The impact of silver and chitosan nanoparticles on A. fumigatus isolates from Ras cheese and sputum of respiratory patients

This work was designed to study the incidence of Aspergillus fumigatus (A. fumigatus) in Ras cheese sold in different markets at Sohag city, Egypt and also in sputum samples of respiratory diseases patients admitted to Sohag University Hospital, as well as study the effect of silver (SNPs) and ch...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Alshaima Ahmed Hassanien, Eman M Shaker, walaa elsherif
Formato:	article
Lenguaje:	EN
Publicado:	South Valley University 2021
Materias:	ras cheese a. fumigatus respiratory patients silver nanoparticles chitosan nanoparticles scanning electron microscope Agriculture S Veterinary medicine SF600-1100
Acceso en línea:	https://doaj.org/article/b5c01e3a45fe4745896925240f5861a4
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:b5c01e3a45fe4745896925240f5861a4
record_format	dspace
spelling	oai:doaj.org-article:b5c01e3a45fe4745896925240f5861a42021-12-02T17:38:17ZThe impact of silver and chitosan nanoparticles on A. fumigatus isolates from Ras cheese and sputum of respiratory patients10.21608/SVU.2021.56157.10942535-18262535-1877https://doaj.org/article/b5c01e3a45fe4745896925240f5861a42021-03-01T00:00:00Zhttps://svu.journals.ekb.eg/article_159160.htmlhttps://doaj.org/toc/2535-1826https://doaj.org/toc/2535-1877This work was designed to study the incidence of Aspergillus fumigatus (A. fumigatus) in Ras cheese sold in different markets at Sohag city, Egypt and also in sputum samples of respiratory diseases patients admitted to Sohag University Hospital, as well as study the effect of silver (SNPs) and chitosan (CNPs) nanoparticles against the A. fumigatus isolates. Potato dextrose agar (PDA) was used for mycological culture of samples and the antifungal effect of nanoparticles (with different concentrations) using well diffusion method. High resolution transmission electron microscope (HRTEM) was used for detection of nanoparticles size, and scanning electron microscope (SEM) was used for investigation of nanoparticles effect on A.fumigatus morphology. The isolation rate of A. fumigatus was 22.11 % (21/95), 32.17 % (37/115) from Ras cheese and sputum samples, respectively. SNPs had inhibitory effect at concentrations 1 and 2 µg/ml, and complete growth inhibition with concentrations 4, 8, and 16 µg/ ml. While CNPs had no effect in concentrations 1 and 2 µg/ ml, but at concentrations 4, 8 and 16 µg/ ml represented inhibitory effect with mean inhibition zones 5±0.21, 9±0.41 mm and 11 ±0.77mm, respectively. SNPs and CNPs by SEM patently damaged the A. fumigatus cell structure. Using nanoparticles against A. fumigatus from food and human sources is important to stop food spoilage and protect human health. So, further studies are necessary for the investigation of suitable application methods, and the expected hazards which may occur after nanoparticles usage.Alshaima Ahmed Hassanien Eman M Shakerwalaa elsherifSouth Valley Universityarticleras cheesea. fumigatusrespiratory patientssilver nanoparticleschitosan nanoparticlesscanning electron microscopeAgricultureSVeterinary medicineSF600-1100ENSVU-International Journal of Veterinary Sciences, Vol 4, Iss 1, Pp 79-86 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	ras cheese a. fumigatus respiratory patients silver nanoparticles chitosan nanoparticles scanning electron microscope Agriculture S Veterinary medicine SF600-1100
spellingShingle	ras cheese a. fumigatus respiratory patients silver nanoparticles chitosan nanoparticles scanning electron microscope Agriculture S Veterinary medicine SF600-1100 Alshaima Ahmed Hassanien Eman M Shaker walaa elsherif The impact of silver and chitosan nanoparticles on A. fumigatus isolates from Ras cheese and sputum of respiratory patients
description	This work was designed to study the incidence of Aspergillus fumigatus (A. fumigatus) in Ras cheese sold in different markets at Sohag city, Egypt and also in sputum samples of respiratory diseases patients admitted to Sohag University Hospital, as well as study the effect of silver (SNPs) and chitosan (CNPs) nanoparticles against the A. fumigatus isolates. Potato dextrose agar (PDA) was used for mycological culture of samples and the antifungal effect of nanoparticles (with different concentrations) using well diffusion method. High resolution transmission electron microscope (HRTEM) was used for detection of nanoparticles size, and scanning electron microscope (SEM) was used for investigation of nanoparticles effect on A.fumigatus morphology. The isolation rate of A. fumigatus was 22.11 % (21/95), 32.17 % (37/115) from Ras cheese and sputum samples, respectively. SNPs had inhibitory effect at concentrations 1 and 2 µg/ml, and complete growth inhibition with concentrations 4, 8, and 16 µg/ ml. While CNPs had no effect in concentrations 1 and 2 µg/ ml, but at concentrations 4, 8 and 16 µg/ ml represented inhibitory effect with mean inhibition zones 5±0.21, 9±0.41 mm and 11 ±0.77mm, respectively. SNPs and CNPs by SEM patently damaged the A. fumigatus cell structure. Using nanoparticles against A. fumigatus from food and human sources is important to stop food spoilage and protect human health. So, further studies are necessary for the investigation of suitable application methods, and the expected hazards which may occur after nanoparticles usage.
format	article
author	Alshaima Ahmed Hassanien Eman M Shaker walaa elsherif
author_facet	Alshaima Ahmed Hassanien Eman M Shaker walaa elsherif
author_sort	Alshaima Ahmed Hassanien
title	The impact of silver and chitosan nanoparticles on A. fumigatus isolates from Ras cheese and sputum of respiratory patients
title_short	The impact of silver and chitosan nanoparticles on A. fumigatus isolates from Ras cheese and sputum of respiratory patients
title_full	The impact of silver and chitosan nanoparticles on A. fumigatus isolates from Ras cheese and sputum of respiratory patients
title_fullStr	The impact of silver and chitosan nanoparticles on A. fumigatus isolates from Ras cheese and sputum of respiratory patients
title_full_unstemmed	The impact of silver and chitosan nanoparticles on A. fumigatus isolates from Ras cheese and sputum of respiratory patients
title_sort	impact of silver and chitosan nanoparticles on a. fumigatus isolates from ras cheese and sputum of respiratory patients
publisher	South Valley University
publishDate	2021
url	https://doaj.org/article/b5c01e3a45fe4745896925240f5861a4
work_keys_str_mv	AT alshaimaahmedhassanien theimpactofsilverandchitosannanoparticlesonafumigatusisolatesfromrascheeseandsputumofrespiratorypatients AT emanmshaker theimpactofsilverandchitosannanoparticlesonafumigatusisolatesfromrascheeseandsputumofrespiratorypatients AT walaaelsherif theimpactofsilverandchitosannanoparticlesonafumigatusisolatesfromrascheeseandsputumofrespiratorypatients AT alshaimaahmedhassanien impactofsilverandchitosannanoparticlesonafumigatusisolatesfromrascheeseandsputumofrespiratorypatients AT emanmshaker impactofsilverandchitosannanoparticlesonafumigatusisolatesfromrascheeseandsputumofrespiratorypatients AT walaaelsherif impactofsilverandchitosannanoparticlesonafumigatusisolatesfromrascheeseandsputumofrespiratorypatients
_version_	1718379843262873600

The impact of silver and chitosan nanoparticles on A. fumigatus isolates from Ras cheese and sputum of respiratory patients

Ejemplares similares