High-throughput analysis of the satellitome revealed enormous diversity of satellite DNAs in the neo-Y chromosome of the cricket Eneoptera surinamensis

Abstract Satellite DNAs (satDNAs) constitute large portion of eukaryote genomes, comprising non-protein-coding sequences tandemly repeated. They are mostly found in heterochromatic regions of chromosomes such as around centromere or near telomeres, in intercalary heterochromatin, and often in non-re...

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Autores principales:	Octavio Manuel Palacios-Gimenez, Guilherme Borges Dias, Leonardo Gomes de Lima, Gustavo Campos e Silva Kuhn, Érica Ramos, Cesar Martins, Diogo Cavalcanti Cabral-de-Mello
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/b5f8a9511a3740098741f054d050a975
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spelling	oai:doaj.org-article:b5f8a9511a3740098741f054d050a9752021-12-02T16:06:56ZHigh-throughput analysis of the satellitome revealed enormous diversity of satellite DNAs in the neo-Y chromosome of the cricket Eneoptera surinamensis10.1038/s41598-017-06822-82045-2322https://doaj.org/article/b5f8a9511a3740098741f054d050a9752017-07-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-06822-8https://doaj.org/toc/2045-2322Abstract Satellite DNAs (satDNAs) constitute large portion of eukaryote genomes, comprising non-protein-coding sequences tandemly repeated. They are mostly found in heterochromatic regions of chromosomes such as around centromere or near telomeres, in intercalary heterochromatin, and often in non-recombining segments of sex chromosomes. We examined the satellitome in the cricket Eneoptera surinamensis (2n = 9, neo-X1X2Y, males) to characterize the molecular evolution of its neo-sex chromosomes. To achieve this, we analyzed illumina reads using graph-based clustering and complementary analyses. We found an unusually high number of 45 families of satDNAs, ranging from 4 bp to 517 bp, accounting for about 14% of the genome and showing different modular structures and high diversity of arrays. FISH mapping revealed that satDNAs are located mostly in C-positive pericentromeric regions of the chromosomes. SatDNAs enrichment was also observed in the neo-sex chromosomes in comparison to autosomes. Especially astonishing accumulation of satDNAs loci was found in the highly differentiated neo-Y, including 39 satDNAs over-represented in this chromosome, which is the greatest satDNAs diversity yet reported for sex chromosomes. Our results suggest possible involvement of satDNAs in genome increasing and in molecular differentiation of the neo-sex chromosomes in this species, contributing to the understanding of sex chromosome composition and evolution in Orthoptera.Octavio Manuel Palacios-GimenezGuilherme Borges DiasLeonardo Gomes de LimaGustavo Campos e Silva KuhnÉrica RamosCesar MartinsDiogo Cavalcanti Cabral-de-MelloNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-11 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Octavio Manuel Palacios-Gimenez Guilherme Borges Dias Leonardo Gomes de Lima Gustavo Campos e Silva Kuhn Érica Ramos Cesar Martins Diogo Cavalcanti Cabral-de-Mello High-throughput analysis of the satellitome revealed enormous diversity of satellite DNAs in the neo-Y chromosome of the cricket Eneoptera surinamensis
description	Abstract Satellite DNAs (satDNAs) constitute large portion of eukaryote genomes, comprising non-protein-coding sequences tandemly repeated. They are mostly found in heterochromatic regions of chromosomes such as around centromere or near telomeres, in intercalary heterochromatin, and often in non-recombining segments of sex chromosomes. We examined the satellitome in the cricket Eneoptera surinamensis (2n = 9, neo-X1X2Y, males) to characterize the molecular evolution of its neo-sex chromosomes. To achieve this, we analyzed illumina reads using graph-based clustering and complementary analyses. We found an unusually high number of 45 families of satDNAs, ranging from 4 bp to 517 bp, accounting for about 14% of the genome and showing different modular structures and high diversity of arrays. FISH mapping revealed that satDNAs are located mostly in C-positive pericentromeric regions of the chromosomes. SatDNAs enrichment was also observed in the neo-sex chromosomes in comparison to autosomes. Especially astonishing accumulation of satDNAs loci was found in the highly differentiated neo-Y, including 39 satDNAs over-represented in this chromosome, which is the greatest satDNAs diversity yet reported for sex chromosomes. Our results suggest possible involvement of satDNAs in genome increasing and in molecular differentiation of the neo-sex chromosomes in this species, contributing to the understanding of sex chromosome composition and evolution in Orthoptera.
format	article
author	Octavio Manuel Palacios-Gimenez Guilherme Borges Dias Leonardo Gomes de Lima Gustavo Campos e Silva Kuhn Érica Ramos Cesar Martins Diogo Cavalcanti Cabral-de-Mello
author_facet	Octavio Manuel Palacios-Gimenez Guilherme Borges Dias Leonardo Gomes de Lima Gustavo Campos e Silva Kuhn Érica Ramos Cesar Martins Diogo Cavalcanti Cabral-de-Mello
author_sort	Octavio Manuel Palacios-Gimenez
title	High-throughput analysis of the satellitome revealed enormous diversity of satellite DNAs in the neo-Y chromosome of the cricket Eneoptera surinamensis
title_short	High-throughput analysis of the satellitome revealed enormous diversity of satellite DNAs in the neo-Y chromosome of the cricket Eneoptera surinamensis
title_full	High-throughput analysis of the satellitome revealed enormous diversity of satellite DNAs in the neo-Y chromosome of the cricket Eneoptera surinamensis
title_fullStr	High-throughput analysis of the satellitome revealed enormous diversity of satellite DNAs in the neo-Y chromosome of the cricket Eneoptera surinamensis
title_full_unstemmed	High-throughput analysis of the satellitome revealed enormous diversity of satellite DNAs in the neo-Y chromosome of the cricket Eneoptera surinamensis
title_sort	high-throughput analysis of the satellitome revealed enormous diversity of satellite dnas in the neo-y chromosome of the cricket eneoptera surinamensis
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/b5f8a9511a3740098741f054d050a975
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High-throughput analysis of the satellitome revealed enormous diversity of satellite DNAs in the neo-Y chromosome of the cricket Eneoptera surinamensis

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