Tailored generation of insulin producing cells from canine mesenchymal stem cells derived from bone marrow and adipose tissue

Abstract The trend of regenerative therapy for diabetes in human and veterinary practices has conceptually been proven according to the Edmonton protocol and animal models. Establishing an alternative insulin-producing cell (IPC) resource for further clinical application is a challenging task. This...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Watchareewan Rodprasert, Sirirat Nantavisai, Koranis Pathanachai, Prasit Pavasant, Thanaphum Osathanon, Chenphop Sawangmake
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/b5b1b7182f6f4709893ea53f8f245958
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:b5b1b7182f6f4709893ea53f8f245958
record_format	dspace
spelling	oai:doaj.org-article:b5b1b7182f6f4709893ea53f8f2459582021-12-02T17:52:32ZTailored generation of insulin producing cells from canine mesenchymal stem cells derived from bone marrow and adipose tissue10.1038/s41598-021-91774-32045-2322https://doaj.org/article/b5b1b7182f6f4709893ea53f8f2459582021-06-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-91774-3https://doaj.org/toc/2045-2322Abstract The trend of regenerative therapy for diabetes in human and veterinary practices has conceptually been proven according to the Edmonton protocol and animal models. Establishing an alternative insulin-producing cell (IPC) resource for further clinical application is a challenging task. This study investigated IPC generation from two practical canine mesenchymal stem cells (cMSCs), canine bone marrow-derived MSCs (cBM-MSCs) and canine adipose-derived MSCs (cAD-MSCs). The results illustrated that cBM-MSCs and cAD-MSCs contain distinct pancreatic differentiation potential and require the tailor-made induction protocols. The effective generation of cBM-MSC-derived IPCs needs the integration of genetic and microenvironment manipulation using a hanging-drop culture of PDX1-transfected cBM-MSCs under a three-step pancreatic induction protocol. However, this protocol is resource- and time-consuming. Another study on cAD-MSC-derived IPC generation found that IPC colonies could be obtained by a low attachment culture under the three-step induction protocol. Further, Notch signaling inhibition during pancreatic endoderm/progenitor induction yielded IPC colonies through the trend of glucose-responsive C-peptide secretion. Thus, this study showed that IPCs could be obtained from cBM-MSCs and cAD-MSCs through different induction techniques. Also, further signaling manipulation studies should be conducted to maximize the protocol’s efficiency.Watchareewan RodprasertSirirat NantavisaiKoranis PathanachaiPrasit PavasantThanaphum OsathanonChenphop SawangmakeNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-17 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Watchareewan Rodprasert Sirirat Nantavisai Koranis Pathanachai Prasit Pavasant Thanaphum Osathanon Chenphop Sawangmake Tailored generation of insulin producing cells from canine mesenchymal stem cells derived from bone marrow and adipose tissue
description	Abstract The trend of regenerative therapy for diabetes in human and veterinary practices has conceptually been proven according to the Edmonton protocol and animal models. Establishing an alternative insulin-producing cell (IPC) resource for further clinical application is a challenging task. This study investigated IPC generation from two practical canine mesenchymal stem cells (cMSCs), canine bone marrow-derived MSCs (cBM-MSCs) and canine adipose-derived MSCs (cAD-MSCs). The results illustrated that cBM-MSCs and cAD-MSCs contain distinct pancreatic differentiation potential and require the tailor-made induction protocols. The effective generation of cBM-MSC-derived IPCs needs the integration of genetic and microenvironment manipulation using a hanging-drop culture of PDX1-transfected cBM-MSCs under a three-step pancreatic induction protocol. However, this protocol is resource- and time-consuming. Another study on cAD-MSC-derived IPC generation found that IPC colonies could be obtained by a low attachment culture under the three-step induction protocol. Further, Notch signaling inhibition during pancreatic endoderm/progenitor induction yielded IPC colonies through the trend of glucose-responsive C-peptide secretion. Thus, this study showed that IPCs could be obtained from cBM-MSCs and cAD-MSCs through different induction techniques. Also, further signaling manipulation studies should be conducted to maximize the protocol’s efficiency.
format	article
author	Watchareewan Rodprasert Sirirat Nantavisai Koranis Pathanachai Prasit Pavasant Thanaphum Osathanon Chenphop Sawangmake
author_facet	Watchareewan Rodprasert Sirirat Nantavisai Koranis Pathanachai Prasit Pavasant Thanaphum Osathanon Chenphop Sawangmake
author_sort	Watchareewan Rodprasert
title	Tailored generation of insulin producing cells from canine mesenchymal stem cells derived from bone marrow and adipose tissue
title_short	Tailored generation of insulin producing cells from canine mesenchymal stem cells derived from bone marrow and adipose tissue
title_full	Tailored generation of insulin producing cells from canine mesenchymal stem cells derived from bone marrow and adipose tissue
title_fullStr	Tailored generation of insulin producing cells from canine mesenchymal stem cells derived from bone marrow and adipose tissue
title_full_unstemmed	Tailored generation of insulin producing cells from canine mesenchymal stem cells derived from bone marrow and adipose tissue
title_sort	tailored generation of insulin producing cells from canine mesenchymal stem cells derived from bone marrow and adipose tissue
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/b5b1b7182f6f4709893ea53f8f245958
work_keys_str_mv	AT watchareewanrodprasert tailoredgenerationofinsulinproducingcellsfromcaninemesenchymalstemcellsderivedfrombonemarrowandadiposetissue AT siriratnantavisai tailoredgenerationofinsulinproducingcellsfromcaninemesenchymalstemcellsderivedfrombonemarrowandadiposetissue AT koranispathanachai tailoredgenerationofinsulinproducingcellsfromcaninemesenchymalstemcellsderivedfrombonemarrowandadiposetissue AT prasitpavasant tailoredgenerationofinsulinproducingcellsfromcaninemesenchymalstemcellsderivedfrombonemarrowandadiposetissue AT thanaphumosathanon tailoredgenerationofinsulinproducingcellsfromcaninemesenchymalstemcellsderivedfrombonemarrowandadiposetissue AT chenphopsawangmake tailoredgenerationofinsulinproducingcellsfromcaninemesenchymalstemcellsderivedfrombonemarrowandadiposetissue
_version_	1718379190270558208

Tailored generation of insulin producing cells from canine mesenchymal stem cells derived from bone marrow and adipose tissue

Ejemplares similares