Enrichment of low abundance DNA/RNA by oligonucleotide-clicked iron oxide nanoparticles

Abstract Detection of low abundance target DNA/RNA for clinical or research purposes is challenging because the target sequences can be hidden under a large background of human genomic or non-human metagenomic sequences. We describe a probe-based capture method to enrich for target sequences with DN...

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Autores principales:	Fereshte Damavandi, Weiwei Wang, Wei-Zheng Shen, Sibel Cetinel, Tracy Jordan, Juan Jovel, Carlo Montemagno, Gane Ka-Shu Wong
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/b5b386be42e4437995b44b2715de5576
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spelling	oai:doaj.org-article:b5b386be42e4437995b44b2715de55762021-12-02T17:12:25ZEnrichment of low abundance DNA/RNA by oligonucleotide-clicked iron oxide nanoparticles10.1038/s41598-021-92376-92045-2322https://doaj.org/article/b5b386be42e4437995b44b2715de55762021-06-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-92376-9https://doaj.org/toc/2045-2322Abstract Detection of low abundance target DNA/RNA for clinical or research purposes is challenging because the target sequences can be hidden under a large background of human genomic or non-human metagenomic sequences. We describe a probe-based capture method to enrich for target sequences with DNA-clicked iron oxide nanoparticles. Our method was tested against commercial capture assays using streptavidin beads, on a set of probes derived from a common genotype of the hepatitis C virus. We showed that our method is more specific and sensitive, most likely due to the combination of an inert silica coating and a high density of DNA probes clicked to the nanoparticles. This facilitates target capture below the limits of detection for TaqMan qPCR, and we believe that this method has the potential to transform management of infectious diseases.Fereshte DamavandiWeiwei WangWei-Zheng ShenSibel CetinelTracy JordanJuan JovelCarlo MontemagnoGane Ka-Shu WongNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-10 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Fereshte Damavandi Weiwei Wang Wei-Zheng Shen Sibel Cetinel Tracy Jordan Juan Jovel Carlo Montemagno Gane Ka-Shu Wong Enrichment of low abundance DNA/RNA by oligonucleotide-clicked iron oxide nanoparticles
description	Abstract Detection of low abundance target DNA/RNA for clinical or research purposes is challenging because the target sequences can be hidden under a large background of human genomic or non-human metagenomic sequences. We describe a probe-based capture method to enrich for target sequences with DNA-clicked iron oxide nanoparticles. Our method was tested against commercial capture assays using streptavidin beads, on a set of probes derived from a common genotype of the hepatitis C virus. We showed that our method is more specific and sensitive, most likely due to the combination of an inert silica coating and a high density of DNA probes clicked to the nanoparticles. This facilitates target capture below the limits of detection for TaqMan qPCR, and we believe that this method has the potential to transform management of infectious diseases.
format	article
author	Fereshte Damavandi Weiwei Wang Wei-Zheng Shen Sibel Cetinel Tracy Jordan Juan Jovel Carlo Montemagno Gane Ka-Shu Wong
author_facet	Fereshte Damavandi Weiwei Wang Wei-Zheng Shen Sibel Cetinel Tracy Jordan Juan Jovel Carlo Montemagno Gane Ka-Shu Wong
author_sort	Fereshte Damavandi
title	Enrichment of low abundance DNA/RNA by oligonucleotide-clicked iron oxide nanoparticles
title_short	Enrichment of low abundance DNA/RNA by oligonucleotide-clicked iron oxide nanoparticles
title_full	Enrichment of low abundance DNA/RNA by oligonucleotide-clicked iron oxide nanoparticles
title_fullStr	Enrichment of low abundance DNA/RNA by oligonucleotide-clicked iron oxide nanoparticles
title_full_unstemmed	Enrichment of low abundance DNA/RNA by oligonucleotide-clicked iron oxide nanoparticles
title_sort	enrichment of low abundance dna/rna by oligonucleotide-clicked iron oxide nanoparticles
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/b5b386be42e4437995b44b2715de5576
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Enrichment of low abundance DNA/RNA by oligonucleotide-clicked iron oxide nanoparticles

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