Innate And Adaptive Immunity are Progressively Activated in Parallel with Renal Injury in the 5/6 Renal Ablation Model

Abstract The mechanisms triggering renal inflammation in chronic kidney disease (CKD) are unclear. We performed a detailed analysis of the time course of innate and adaptive immunity activation in the 5/6 renal ablation (Nx) model. Munich-Wistar rats undergoing Nx were studied 15, 60 and 120 days af...

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Autores principales:	Camilla Fanelli, Simone C. A. Arias, Flavia G. Machado, Jessica K. Okuma, Denise M. A. C. Malheiros, Hatylas Azevedo, Carlos A. Moreira-Filho, Niels O. S. Camara, Clarice K. Fujihara, Roberto Zatz
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/b5c021dc62174d1c8eff25e163a2c255
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spelling	oai:doaj.org-article:b5c021dc62174d1c8eff25e163a2c2552021-12-02T11:53:09ZInnate And Adaptive Immunity are Progressively Activated in Parallel with Renal Injury in the 5/6 Renal Ablation Model10.1038/s41598-017-02915-62045-2322https://doaj.org/article/b5c021dc62174d1c8eff25e163a2c2552017-06-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-02915-6https://doaj.org/toc/2045-2322Abstract The mechanisms triggering renal inflammation in chronic kidney disease (CKD) are unclear. We performed a detailed analysis of the time course of innate and adaptive immunity activation in the 5/6 renal ablation (Nx) model. Munich-Wistar rats undergoing Nx were studied 15, 60 and 120 days after ablation. Hypertension, albuminuria, creatinine retention, interstitial expansion and infiltration by macrophages and T-lymphocytes were already evident 15 days after Nx. PCR-array was used to screen for altered gene expression, whereas gene and protein expressions of TLR4, CASP1, IL-1β and NLRP3 were individually assessed. Tlr4, Tlr5, Lbp, Nlrp3, Casp1, Irf7 and Il1b were already upregulated 15 days after Nx, while activation of Tlr2, Tlr7, Tlr9, Nod2, Tnf and Il6 was seen after 60 days post-ablation. The number of genes related to innate or adaptive immunity grew steadily with time. These observations indicate that parallel activation of innate and adaptive immunity antecedes glomerular injury and involves a growing number of intricate signaling pathways, helping to explain the difficulty in detaining renal injury in Nx as CKD advances, and, stressing the need for early treatment. Additionally, these findings may contribute to the search of therapeutic targets specific for advanced phases of CKD.Camilla FanelliSimone C. A. AriasFlavia G. MachadoJessica K. OkumaDenise M. A. C. MalheirosHatylas AzevedoCarlos A. Moreira-FilhoNiels O. S. CamaraClarice K. FujiharaRoberto ZatzNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-15 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Camilla Fanelli Simone C. A. Arias Flavia G. Machado Jessica K. Okuma Denise M. A. C. Malheiros Hatylas Azevedo Carlos A. Moreira-Filho Niels O. S. Camara Clarice K. Fujihara Roberto Zatz Innate And Adaptive Immunity are Progressively Activated in Parallel with Renal Injury in the 5/6 Renal Ablation Model
description	Abstract The mechanisms triggering renal inflammation in chronic kidney disease (CKD) are unclear. We performed a detailed analysis of the time course of innate and adaptive immunity activation in the 5/6 renal ablation (Nx) model. Munich-Wistar rats undergoing Nx were studied 15, 60 and 120 days after ablation. Hypertension, albuminuria, creatinine retention, interstitial expansion and infiltration by macrophages and T-lymphocytes were already evident 15 days after Nx. PCR-array was used to screen for altered gene expression, whereas gene and protein expressions of TLR4, CASP1, IL-1β and NLRP3 were individually assessed. Tlr4, Tlr5, Lbp, Nlrp3, Casp1, Irf7 and Il1b were already upregulated 15 days after Nx, while activation of Tlr2, Tlr7, Tlr9, Nod2, Tnf and Il6 was seen after 60 days post-ablation. The number of genes related to innate or adaptive immunity grew steadily with time. These observations indicate that parallel activation of innate and adaptive immunity antecedes glomerular injury and involves a growing number of intricate signaling pathways, helping to explain the difficulty in detaining renal injury in Nx as CKD advances, and, stressing the need for early treatment. Additionally, these findings may contribute to the search of therapeutic targets specific for advanced phases of CKD.
format	article
author	Camilla Fanelli Simone C. A. Arias Flavia G. Machado Jessica K. Okuma Denise M. A. C. Malheiros Hatylas Azevedo Carlos A. Moreira-Filho Niels O. S. Camara Clarice K. Fujihara Roberto Zatz
author_facet	Camilla Fanelli Simone C. A. Arias Flavia G. Machado Jessica K. Okuma Denise M. A. C. Malheiros Hatylas Azevedo Carlos A. Moreira-Filho Niels O. S. Camara Clarice K. Fujihara Roberto Zatz
author_sort	Camilla Fanelli
title	Innate And Adaptive Immunity are Progressively Activated in Parallel with Renal Injury in the 5/6 Renal Ablation Model
title_short	Innate And Adaptive Immunity are Progressively Activated in Parallel with Renal Injury in the 5/6 Renal Ablation Model
title_full	Innate And Adaptive Immunity are Progressively Activated in Parallel with Renal Injury in the 5/6 Renal Ablation Model
title_fullStr	Innate And Adaptive Immunity are Progressively Activated in Parallel with Renal Injury in the 5/6 Renal Ablation Model
title_full_unstemmed	Innate And Adaptive Immunity are Progressively Activated in Parallel with Renal Injury in the 5/6 Renal Ablation Model
title_sort	innate and adaptive immunity are progressively activated in parallel with renal injury in the 5/6 renal ablation model
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/b5c021dc62174d1c8eff25e163a2c255
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Innate And Adaptive Immunity are Progressively Activated in Parallel with Renal Injury in the 5/6 Renal Ablation Model

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