Molecular Epidemiology of Multidrug-Resistant Uropathogenic <i>Escherichia coli</i> O25b Strains Associated with Complicated Urinary Tract Infection in Children

Background: Uropathogenic <i>Escherichia coli</i> (UPEC) has increased the incidence of urinary tract infection (UTI). It is the cause of more than 80% of community-acquired cystitis cases and more than 70% of uncomplicated acute pyelonephritis cases. Aim: The present study describes the...

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Autores principales:	Laura M. Contreras-Alvarado, Sergio Zavala-Vega, Ariadnna Cruz-Córdova, Juan Pablo Reyes-Grajeda, Gerardo Escalona-Venegas, Víctor Flores, Virginia Alcázar-López, José Arellano-Galindo, Rigoberto Hernández-Castro, Graciela Castro-Escarpulli, Juan Xicohtencatl-Cortes, Sara A. Ochoa
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	UPEC O25 MDR MLST genetic diversity Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/b5eba9a88ff54e6c803246ecbb108e5f
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spelling	oai:doaj.org-article:b5eba9a88ff54e6c803246ecbb108e5f2021-11-25T18:24:56ZMolecular Epidemiology of Multidrug-Resistant Uropathogenic <i>Escherichia coli</i> O25b Strains Associated with Complicated Urinary Tract Infection in Children10.3390/microorganisms91122992076-2607https://doaj.org/article/b5eba9a88ff54e6c803246ecbb108e5f2021-11-01T00:00:00Zhttps://www.mdpi.com/2076-2607/9/11/2299https://doaj.org/toc/2076-2607Background: Uropathogenic <i>Escherichia coli</i> (UPEC) has increased the incidence of urinary tract infection (UTI). It is the cause of more than 80% of community-acquired cystitis cases and more than 70% of uncomplicated acute pyelonephritis cases. Aim: The present study describes the molecular epidemiology of UPEC O25b clinical strains based on their resistance profiles, virulence genes, and genetic diversity. Methods: Resistance profiles were identified using the Kirby–Bauer method, including the phenotypic production of extended-spectrum β-lactamases (ESBLs) and metallo-β-lactamases (MBLs). The UPEC serogroups, phylogenetic groups, virulence genes, and integrons were determined via multiplex PCR. Genetic diversity was established using pulsed-field gel electrophoresis (PFGE), and sequence type (ST) was determined via multilocus sequence typing (MLST). Results: UPEC strains (<i>n</i> = 126) from hospitalized children with complicated UTIs (cUTIs) were identified as O25b, of which 41.27% were multidrug resistant (MDR) and 15.87% were extensively drug resistant (XDR). The O25b strains harbored the <i>fimH</i> (95.23%), <i>csgA</i> (91.26%), <i>papG</i>II (80.95%), <i>chuA</i> (95.23%), <i>iutD</i> (88.09%), <i>satA</i> (84.92%), and <i>intl1</i> (47.61%) genes. Moreover, 64.28% were producers of ESBLs and had high genetic diversity. ST131 (63.63%) was associated primarily with phylogenetic group B2, and ST69 (100%) was associated primarily with phylogenetic group D. Conclusion: UPEC O25b/ST131 harbors a wide genetic diversity of virulence and resistance genes, which contribute to cUTIs in pediatrics.Laura M. Contreras-AlvaradoSergio Zavala-VegaAriadnna Cruz-CórdovaJuan Pablo Reyes-GrajedaGerardo Escalona-VenegasVíctor FloresVirginia Alcázar-LópezJosé Arellano-GalindoRigoberto Hernández-CastroGraciela Castro-EscarpulliJuan Xicohtencatl-CortesSara A. OchoaMDPI AGarticleUPEC O25MDRMLSTgenetic diversityBiology (General)QH301-705.5ENMicroorganisms, Vol 9, Iss 2299, p 2299 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	UPEC O25 MDR MLST genetic diversity Biology (General) QH301-705.5
spellingShingle	UPEC O25 MDR MLST genetic diversity Biology (General) QH301-705.5 Laura M. Contreras-Alvarado Sergio Zavala-Vega Ariadnna Cruz-Córdova Juan Pablo Reyes-Grajeda Gerardo Escalona-Venegas Víctor Flores Virginia Alcázar-López José Arellano-Galindo Rigoberto Hernández-Castro Graciela Castro-Escarpulli Juan Xicohtencatl-Cortes Sara A. Ochoa Molecular Epidemiology of Multidrug-Resistant Uropathogenic <i>Escherichia coli</i> O25b Strains Associated with Complicated Urinary Tract Infection in Children
