Conserved alternative splicing and expression patterns of arthropod N-cadherin.

Metazoan development requires complex mechanisms to generate cells with diverse function. Alternative splicing of pre-mRNA not only expands proteomic diversity but also provides a means to regulate tissue-specific molecular expression. The N-Cadherin gene in Drosophila contains three pairs of mutual...

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Autores principales:	Shu-Ning Hsu, Shinichi Yonekura, Chun-Yuan Ting, Hugh M Robertson, Youichi Iwai, Tadashi Uemura, Chi-Hon Lee, Akira Chiba
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2009
Materias:	Genetics QH426-470
Acceso en línea:	https://doaj.org/article/b5e85271cfc7467fa589af2388d98c11
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spelling	oai:doaj.org-article:b5e85271cfc7467fa589af2388d98c112021-12-02T20:03:10ZConserved alternative splicing and expression patterns of arthropod N-cadherin.1553-73901553-740410.1371/journal.pgen.1000441https://doaj.org/article/b5e85271cfc7467fa589af2388d98c112009-04-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/19343204/?tool=EBIhttps://doaj.org/toc/1553-7390https://doaj.org/toc/1553-7404Metazoan development requires complex mechanisms to generate cells with diverse function. Alternative splicing of pre-mRNA not only expands proteomic diversity but also provides a means to regulate tissue-specific molecular expression. The N-Cadherin gene in Drosophila contains three pairs of mutually-exclusive alternatively-spliced exons (MEs). However, no significant differences among the resulting protein isoforms have been successfully demonstrated in vivo. Furthermore, while the N-Cadherin gene products exhibit a complex spatiotemporal expression pattern within embryos, its underlying mechanisms and significance remain unknown. Here, we present results that suggest a critical role for alternative splicing in producing a crucial and reproducible complexity in the expression pattern of arthropod N-Cadherin. We demonstrate that the arthropod N-Cadherin gene has maintained the three sets of MEs for over 400 million years using in silico and in vivo approaches. Expression of isoforms derived from these MEs receives precise spatiotemporal control critical during development. Both Drosophila and Tribolium use ME-13a and ME-13b in "neural" and "mesodermal" splice variants, respectively. As proteins, either ME-13a- or ME-13b-containing isoform can cell-autonomously rescue the embryonic lethality caused by genetic loss of N-Cadherin. Ectopic muscle expression of either isoform beyond the time it normally ceases leads to paralysis and lethality. Together, our results offer an example of well-conserved alternative splicing increasing cellular diversity in metazoans.Shu-Ning HsuShinichi YonekuraChun-Yuan TingHugh M RobertsonYouichi IwaiTadashi UemuraChi-Hon LeeAkira ChibaPublic Library of Science (PLoS)articleGeneticsQH426-470ENPLoS Genetics, Vol 5, Iss 4, p e1000441 (2009)
institution	DOAJ
collection	DOAJ
language	EN
topic	Genetics QH426-470
spellingShingle	Genetics QH426-470 Shu-Ning Hsu Shinichi Yonekura Chun-Yuan Ting Hugh M Robertson Youichi Iwai Tadashi Uemura Chi-Hon Lee Akira Chiba Conserved alternative splicing and expression patterns of arthropod N-cadherin.
description	Metazoan development requires complex mechanisms to generate cells with diverse function. Alternative splicing of pre-mRNA not only expands proteomic diversity but also provides a means to regulate tissue-specific molecular expression. The N-Cadherin gene in Drosophila contains three pairs of mutually-exclusive alternatively-spliced exons (MEs). However, no significant differences among the resulting protein isoforms have been successfully demonstrated in vivo. Furthermore, while the N-Cadherin gene products exhibit a complex spatiotemporal expression pattern within embryos, its underlying mechanisms and significance remain unknown. Here, we present results that suggest a critical role for alternative splicing in producing a crucial and reproducible complexity in the expression pattern of arthropod N-Cadherin. We demonstrate that the arthropod N-Cadherin gene has maintained the three sets of MEs for over 400 million years using in silico and in vivo approaches. Expression of isoforms derived from these MEs receives precise spatiotemporal control critical during development. Both Drosophila and Tribolium use ME-13a and ME-13b in "neural" and "mesodermal" splice variants, respectively. As proteins, either ME-13a- or ME-13b-containing isoform can cell-autonomously rescue the embryonic lethality caused by genetic loss of N-Cadherin. Ectopic muscle expression of either isoform beyond the time it normally ceases leads to paralysis and lethality. Together, our results offer an example of well-conserved alternative splicing increasing cellular diversity in metazoans.
format	article
author	Shu-Ning Hsu Shinichi Yonekura Chun-Yuan Ting Hugh M Robertson Youichi Iwai Tadashi Uemura Chi-Hon Lee Akira Chiba
author_facet	Shu-Ning Hsu Shinichi Yonekura Chun-Yuan Ting Hugh M Robertson Youichi Iwai Tadashi Uemura Chi-Hon Lee Akira Chiba
author_sort	Shu-Ning Hsu
title	Conserved alternative splicing and expression patterns of arthropod N-cadherin.
title_short	Conserved alternative splicing and expression patterns of arthropod N-cadherin.
title_full	Conserved alternative splicing and expression patterns of arthropod N-cadherin.
title_fullStr	Conserved alternative splicing and expression patterns of arthropod N-cadherin.
title_full_unstemmed	Conserved alternative splicing and expression patterns of arthropod N-cadherin.
title_sort	conserved alternative splicing and expression patterns of arthropod n-cadherin.
publisher	Public Library of Science (PLoS)
publishDate	2009
url	https://doaj.org/article/b5e85271cfc7467fa589af2388d98c11
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_version_	1718375681515061248

Conserved alternative splicing and expression patterns of arthropod N-cadherin.

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