Genome-wide analyses of light-regulated genes in Aspergillus nidulans reveal a complex interplay between different photoreceptors and novel photoreceptor functions.

Fungi sense light of different wavelengths using blue-, green-, and red-light photoreceptors. Blue light sensing requires the "white-collar" proteins with flavin as chromophore, and red light is sensed through phytochrome. Here we analyzed genome-wide gene expression changes caused by shor...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Zhenzhong Yu, Christian Streng, Ramon F Seibeld, Olumuyiwa A Igbalajobi, Kai Leister, Julian Ingelfinger, Reinhard Fischer
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2021
Materias:	Genetics QH426-470
Acceso en línea:	https://doaj.org/article/b5b8a49d65c34ab394b7f7a77159cd3a
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:b5b8a49d65c34ab394b7f7a77159cd3a
record_format	dspace
spelling	oai:doaj.org-article:b5b8a49d65c34ab394b7f7a77159cd3a2021-12-02T20:03:29ZGenome-wide analyses of light-regulated genes in Aspergillus nidulans reveal a complex interplay between different photoreceptors and novel photoreceptor functions.1553-73901553-740410.1371/journal.pgen.1009845https://doaj.org/article/b5b8a49d65c34ab394b7f7a77159cd3a2021-10-01T00:00:00Zhttps://doi.org/10.1371/journal.pgen.1009845https://doaj.org/toc/1553-7390https://doaj.org/toc/1553-7404Fungi sense light of different wavelengths using blue-, green-, and red-light photoreceptors. Blue light sensing requires the "white-collar" proteins with flavin as chromophore, and red light is sensed through phytochrome. Here we analyzed genome-wide gene expression changes caused by short-term, low-light intensity illumination with blue-, red- or far-red light in Aspergillus nidulans and found that more than 1100 genes were differentially regulated. The largest number of up- and downregulated genes depended on the phytochrome FphA and the attached HOG pathway. FphA and the white-collar orthologue LreA fulfill activating but also repressing functions under all light conditions and both appear to have roles in the dark. Additionally, we found about 100 genes, which are red-light induced in the absence of phytochrome, suggesting alternative red-light sensing systems. We also found blue-light induced genes in the absence of the blue-light receptor LreA. We present evidence that cryptochrome may be part of this regulatory cue, but that phytochrome is essential for the response. In addition to in vivo data showing that FphA is involved in blue-light sensing, we performed spectroscopy of purified phytochrome and show that it responds indeed to blue light.Zhenzhong YuChristian StrengRamon F SeibeldOlumuyiwa A IgbalajobiKai LeisterJulian IngelfingerReinhard FischerPublic Library of Science (PLoS)articleGeneticsQH426-470ENPLoS Genetics, Vol 17, Iss 10, p e1009845 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Genetics QH426-470
spellingShingle	Genetics QH426-470 Zhenzhong Yu Christian Streng Ramon F Seibeld Olumuyiwa A Igbalajobi Kai Leister Julian Ingelfinger Reinhard Fischer Genome-wide analyses of light-regulated genes in Aspergillus nidulans reveal a complex interplay between different photoreceptors and novel photoreceptor functions.
description	Fungi sense light of different wavelengths using blue-, green-, and red-light photoreceptors. Blue light sensing requires the "white-collar" proteins with flavin as chromophore, and red light is sensed through phytochrome. Here we analyzed genome-wide gene expression changes caused by short-term, low-light intensity illumination with blue-, red- or far-red light in Aspergillus nidulans and found that more than 1100 genes were differentially regulated. The largest number of up- and downregulated genes depended on the phytochrome FphA and the attached HOG pathway. FphA and the white-collar orthologue LreA fulfill activating but also repressing functions under all light conditions and both appear to have roles in the dark. Additionally, we found about 100 genes, which are red-light induced in the absence of phytochrome, suggesting alternative red-light sensing systems. We also found blue-light induced genes in the absence of the blue-light receptor LreA. We present evidence that cryptochrome may be part of this regulatory cue, but that phytochrome is essential for the response. In addition to in vivo data showing that FphA is involved in blue-light sensing, we performed spectroscopy of purified phytochrome and show that it responds indeed to blue light.
format	article
author	Zhenzhong Yu Christian Streng Ramon F Seibeld Olumuyiwa A Igbalajobi Kai Leister Julian Ingelfinger Reinhard Fischer
author_facet	Zhenzhong Yu Christian Streng Ramon F Seibeld Olumuyiwa A Igbalajobi Kai Leister Julian Ingelfinger Reinhard Fischer
author_sort	Zhenzhong Yu
title	Genome-wide analyses of light-regulated genes in Aspergillus nidulans reveal a complex interplay between different photoreceptors and novel photoreceptor functions.
title_short	Genome-wide analyses of light-regulated genes in Aspergillus nidulans reveal a complex interplay between different photoreceptors and novel photoreceptor functions.
title_full	Genome-wide analyses of light-regulated genes in Aspergillus nidulans reveal a complex interplay between different photoreceptors and novel photoreceptor functions.
title_fullStr	Genome-wide analyses of light-regulated genes in Aspergillus nidulans reveal a complex interplay between different photoreceptors and novel photoreceptor functions.
title_full_unstemmed	Genome-wide analyses of light-regulated genes in Aspergillus nidulans reveal a complex interplay between different photoreceptors and novel photoreceptor functions.
title_sort	genome-wide analyses of light-regulated genes in aspergillus nidulans reveal a complex interplay between different photoreceptors and novel photoreceptor functions.
publisher	Public Library of Science (PLoS)
publishDate	2021
url	https://doaj.org/article/b5b8a49d65c34ab394b7f7a77159cd3a
work_keys_str_mv	AT zhenzhongyu genomewideanalysesoflightregulatedgenesinaspergillusnidulansrevealacomplexinterplaybetweendifferentphotoreceptorsandnovelphotoreceptorfunctions AT christianstreng genomewideanalysesoflightregulatedgenesinaspergillusnidulansrevealacomplexinterplaybetweendifferentphotoreceptorsandnovelphotoreceptorfunctions AT ramonfseibeld genomewideanalysesoflightregulatedgenesinaspergillusnidulansrevealacomplexinterplaybetweendifferentphotoreceptorsandnovelphotoreceptorfunctions AT olumuyiwaaigbalajobi genomewideanalysesoflightregulatedgenesinaspergillusnidulansrevealacomplexinterplaybetweendifferentphotoreceptorsandnovelphotoreceptorfunctions AT kaileister genomewideanalysesoflightregulatedgenesinaspergillusnidulansrevealacomplexinterplaybetweendifferentphotoreceptorsandnovelphotoreceptorfunctions AT julianingelfinger genomewideanalysesoflightregulatedgenesinaspergillusnidulansrevealacomplexinterplaybetweendifferentphotoreceptorsandnovelphotoreceptorfunctions AT reinhardfischer genomewideanalysesoflightregulatedgenesinaspergillusnidulansrevealacomplexinterplaybetweendifferentphotoreceptorsandnovelphotoreceptorfunctions
_version_	1718375681324220416

Genome-wide analyses of light-regulated genes in Aspergillus nidulans reveal a complex interplay between different photoreceptors and novel photoreceptor functions.

Ejemplares similares