A GRFa2/Prop1/stem (GPS) cell niche in the pituitary.

<h4>Background</h4>The adult endocrine pituitary is known to host several hormone-producing cells regulating major physiological processes during life. Some candidates to progenitor/stem cells have been proposed. However, not much is known about pituitary cell renewal throughout life and...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Montse Garcia-Lavandeira, Víctor Quereda, Ignacio Flores, Carmen Saez, Esther Diaz-Rodriguez, Miguel A Japon, Aymee K Ryan, Maria A Blasco, Carlos Dieguez, Marcos Malumbres, Clara V Alvarez
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2009
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/b5d89240df2f44d3b791f8fdcf0097d8
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:b5d89240df2f44d3b791f8fdcf0097d8
record_format	dspace
spelling	oai:doaj.org-article:b5d89240df2f44d3b791f8fdcf0097d82021-11-25T06:16:43ZA GRFa2/Prop1/stem (GPS) cell niche in the pituitary.1932-620310.1371/journal.pone.0004815https://doaj.org/article/b5d89240df2f44d3b791f8fdcf0097d82009-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/19283075/?tool=EBIhttps://doaj.org/toc/1932-6203<h4>Background</h4>The adult endocrine pituitary is known to host several hormone-producing cells regulating major physiological processes during life. Some candidates to progenitor/stem cells have been proposed. However, not much is known about pituitary cell renewal throughout life and its homeostatic regulation during specific physiological changes, such as puberty or pregnancy, or in pathological conditions such as tumor development.<h4>Principal findings</h4>We have identified in rodents and humans a niche of non-endocrine cells characterized by the expression of GFRa2, a Ret co-receptor for Neurturin. These cells also express b-Catenin and E-cadherin in an oriented manner suggesting a planar polarity organization for the niche. In addition, cells in the niche uniquely express the pituitary-specific transcription factor Prop1, as well as known progenitor/stem markers such as Sox2, Sox9 and Oct4. Half of these GPS (GFRa2/Prop1/Stem) cells express S-100 whereas surrounding elongated cells in contact with GPS cells express Vimentin. GFRa2+-cells form non-endocrine spheroids in culture. These spheroids can be differentiated to hormone-producing cells or neurons outlining the neuroectoderm potential of these progenitors. In vivo, GPSs cells display slow proliferation after birth, retain BrdU label and show long telomeres in its nuclei, indicating progenitor/stem cell properties in vivo.<h4>Significance</h4>Our results suggest the presence in the adult pituitary of a specific niche of cells characterized by the expression of GFRa2, the pituitary-specific protein Prop1 and stem cell markers. These GPS cells are able to produce different hormone-producing and neuron-like cells and they may therefore contribute to postnatal pituitary homeostasis. Indeed, the relative abundance of GPS numbers is altered in Cdk4-deficient mice, a model of hypopituitarism induced by the lack of this cyclin-dependent kinase. Thus, GPS cells may display functional relevance in the physiological expansion of the pituitary gland throughout life as well as protection from pituitary disease.Montse Garcia-LavandeiraVíctor QueredaIgnacio FloresCarmen SaezEsther Diaz-RodriguezMiguel A JaponAymee K RyanMaria A BlascoCarlos DieguezMarcos MalumbresClara V AlvarezPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 4, Iss 3, p e4815 (2009)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Montse Garcia-Lavandeira Víctor Quereda Ignacio Flores Carmen Saez Esther Diaz-Rodriguez Miguel A Japon Aymee K Ryan Maria A Blasco Carlos Dieguez Marcos Malumbres Clara V Alvarez A GRFa2/Prop1/stem (GPS) cell niche in the pituitary.
