci UDove Medical PressarticleMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol 2012, Iss default, Pp 2591-2600 (2012)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine (General) R5-920
spellingShingle	Medicine (General) R5-920 Wang S Inci F Chaunzwa TL Ramanujam A Vasudevan A Subramanian S Ip AC Sridharan B Gurkan UA Demirci U Portable microfluidic chip for detection of Escherichia coli in produce and blood
description	ShuQi Wang,1* Fatih Inci,1* Tafadzwa L Chaunzwa,1 Ajay Ramanujam,1 Aishwarya Vasudevan,1 Sathya Subramanian,1 Alexander Chi Fai Ip,1 Banupriya Sridharan,1 Umut Atakan Gurkan,1 Utkan Demirci,1,21Bio-Acoustic-MEMS in Medicine (BAMM) Laboratory, Center for Biomedical Engineering, Department of Medicine, Brigham and Women&#39;s Hospital, Harvard Medical School, Boston, MA 02139, 2Harvard-MIT Health Sciences and Technology, Cambridge, MA, USA, *These authors contributed equally to this workAbstract: Pathogenic agents can lead to severe clinical outcomes such as food poisoning, infection of open wounds, particularly in burn injuries and sepsis. Rapid detection of these pathogens can monitor these infections in a timely manner improving clinical outcomes. Conventional bacterial detection methods, such as agar plate culture or polymerase chain reaction, are time-consuming and dependent on complex and expensive instruments, which are not suitable for point-of-care (POC) settings. Therefore, there is an unmet need to develop a simple, rapid method for detection of pathogens such as Escherichia coli. Here, we present an immunobased microchip technology that can rapidly detect and quantify bacterial presence in various sources including physiologically relevant buffer solution (phosphate buffered saline [PBS]), blood, milk, and spinach. The microchip showed reliable capture of E. coli in PBS with an efficiency of 71.8% &plusmn; 5% at concentrations ranging from 50 to 4,000 CFUs/mL via lipopolysaccharide binding protein. The limits of detection of the microchip for PBS, blood, milk, and spinach samples were 50, 50, 50, and 500 CFUs/mL, respectively. The presented technology can be broadly applied to other pathogens at the POC, enabling various applications including surveillance of food supply and monitoring of bacteriology in patients with burn wounds.Keywords: Escherichia coli, microchip, sepsis, food safety, point-of-care
format	article
author	Wang S Inci F Chaunzwa TL Ramanujam A Vasudevan A Subramanian S Ip AC Sridharan B Gurkan UA Demirci U
author_facet	Wang S Inci F Chaunzwa TL Ramanujam A Vasudevan A Subramanian S Ip AC Sridharan B Gurkan UA Demirci U
author_sort	Wang S
title	Portable microfluidic chip for detection of Escherichia coli in produce and blood
title_short	Portable microfluidic chip for detection of Escherichia coli in produce and blood
title_full	Portable microfluidic chip for detection of Escherichia coli in produce and blood
title_fullStr	Portable microfluidic chip for detection of Escherichia coli in produce and blood
title_full_unstemmed	Portable microfluidic chip for detection of Escherichia coli in produce and blood
title_sort	portable microfluidic chip for detection of escherichia coli in produce and blood
publisher	Dove Medical Press
publishDate	2012
url	https://doaj.org/article/b5abc97a68fa4293bf7494bdb8ea3390
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Portable microfluidic chip for detection of Escherichia coli in produce and blood

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