description	Background: Uropathogenic <i>Escherichia coli</i> (UPEC) has increased the incidence of urinary tract infection (UTI). It is the cause of more than 80% of community-acquired cystitis cases and more than 70% of uncomplicated acute pyelonephritis cases. Aim: The present study describes the molecular epidemiology of UPEC O25b clinical strains based on their resistance profiles, virulence genes, and genetic diversity. Methods: Resistance profiles were identified using the Kirby–Bauer method, including the phenotypic production of extended-spectrum β-lactamases (ESBLs) and metallo-β-lactamases (MBLs). The UPEC serogroups, phylogenetic groups, virulence genes, and integrons were determined via multiplex PCR. Genetic diversity was established using pulsed-field gel electrophoresis (PFGE), and sequence type (ST) was determined via multilocus sequence typing (MLST). Results: UPEC strains (<i>n</i> = 126) from hospitalized children with complicated UTIs (cUTIs) were identified as O25b, of which 41.27% were multidrug resistant (MDR) and 15.87% were extensively drug resistant (XDR). The O25b strains harbored the <i>fimH</i> (95.23%), <i>csgA</i> (91.26%), <i>papG</i>II (80.95%), <i>chuA</i> (95.23%), <i>iutD</i> (88.09%), <i>satA</i> (84.92%), and <i>intl1</i> (47.61%) genes. Moreover, 64.28% were producers of ESBLs and had high genetic diversity. ST131 (63.63%) was associated primarily with phylogenetic group B2, and ST69 (100%) was associated primarily with phylogenetic group D. Conclusion: UPEC O25b/ST131 harbors a wide genetic diversity of virulence and resistance genes, which contribute to cUTIs in pediatrics.
format	article
author	Laura M. Contreras-Alvarado Sergio Zavala-Vega Ariadnna Cruz-Córdova Juan Pablo Reyes-Grajeda Gerardo Escalona-Venegas Víctor Flores Virginia Alcázar-López José Arellano-Galindo Rigoberto Hernández-Castro Graciela Castro-Escarpulli Juan Xicohtencatl-Cortes Sara A. Ochoa
author_facet	Laura M. Contreras-Alvarado Sergio Zavala-Vega Ariadnna Cruz-Córdova Juan Pablo Reyes-Grajeda Gerardo Escalona-Venegas Víctor Flores Virginia Alcázar-López José Arellano-Galindo Rigoberto Hernández-Castro Graciela Castro-Escarpulli Juan Xicohtencatl-Cortes Sara A. Ochoa
author_sort	Laura M. Contreras-Alvarado
title	Molecular Epidemiology of Multidrug-Resistant Uropathogenic <i>Escherichia coli</i> O25b Strains Associated with Complicated Urinary Tract Infection in Children
title_short	Molecular Epidemiology of Multidrug-Resistant Uropathogenic <i>Escherichia coli</i> O25b Strains Associated with Complicated Urinary Tract Infection in Children
title_full	Molecular Epidemiology of Multidrug-Resistant Uropathogenic <i>Escherichia coli</i> O25b Strains Associated with Complicated Urinary Tract Infection in Children
title_fullStr	Molecular Epidemiology of Multidrug-Resistant Uropathogenic <i>Escherichia coli</i> O25b Strains Associated with Complicated Urinary Tract Infection in Children
title_full_unstemmed	Molecular Epidemiology of Multidrug-Resistant Uropathogenic <i>Escherichia coli</i> O25b Strains Associated with Complicated Urinary Tract Infection in Children
title_sort	molecular epidemiology of multidrug-resistant uropathogenic <i>escherichia coli</i> o25b strains associated with complicated urinary tract infection in children
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/b5eba9a88ff54e6c803246ecbb108e5f
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Molecular Epidemiology of Multidrug-Resistant Uropathogenic <i>Escherichia coli</i> O25b Strains Associated with Complicated Urinary Tract Infection in Children

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