Extracellular vesicles isolated from milk can improve gut barrier dysfunction induced by malnutrition

Abstract Malnutrition impacts approximately 50 million children worldwide and is linked to 45% of global mortality in children below the age of five. Severe acute malnutrition (SAM) is associated with intestinal barrier breakdown and epithelial atrophy. Extracellular vesicles including exosomes (EVs...

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Autores principales:	Mohamed Karim Maghraby, Bo Li, Lijun Chi, Catriona Ling, Abderrahim Benmoussa, Patrick Provost, Andrea C. Postmus, Abdirahman Abdi, Agostino Pierro, Celine Bourdon, Robert H. J. Bandsma
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/b60688e4190a4b43bfa850b3d11dbccf
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spelling	oai:doaj.org-article:b60688e4190a4b43bfa850b3d11dbccf2021-12-02T14:37:21ZExtracellular vesicles isolated from milk can improve gut barrier dysfunction induced by malnutrition10.1038/s41598-021-86920-w2045-2322https://doaj.org/article/b60688e4190a4b43bfa850b3d11dbccf2021-04-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-86920-whttps://doaj.org/toc/2045-2322Abstract Malnutrition impacts approximately 50 million children worldwide and is linked to 45% of global mortality in children below the age of five. Severe acute malnutrition (SAM) is associated with intestinal barrier breakdown and epithelial atrophy. Extracellular vesicles including exosomes (EVs; 30–150 nm) can travel to distant target cells through biofluids including milk. Since milk-derived EVs are known to induce intestinal stem cell proliferation, this study aimed to examine their potential efficacy in improving malnutrition-induced atrophy of intestinal mucosa and barrier dysfunction. Mice were fed either a control (18%) or a low protein (1%) diet for 14 days to induce malnutrition. From day 10 to 14, they received either bovine milk EVs or control gavage and were sacrificed on day 15, 4 h after a Fluorescein Isothiocyanate (FITC) dose. Tissue and blood were collected for histological and epithelial barrier function analyses. Mice fed low protein diet developed intestinal villus atrophy and barrier dysfunction. Despite continued low protein diet feeding, milk EV treatment improved intestinal permeability, intestinal architecture and cellular proliferation. Our results suggest that EVs enriched from milk should be further explored as a valuable adjuvant therapy to standard clinical management of malnourished children with high risk of morbidity and mortality.Mohamed Karim MaghrabyBo LiLijun ChiCatriona LingAbderrahim BenmoussaPatrick ProvostAndrea C. PostmusAbdirahman AbdiAgostino PierroCeline BourdonRobert H. J. BandsmaNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-14 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Mohamed Karim Maghraby Bo Li Lijun Chi Catriona Ling Abderrahim Benmoussa Patrick Provost Andrea C. Postmus Abdirahman Abdi Agostino Pierro Celine Bourdon Robert H. J. Bandsma Extracellular vesicles isolated from milk can improve gut barrier dysfunction induced by malnutrition
description	Abstract Malnutrition impacts approximately 50 million children worldwide and is linked to 45% of global mortality in children below the age of five. Severe acute malnutrition (SAM) is associated with intestinal barrier breakdown and epithelial atrophy. Extracellular vesicles including exosomes (EVs; 30–150 nm) can travel to distant target cells through biofluids including milk. Since milk-derived EVs are known to induce intestinal stem cell proliferation, this study aimed to examine their potential efficacy in improving malnutrition-induced atrophy of intestinal mucosa and barrier dysfunction. Mice were fed either a control (18%) or a low protein (1%) diet for 14 days to induce malnutrition. From day 10 to 14, they received either bovine milk EVs or control gavage and were sacrificed on day 15, 4 h after a Fluorescein Isothiocyanate (FITC) dose. Tissue and blood were collected for histological and epithelial barrier function analyses. Mice fed low protein diet developed intestinal villus atrophy and barrier dysfunction. Despite continued low protein diet feeding, milk EV treatment improved intestinal permeability, intestinal architecture and cellular proliferation. Our results suggest that EVs enriched from milk should be further explored as a valuable adjuvant therapy to standard clinical management of malnourished children with high risk of morbidity and mortality.
format	article
author	Mohamed Karim Maghraby Bo Li Lijun Chi Catriona Ling Abderrahim Benmoussa Patrick Provost Andrea C. Postmus Abdirahman Abdi Agostino Pierro Celine Bourdon Robert H. J. Bandsma
author_facet	Mohamed Karim Maghraby Bo Li Lijun Chi Catriona Ling Abderrahim Benmoussa Patrick Provost Andrea C. Postmus Abdirahman Abdi Agostino Pierro Celine Bourdon Robert H. J. Bandsma
author_sort	Mohamed Karim Maghraby
title	Extracellular vesicles isolated from milk can improve gut barrier dysfunction induced by malnutrition
title_short	Extracellular vesicles isolated from milk can improve gut barrier dysfunction induced by malnutrition
title_full	Extracellular vesicles isolated from milk can improve gut barrier dysfunction induced by malnutrition
title_fullStr	Extracellular vesicles isolated from milk can improve gut barrier dysfunction induced by malnutrition
title_full_unstemmed	Extracellular vesicles isolated from milk can improve gut barrier dysfunction induced by malnutrition
title_sort	extracellular vesicles isolated from milk can improve gut barrier dysfunction induced by malnutrition
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/b60688e4190a4b43bfa850b3d11dbccf
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Extracellular vesicles isolated from milk can improve gut barrier dysfunction induced by malnutrition

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