An epithelial-to-mesenchymal transition-inducing potential of granulocyte macrophage colony-stimulating factor in colon cancer

Abstract Growing evidence shows that granulocyte macrophage colony-stimulating factor (GM-CSF) has progression-promoting potentials in certain solid tumors, which is largely attributed to the immunomodulatory function of this cytokine in tumor niches. However, little is known about the effect of GM-...

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Autores principales:	Yaqiong Chen, Zhi Zhao, Yu Chen, Zhonglin Lv, Xin Ding, Renxi Wang, He Xiao, Chunmei Hou, Beifen Shen, Jiannan Feng, Renfeng Guo, Yan Li, Hui Peng, Gencheng Han, Guojiang Chen
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/b5f035520ae64b008032050e02b14f85
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spelling	oai:doaj.org-article:b5f035520ae64b008032050e02b14f852021-12-02T11:52:56ZAn epithelial-to-mesenchymal transition-inducing potential of granulocyte macrophage colony-stimulating factor in colon cancer10.1038/s41598-017-08047-12045-2322https://doaj.org/article/b5f035520ae64b008032050e02b14f852017-08-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-08047-1https://doaj.org/toc/2045-2322Abstract Growing evidence shows that granulocyte macrophage colony-stimulating factor (GM-CSF) has progression-promoting potentials in certain solid tumors, which is largely attributed to the immunomodulatory function of this cytokine in tumor niches. However, little is known about the effect of GM-CSF on cancer cells. Herein, we show that chronic exposure of colon cancer cells to GM-CSF, which harbor its receptor, leads to occurrence of epithelial to mesenchymal transition (EMT), in time and dose-dependent manners. These GM-CSF-educated cancer cells exhibit enhanced ability of motility in vitro and in vivo. Furthermore, GM-CSF stimulation renders colon cancer cells more resistant to cytotoxic agents. Mechanistic investigation reveals that MAPK/ERK signaling and EMT-inducing transcription factor ZEB1 are critical to mediate these effects of GM-CSF. In specimen of CRC patients, high-level expression of GM-CSF positively correlates with local metastases in lymph nodes. Moreover, the co-expression of GM-CSF and its receptors as well as phosphorylated ERK1/2 are observed. Thus, our study for the first time identifies a progression-promoting function of GM-CSF in colon cancer by inducing EMT.Yaqiong ChenZhi ZhaoYu ChenZhonglin LvXin DingRenxi WangHe XiaoChunmei HouBeifen ShenJiannan FengRenfeng GuoYan LiHui PengGencheng HanGuojiang ChenNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-12 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Yaqiong Chen Zhi Zhao Yu Chen Zhonglin Lv Xin Ding Renxi Wang He Xiao Chunmei Hou Beifen Shen Jiannan Feng Renfeng Guo Yan Li Hui Peng Gencheng Han Guojiang Chen An epithelial-to-mesenchymal transition-inducing potential of granulocyte macrophage colony-stimulating factor in colon cancer
description	Abstract Growing evidence shows that granulocyte macrophage colony-stimulating factor (GM-CSF) has progression-promoting potentials in certain solid tumors, which is largely attributed to the immunomodulatory function of this cytokine in tumor niches. However, little is known about the effect of GM-CSF on cancer cells. Herein, we show that chronic exposure of colon cancer cells to GM-CSF, which harbor its receptor, leads to occurrence of epithelial to mesenchymal transition (EMT), in time and dose-dependent manners. These GM-CSF-educated cancer cells exhibit enhanced ability of motility in vitro and in vivo. Furthermore, GM-CSF stimulation renders colon cancer cells more resistant to cytotoxic agents. Mechanistic investigation reveals that MAPK/ERK signaling and EMT-inducing transcription factor ZEB1 are critical to mediate these effects of GM-CSF. In specimen of CRC patients, high-level expression of GM-CSF positively correlates with local metastases in lymph nodes. Moreover, the co-expression of GM-CSF and its receptors as well as phosphorylated ERK1/2 are observed. Thus, our study for the first time identifies a progression-promoting function of GM-CSF in colon cancer by inducing EMT.
format	article
author	Yaqiong Chen Zhi Zhao Yu Chen Zhonglin Lv Xin Ding Renxi Wang He Xiao Chunmei Hou Beifen Shen Jiannan Feng Renfeng Guo Yan Li Hui Peng Gencheng Han Guojiang Chen
author_facet	Yaqiong Chen Zhi Zhao Yu Chen Zhonglin Lv Xin Ding Renxi Wang He Xiao Chunmei Hou Beifen Shen Jiannan Feng Renfeng Guo Yan Li Hui Peng Gencheng Han Guojiang Chen
author_sort	Yaqiong Chen
title	An epithelial-to-mesenchymal transition-inducing potential of granulocyte macrophage colony-stimulating factor in colon cancer
title_short	An epithelial-to-mesenchymal transition-inducing potential of granulocyte macrophage colony-stimulating factor in colon cancer
title_full	An epithelial-to-mesenchymal transition-inducing potential of granulocyte macrophage colony-stimulating factor in colon cancer
title_fullStr	An epithelial-to-mesenchymal transition-inducing potential of granulocyte macrophage colony-stimulating factor in colon cancer
title_full_unstemmed	An epithelial-to-mesenchymal transition-inducing potential of granulocyte macrophage colony-stimulating factor in colon cancer
title_sort	epithelial-to-mesenchymal transition-inducing potential of granulocyte macrophage colony-stimulating factor in colon cancer
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/b5f035520ae64b008032050e02b14f85
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An epithelial-to-mesenchymal transition-inducing potential of granulocyte macrophage colony-stimulating factor in colon cancer

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