Micropropagation of clonal lines of thorny artichoke (Cynara scolymus L.)

The aim of the study was to evaluate the in vitro propagation ability of 10 clonal lines of thorny globe artichoke (Cynara scolymus L.). The study methodology comprised five stages of evaluation. The stages evaluated were initiation, multiplication, rooting, acclimatization, and transplant to the fi...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	E. Catacora, J. Olivera, Z. Ramos, Z. Alve, R. Pinedo
Formato:	article
Lenguaje:	EN
Publicado:	Universidad Nacional Agraria La Molina 2019
Materias:	offspring clonal propagation rooting acclimatization thorny globe artichoke Agriculture (General) S1-972
Acceso en línea:	https://doaj.org/article/b5df2f6e49bd4e109e61d1f8f7f721b8
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:b5df2f6e49bd4e109e61d1f8f7f721b8
record_format	dspace
spelling	oai:doaj.org-article:b5df2f6e49bd4e109e61d1f8f7f721b82021-12-04T17:36:09ZMicropropagation of clonal lines of thorny artichoke (Cynara scolymus L.)2616-447710.21704/pja.v3i1.1280https://doaj.org/article/b5df2f6e49bd4e109e61d1f8f7f721b82019-04-01T00:00:00Zhttps://revistas.lamolina.edu.pe/index.php/jpagronomy/article/view/1280https://doaj.org/toc/2616-4477The aim of the study was to evaluate the in vitro propagation ability of 10 clonal lines of thorny globe artichoke (Cynara scolymus L.). The study methodology comprised five stages of evaluation. The stages evaluated were initiation, multiplication, rooting, acclimatization, and transplant to the field. The study began with the initiation of dissected shoot tips of 10 clonal lines in test tubes containing the Murashige and Skoog (MS) medium. Best results were obtained when explants were cultured on an induction medium containing MS + naphthalene acetic acid (NAA) 1.0 mg l−1 + benzyl aminopurine (BA) 1.0 mg l−1, highlighting clonal lines L-250, L-132, and L-62. Because of high rates of vitrification and phenolization in the initial stage, clonal lines L-24, L-127, and L-142 were discarded from the study. Therefore, only seven clonal lines were included for evaluation in the multiplication stage. Once the microplants were obtained under laboratory condition in the culture medium, they were immediately transferred to a proliferation medium containing MS + BA 1.0 mg l−1. Only in three clonal lines (L-132, L-200, and L-250), a high multiplication rate (3.5 shoots/explant) was achieved with axillary bud formation. Of the seven clonal lines evaluated, clonal line L-250 achieved the highest rates in the variables shoot height (3.38 cm), number of leaves (13.4), and number of shoots/explant (4.4). In the rooting stage, clonal line L-250 obtained a significant improvement by transferring plantlets to direct acclimatization after 20 days of in vitro root induction in a medium containing MS + NAA 1.0 mg l−1. Similarly, in the acclimatization stage, the clonal line L-250 showed a significant result. Then, in the transplantation stage, the plants were transplanted to the field with 100% rooting; 30 days after the transplantation, the clonal line L-250 obtained 100% survival in the field than the control treatments (offspring from two locations were used – Mito and Alayo). As the rooting period is reduced by approximately 20 days by inducing direct root formation under greenhouse conditions, the micropropagation technique is optimized with the protocol used in this study.E. CatacoraJ. OliveraZ. RamosZ. AlveR. PinedoUniversidad Nacional Agraria La Molinaarticleoffspringclonal propagationrootingacclimatizationthorny globe artichokeAgriculture (General)S1-972ENPeruvian Journal of Agronomy, Vol 3, Iss 1, Pp 29-38 (2019)
institution	DOAJ
collection	DOAJ
language	EN
topic	offspring clonal propagation rooting acclimatization thorny globe artichoke Agriculture (General) S1-972
spellingShingle	offspring clonal propagation rooting acclimatization thorny globe artichoke Agriculture (General) S1-972 E. Catacora J. Olivera Z. Ramos Z. Alve R. Pinedo Micropropagation of clonal lines of thorny artichoke (Cynara scolymus L.)
description	The aim of the study was to evaluate the in vitro propagation ability of 10 clonal lines of thorny globe artichoke (Cynara scolymus L.). The study methodology comprised five stages of evaluation. The stages evaluated were initiation, multiplication, rooting, acclimatization, and transplant to the field. The study began with the initiation of dissected shoot tips of 10 clonal lines in test tubes containing the Murashige and Skoog (MS) medium. Best results were obtained when explants were cultured on an induction medium containing MS + naphthalene acetic acid (NAA) 1.0 mg l−1 + benzyl aminopurine (BA) 1.0 mg l−1, highlighting clonal lines L-250, L-132, and L-62. Because of high rates of vitrification and phenolization in the initial stage, clonal lines L-24, L-127, and L-142 were discarded from the study. Therefore, only seven clonal lines were included for evaluation in the multiplication stage. Once the microplants were obtained under laboratory condition in the culture medium, they were immediately transferred to a proliferation medium containing MS + BA 1.0 mg l−1. Only in three clonal lines (L-132, L-200, and L-250), a high multiplication rate (3.5 shoots/explant) was achieved with axillary bud formation. Of the seven clonal lines evaluated, clonal line L-250 achieved the highest rates in the variables shoot height (3.38 cm), number of leaves (13.4), and number of shoots/explant (4.4). In the rooting stage, clonal line L-250 obtained a significant improvement by transferring plantlets to direct acclimatization after 20 days of in vitro root induction in a medium containing MS + NAA 1.0 mg l−1. Similarly, in the acclimatization stage, the clonal line L-250 showed a significant result. Then, in the transplantation stage, the plants were transplanted to the field with 100% rooting; 30 days after the transplantation, the clonal line L-250 obtained 100% survival in the field than the control treatments (offspring from two locations were used – Mito and Alayo). As the rooting period is reduced by approximately 20 days by inducing direct root formation under greenhouse conditions, the micropropagation technique is optimized with the protocol used in this study.
format	article
author	E. Catacora J. Olivera Z. Ramos Z. Alve R. Pinedo
author_facet	E. Catacora J. Olivera Z. Ramos Z. Alve R. Pinedo
author_sort	E. Catacora
title	Micropropagation of clonal lines of thorny artichoke (Cynara scolymus L.)
title_short	Micropropagation of clonal lines of thorny artichoke (Cynara scolymus L.)
title_full	Micropropagation of clonal lines of thorny artichoke (Cynara scolymus L.)
title_fullStr	Micropropagation of clonal lines of thorny artichoke (Cynara scolymus L.)
title_full_unstemmed	Micropropagation of clonal lines of thorny artichoke (Cynara scolymus L.)
title_sort	micropropagation of clonal lines of thorny artichoke (cynara scolymus l.)
publisher	Universidad Nacional Agraria La Molina
publishDate	2019
url	https://doaj.org/article/b5df2f6e49bd4e109e61d1f8f7f721b8
work_keys_str_mv	AT ecatacora micropropagationofclonallinesofthornyartichokecynarascolymusl AT jolivera micropropagationofclonallinesofthornyartichokecynarascolymusl AT zramos micropropagationofclonallinesofthornyartichokecynarascolymusl AT zalve micropropagationofclonallinesofthornyartichokecynarascolymusl AT rpinedo micropropagationofclonallinesofthornyartichokecynarascolymusl
_version_	1718372696643862528

Micropropagation of clonal lines of thorny artichoke (Cynara scolymus L.)

Ejemplares similares