Effect of modified surface and architectonics of porous-permeable TiNi-based alloy scaffold on cellular biocompatibility

Cellular scaffolds based on porous biomaterials play an important role in the engineering of various tissues. Our porous-permeable cellular TiNi-based alloy scaffolds combine the advantages of metal structures, hardness, wear resistance and elasticity similar to that of human tissues. The aim of the...

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Autores principales:	O. V. Kokorev, S. V. Gyunter, V. N. Khodorenko, G. T. Dambaev
Formato:	article
Lenguaje:	RU
Publicado:	Scientific Сentre for Family Health and Human Reproduction Problems 2018
Materias:	biomaterial biocompatibility scaffold porous tini-based alloy tissue engineering Science Q
Acceso en línea:	https://doaj.org/article/b5e5e29fdd914c06971151bca6a573b6
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spelling	oai:doaj.org-article:b5e5e29fdd914c06971151bca6a573b62021-11-23T06:14:40ZEffect of modified surface and architectonics of porous-permeable TiNi-based alloy scaffold on cellular biocompatibility2541-94202587-959610.29413/ABS.2018-3.3.29https://doaj.org/article/b5e5e29fdd914c06971151bca6a573b62018-05-01T00:00:00Zhttps://www.actabiomedica.ru/jour/article/view/606https://doaj.org/toc/2541-9420https://doaj.org/toc/2587-9596Cellular scaffolds based on porous biomaterials play an important role in the engineering of various tissues. Our porous-permeable cellular TiNi-based alloy scaffolds combine the advantages of metal structures, hardness, wear resistance and elasticity similar to that of human tissues. The aim of the research was the study on the change in the surface layer of samples from TiNi-based alloy and on the biotesting of modified samples with the 3H3 fibroblast line. Materials and methods: The biocompatibility of several samples of TiNi-based alloy scaffolds with an average pore size of 83,150,365 μm with modified (acid treatment) surface with 3H3 fibroblast line. Results. The porous-permeable cellular TiNi-based alloy scaffolds (average pore size = 150 μm) modified by treatment with solution of concentrated acids exhibited the highest biocompatibility with a fibroblast culture. It was shown that hemolysis of TiNi-based alloy samples, which are intact and treated with solution of concentrated acids, does not exceed 2 %. Direct cultivation of modified samples with fibroblast line in the cytotoxic test showed low cytotoxicity of the tested cells. The studies carried out using a scanning microscope showed that mesenchymal cells of bone marrow are attached in sufficient quantities to the microporous surface of the modified samples, which allows them to grow and proliferate in the pore space of TiNi-based alloy scaffolds and to cultivate tissue in vitro. Conclusion. Samples of scaffolds manufactured by the SHS-method and modified by treatment with concentrated solution of acids are technological and perspective biomaterial for their use as implantable clinically useful scaffolds.O. V. KokorevS. V. GyunterV. N. KhodorenkoG. T. DambaevScientific Сentre for Family Health and Human Reproduction Problemsarticlebiomaterialbiocompatibilityscaffoldporous tini-based alloytissue engineeringScienceQRUActa Biomedica Scientifica, Vol 3, Iss 3, Pp 188-194 (2018)
institution	DOAJ
collection	DOAJ
language	RU
topic	biomaterial biocompatibility scaffold porous tini-based alloy tissue engineering Science Q
spellingShingle	biomaterial biocompatibility scaffold porous tini-based alloy tissue engineering Science Q O. V. Kokorev S. V. Gyunter V. N. Khodorenko G. T. Dambaev Effect of modified surface and architectonics of porous-permeable TiNi-based alloy scaffold on cellular biocompatibility
description	Cellular scaffolds based on porous biomaterials play an important role in the engineering of various tissues. Our porous-permeable cellular TiNi-based alloy scaffolds combine the advantages of metal structures, hardness, wear resistance and elasticity similar to that of human tissues. The aim of the research was the study on the change in the surface layer of samples from TiNi-based alloy and on the biotesting of modified samples with the 3H3 fibroblast line. Materials and methods: The biocompatibility of several samples of TiNi-based alloy scaffolds with an average pore size of 83,150,365 μm with modified (acid treatment) surface with 3H3 fibroblast line. Results. The porous-permeable cellular TiNi-based alloy scaffolds (average pore size = 150 μm) modified by treatment with solution of concentrated acids exhibited the highest biocompatibility with a fibroblast culture. It was shown that hemolysis of TiNi-based alloy samples, which are intact and treated with solution of concentrated acids, does not exceed 2 %. Direct cultivation of modified samples with fibroblast line in the cytotoxic test showed low cytotoxicity of the tested cells. The studies carried out using a scanning microscope showed that mesenchymal cells of bone marrow are attached in sufficient quantities to the microporous surface of the modified samples, which allows them to grow and proliferate in the pore space of TiNi-based alloy scaffolds and to cultivate tissue in vitro. Conclusion. Samples of scaffolds manufactured by the SHS-method and modified by treatment with concentrated solution of acids are technological and perspective biomaterial for their use as implantable clinically useful scaffolds.
format	article
author	O. V. Kokorev S. V. Gyunter V. N. Khodorenko G. T. Dambaev
author_facet	O. V. Kokorev S. V. Gyunter V. N. Khodorenko G. T. Dambaev
author_sort	O. V. Kokorev
title	Effect of modified surface and architectonics of porous-permeable TiNi-based alloy scaffold on cellular biocompatibility
title_short	Effect of modified surface and architectonics of porous-permeable TiNi-based alloy scaffold on cellular biocompatibility
title_full	Effect of modified surface and architectonics of porous-permeable TiNi-based alloy scaffold on cellular biocompatibility
title_fullStr	Effect of modified surface and architectonics of porous-permeable TiNi-based alloy scaffold on cellular biocompatibility
title_full_unstemmed	Effect of modified surface and architectonics of porous-permeable TiNi-based alloy scaffold on cellular biocompatibility
title_sort	effect of modified surface and architectonics of porous-permeable tini-based alloy scaffold on cellular biocompatibility
publisher	Scientific Сentre for Family Health and Human Reproduction Problems
publishDate	2018
url	https://doaj.org/article/b5e5e29fdd914c06971151bca6a573b6
work_keys_str_mv	AT ovkokorev effectofmodifiedsurfaceandarchitectonicsofporouspermeabletinibasedalloyscaffoldoncellularbiocompatibility AT svgyunter effectofmodifiedsurfaceandarchitectonicsofporouspermeabletinibasedalloyscaffoldoncellularbiocompatibility AT vnkhodorenko effectofmodifiedsurfaceandarchitectonicsofporouspermeabletinibasedalloyscaffoldoncellularbiocompatibility AT gtdambaev effectofmodifiedsurfaceandarchitectonicsofporouspermeabletinibasedalloyscaffoldoncellularbiocompatibility
_version_	1718416970581278720

Effect of modified surface and architectonics of porous-permeable TiNi-based alloy scaffold on cellular biocompatibility

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