Analytical Validation of a New Immunoenzymatic Method for the Measurement of Feline Parathyroid Hormone in Cats with Chronic Kidney Disease

The determination of parathyroid hormone (PTH) in cats could be of clinical utility in many metabolic disorders, such as renal diseases, hypercalcemia, or nutritional imbalances. However, the available methods for the measurement of feline PTH are limited, not widely available, and need radioimmunoa...

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Autores principales:	Jari Zambarbieri, Pierangelo Moretti, Alessia Giordano, Paola Scarpa
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	parathyroid hormone cat validation Veterinary medicine SF600-1100 Zoology QL1-991
Acceso en línea:	https://doaj.org/article/b6098ba8ac7f4ce2a816e98f01f005ef
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spelling	oai:doaj.org-article:b6098ba8ac7f4ce2a816e98f01f005ef2021-11-25T16:15:20ZAnalytical Validation of a New Immunoenzymatic Method for the Measurement of Feline Parathyroid Hormone in Cats with Chronic Kidney Disease10.3390/ani111131002076-2615https://doaj.org/article/b6098ba8ac7f4ce2a816e98f01f005ef2021-10-01T00:00:00Zhttps://www.mdpi.com/2076-2615/11/11/3100https://doaj.org/toc/2076-2615The determination of parathyroid hormone (PTH) in cats could be of clinical utility in many metabolic disorders, such as renal diseases, hypercalcemia, or nutritional imbalances. However, the available methods for the measurement of feline PTH are limited, not widely available, and need radioimmunoassays. The aim of this study was to perform the analytical validation of a new immunoenzymatic method for the measurement of feline PTH. Thirty-eight cats affected with chronic kidney disease (CKD) were included. PTH was measured using a two-site immunoenzymatic method validated in humans and dogs (ST AIA-PACK<sup>®</sup> Intact PTH, Tosoh Bioscience, Tessenderlo, Belgium). The analytical validation provided the evaluation of precision (intra-assay and inter-assay), accuracy (linearity under dilution (LUD) and spike recovery test (SRT)), and the storage stability of serum samples at 20 °C, 4 °C, and −20 °C. The method showed good precision (intra-assay CVs (coefficient of variations) 3.19–9.61%; inter-assay CVs 9.26–15.28%). In both the intra- and inter-assays, the highest imprecision was found with the low concentration pool (9.61% and 15.28%) and accuracy (LUD and SRT r<sup>2</sup> = 0.99, <i>p</i> < 0.001), while the stability was optimal up until 7 days at −20 °C (−7.7%). The method was successfully validated in cats, allowing its future use in diagnostic procedures.Jari ZambarbieriPierangelo MorettiAlessia GiordanoPaola ScarpaMDPI AGarticleparathyroid hormonecatvalidationVeterinary medicineSF600-1100ZoologyQL1-991ENAnimals, Vol 11, Iss 3100, p 3100 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	parathyroid hormone cat validation Veterinary medicine SF600-1100 Zoology QL1-991
spellingShingle	parathyroid hormone cat validation Veterinary medicine SF600-1100 Zoology QL1-991 Jari Zambarbieri Pierangelo Moretti Alessia Giordano Paola Scarpa Analytical Validation of a New Immunoenzymatic Method for the Measurement of Feline Parathyroid Hormone in Cats with Chronic Kidney Disease
description	The determination of parathyroid hormone (PTH) in cats could be of clinical utility in many metabolic disorders, such as renal diseases, hypercalcemia, or nutritional imbalances. However, the available methods for the measurement of feline PTH are limited, not widely available, and need radioimmunoassays. The aim of this study was to perform the analytical validation of a new immunoenzymatic method for the measurement of feline PTH. Thirty-eight cats affected with chronic kidney disease (CKD) were included. PTH was measured using a two-site immunoenzymatic method validated in humans and dogs (ST AIA-PACK<sup>®</sup> Intact PTH, Tosoh Bioscience, Tessenderlo, Belgium). The analytical validation provided the evaluation of precision (intra-assay and inter-assay), accuracy (linearity under dilution (LUD) and spike recovery test (SRT)), and the storage stability of serum samples at 20 °C, 4 °C, and −20 °C. The method showed good precision (intra-assay CVs (coefficient of variations) 3.19–9.61%; inter-assay CVs 9.26–15.28%). In both the intra- and inter-assays, the highest imprecision was found with the low concentration pool (9.61% and 15.28%) and accuracy (LUD and SRT r<sup>2</sup> = 0.99, <i>p</i> < 0.001), while the stability was optimal up until 7 days at −20 °C (−7.7%). The method was successfully validated in cats, allowing its future use in diagnostic procedures.
format	article
author	Jari Zambarbieri Pierangelo Moretti Alessia Giordano Paola Scarpa
author_facet	Jari Zambarbieri Pierangelo Moretti Alessia Giordano Paola Scarpa
author_sort	Jari Zambarbieri
title	Analytical Validation of a New Immunoenzymatic Method for the Measurement of Feline Parathyroid Hormone in Cats with Chronic Kidney Disease
title_short	Analytical Validation of a New Immunoenzymatic Method for the Measurement of Feline Parathyroid Hormone in Cats with Chronic Kidney Disease
title_full	Analytical Validation of a New Immunoenzymatic Method for the Measurement of Feline Parathyroid Hormone in Cats with Chronic Kidney Disease
title_fullStr	Analytical Validation of a New Immunoenzymatic Method for the Measurement of Feline Parathyroid Hormone in Cats with Chronic Kidney Disease
title_full_unstemmed	Analytical Validation of a New Immunoenzymatic Method for the Measurement of Feline Parathyroid Hormone in Cats with Chronic Kidney Disease
title_sort	analytical validation of a new immunoenzymatic method for the measurement of feline parathyroid hormone in cats with chronic kidney disease
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/b6098ba8ac7f4ce2a816e98f01f005ef
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Analytical Validation of a New Immunoenzymatic Method for the Measurement of Feline Parathyroid Hormone in Cats with Chronic Kidney Disease

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