Evaluation and SNP typing of DNA from ultraviolet-irradiated human bloodstains using TaqMan assay

Abstract When detecting DNA profiles from forensic materials, it is pivotal to know the extent of degradation and which DNA marker can be genotyped. Ultraviolet (UV) is one of the common external factors that causes DNA damage, through which, an attempt to reveal cardinal genetic information can be...

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Autores principales:	Jian Tie, Seisaku Uchigasaki, Eiji Isobe
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/b5b75b50004743c28df42669bd2b378c
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spelling	oai:doaj.org-article:b5b75b50004743c28df42669bd2b378c2021-12-02T18:03:47ZEvaluation and SNP typing of DNA from ultraviolet-irradiated human bloodstains using TaqMan assay10.1038/s41598-021-87313-92045-2322https://doaj.org/article/b5b75b50004743c28df42669bd2b378c2021-04-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-87313-9https://doaj.org/toc/2045-2322Abstract When detecting DNA profiles from forensic materials, it is pivotal to know the extent of degradation and which DNA marker can be genotyped. Ultraviolet (UV) is one of the common external factors that causes DNA damage, through which, an attempt to reveal cardinal genetic information can be made. In this study, after irradiation with three different UV wavelengths, UV-damaged DNA in the bloodstains was analyzed with long and short TaqMan assays using real-time PCR. In addition, both short tandem repeat (STR) profiles and single nucleotide polymorphisms (SNPs) from the damaged DNA at different stages of UV exposure were also assessed. With increasing in UV irradiation cycles, there was a delay of the amplification curves accompanied with a decrease in the DNA amounts collected. Despite the amplification of STR genotype was not altered after 75 cycles of UVC irradiation, all 12 SNP loci could still be detected. Furthermore, a short-assay line was detected in the absence of an amplification of the evaluation curve. The results indicate that, although the DNA template might not be useful and suitable for analysis of STR profile, this approach is of some values in detecting SNPs.Jian TieSeisaku UchigasakiEiji IsobeNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-7 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Jian Tie Seisaku Uchigasaki Eiji Isobe Evaluation and SNP typing of DNA from ultraviolet-irradiated human bloodstains using TaqMan assay
description	Abstract When detecting DNA profiles from forensic materials, it is pivotal to know the extent of degradation and which DNA marker can be genotyped. Ultraviolet (UV) is one of the common external factors that causes DNA damage, through which, an attempt to reveal cardinal genetic information can be made. In this study, after irradiation with three different UV wavelengths, UV-damaged DNA in the bloodstains was analyzed with long and short TaqMan assays using real-time PCR. In addition, both short tandem repeat (STR) profiles and single nucleotide polymorphisms (SNPs) from the damaged DNA at different stages of UV exposure were also assessed. With increasing in UV irradiation cycles, there was a delay of the amplification curves accompanied with a decrease in the DNA amounts collected. Despite the amplification of STR genotype was not altered after 75 cycles of UVC irradiation, all 12 SNP loci could still be detected. Furthermore, a short-assay line was detected in the absence of an amplification of the evaluation curve. The results indicate that, although the DNA template might not be useful and suitable for analysis of STR profile, this approach is of some values in detecting SNPs.
format	article
author	Jian Tie Seisaku Uchigasaki Eiji Isobe
author_facet	Jian Tie Seisaku Uchigasaki Eiji Isobe
author_sort	Jian Tie
title	Evaluation and SNP typing of DNA from ultraviolet-irradiated human bloodstains using TaqMan assay
title_short	Evaluation and SNP typing of DNA from ultraviolet-irradiated human bloodstains using TaqMan assay
title_full	Evaluation and SNP typing of DNA from ultraviolet-irradiated human bloodstains using TaqMan assay
title_fullStr	Evaluation and SNP typing of DNA from ultraviolet-irradiated human bloodstains using TaqMan assay
title_full_unstemmed	Evaluation and SNP typing of DNA from ultraviolet-irradiated human bloodstains using TaqMan assay
title_sort	evaluation and snp typing of dna from ultraviolet-irradiated human bloodstains using taqman assay
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/b5b75b50004743c28df42669bd2b378c
work_keys_str_mv	AT jiantie evaluationandsnptypingofdnafromultravioletirradiatedhumanbloodstainsusingtaqmanassay AT seisakuuchigasaki evaluationandsnptypingofdnafromultravioletirradiatedhumanbloodstainsusingtaqmanassay AT eijiisobe evaluationandsnptypingofdnafromultravioletirradiatedhumanbloodstainsusingtaqmanassay
_version_	1718378705464590336

Evaluation and SNP typing of DNA from ultraviolet-irradiated human bloodstains using TaqMan assay

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