description	<h4>Background</h4>The adult endocrine pituitary is known to host several hormone-producing cells regulating major physiological processes during life. Some candidates to progenitor/stem cells have been proposed. However, not much is known about pituitary cell renewal throughout life and its homeostatic regulation during specific physiological changes, such as puberty or pregnancy, or in pathological conditions such as tumor development.<h4>Principal findings</h4>We have identified in rodents and humans a niche of non-endocrine cells characterized by the expression of GFRa2, a Ret co-receptor for Neurturin. These cells also express b-Catenin and E-cadherin in an oriented manner suggesting a planar polarity organization for the niche. In addition, cells in the niche uniquely express the pituitary-specific transcription factor Prop1, as well as known progenitor/stem markers such as Sox2, Sox9 and Oct4. Half of these GPS (GFRa2/Prop1/Stem) cells express S-100 whereas surrounding elongated cells in contact with GPS cells express Vimentin. GFRa2+-cells form non-endocrine spheroids in culture. These spheroids can be differentiated to hormone-producing cells or neurons outlining the neuroectoderm potential of these progenitors. In vivo, GPSs cells display slow proliferation after birth, retain BrdU label and show long telomeres in its nuclei, indicating progenitor/stem cell properties in vivo.<h4>Significance</h4>Our results suggest the presence in the adult pituitary of a specific niche of cells characterized by the expression of GFRa2, the pituitary-specific protein Prop1 and stem cell markers. These GPS cells are able to produce different hormone-producing and neuron-like cells and they may therefore contribute to postnatal pituitary homeostasis. Indeed, the relative abundance of GPS numbers is altered in Cdk4-deficient mice, a model of hypopituitarism induced by the lack of this cyclin-dependent kinase. Thus, GPS cells may display functional relevance in the physiological expansion of the pituitary gland throughout life as well as protection from pituitary disease.
format	article
author	Montse Garcia-Lavandeira Víctor Quereda Ignacio Flores Carmen Saez Esther Diaz-Rodriguez Miguel A Japon Aymee K Ryan Maria A Blasco Carlos Dieguez Marcos Malumbres Clara V Alvarez
author_facet	Montse Garcia-Lavandeira Víctor Quereda Ignacio Flores Carmen Saez Esther Diaz-Rodriguez Miguel A Japon Aymee K Ryan Maria A Blasco Carlos Dieguez Marcos Malumbres Clara V Alvarez
author_sort	Montse Garcia-Lavandeira
title	A GRFa2/Prop1/stem (GPS) cell niche in the pituitary.
title_short	A GRFa2/Prop1/stem (GPS) cell niche in the pituitary.
title_full	A GRFa2/Prop1/stem (GPS) cell niche in the pituitary.
title_fullStr	A GRFa2/Prop1/stem (GPS) cell niche in the pituitary.
title_full_unstemmed	A GRFa2/Prop1/stem (GPS) cell niche in the pituitary.
title_sort	grfa2/prop1/stem (gps) cell niche in the pituitary.
publisher	Public Library of Science (PLoS)
publishDate	2009
url	https://doaj.org/article/b5d89240df2f44d3b791f8fdcf0097d8
work_keys_str_mv	AT montsegarcialavandeira agrfa2prop1stemgpscellnicheinthepituitary AT victorquereda agrfa2prop1stemgpscellnicheinthepituitary AT ignacioflores agrfa2prop1stemgpscellnicheinthepituitary AT carmensaez agrfa2prop1stemgpscellnicheinthepituitary AT estherdiazrodriguez agrfa2prop1stemgpscellnicheinthepituitary AT miguelajapon agrfa2prop1stemgpscellnicheinthepituitary AT aymeekryan agrfa2prop1stemgpscellnicheinthepituitary AT mariaablasco agrfa2prop1stemgpscellnicheinthepituitary AT carlosdieguez agrfa2prop1stemgpscellnicheinthepituitary AT marcosmalumbres agrfa2prop1stemgpscellnicheinthepituitary AT claravalvarez agrfa2prop1stemgpscellnicheinthepituitary AT montsegarcialavandeira grfa2prop1stemgpscellnicheinthepituitary AT victorquereda grfa2prop1stemgpscellnicheinthepituitary AT ignacioflores grfa2prop1stemgpscellnicheinthepituitary AT carmensaez grfa2prop1stemgpscellnicheinthepituitary AT estherdiazrodriguez grfa2prop1stemgpscellnicheinthepituitary AT miguelajapon grfa2prop1stemgpscellnicheinthepituitary AT aymeekryan grfa2prop1stemgpscellnicheinthepituitary AT mariaablasco grfa2prop1stemgpscellnicheinthepituitary AT carlosdieguez grfa2prop1stemgpscellnicheinthepituitary AT marcosmalumbres grfa2prop1stemgpscellnicheinthepituitary AT claravalvarez grfa2prop1stemgpscellnicheinthepituitary
_version_	1718414004096860160

A GRFa2/Prop1/stem (GPS) cell niche in the pituitary.

Ejemplares similares