Characterizing bulk rigidity of rigid red blood cell populations in sickle-cell disease patients

Abstract In this work, we utilized a parameterization model of ektacytometry to quantify the bulk rigidity of the rigid red blood cell (RBC) population in sickle cell disease (SCD) patients. Current ektacytometry techniques implement laser diffraction viscometry to estimate the RBC deformability in...

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Autores principales:	Mario Gutierrez, Mark Shamoun, Katie Giger Seu, Tyler Tanski, Theodosia A. Kalfa, Omolola Eniola-Adefeso
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/b60ad72e48f943938f60ea1c478716f4
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spelling	oai:doaj.org-article:b60ad72e48f943938f60ea1c478716f42021-12-02T14:27:53ZCharacterizing bulk rigidity of rigid red blood cell populations in sickle-cell disease patients10.1038/s41598-021-86582-82045-2322https://doaj.org/article/b60ad72e48f943938f60ea1c478716f42021-04-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-86582-8https://doaj.org/toc/2045-2322Abstract In this work, we utilized a parameterization model of ektacytometry to quantify the bulk rigidity of the rigid red blood cell (RBC) population in sickle cell disease (SCD) patients. Current ektacytometry techniques implement laser diffraction viscometry to estimate the RBC deformability in a whole blood sample. However, the diffraction measurement is an average of all cells present in the measured sample. By coupling an existing parameterization model of ektacytometry to an artificially rigid RBC model, we formulated an innovative system for estimating the average rigidity of the rigid RBC population in SCD blood. We demonstrated that this method could more accurately determine the bulk stiffness of the rigid RBC populations. This information could potentially help develop the ektacytometry technique as a tool for assessing disease severity in SCD patients, offering novel insights into the disease pathology and treatment.Mario GutierrezMark ShamounKatie Giger SeuTyler TanskiTheodosia A. KalfaOmolola Eniola-AdefesoNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-10 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Mario Gutierrez Mark Shamoun Katie Giger Seu Tyler Tanski Theodosia A. Kalfa Omolola Eniola-Adefeso Characterizing bulk rigidity of rigid red blood cell populations in sickle-cell disease patients
description	Abstract In this work, we utilized a parameterization model of ektacytometry to quantify the bulk rigidity of the rigid red blood cell (RBC) population in sickle cell disease (SCD) patients. Current ektacytometry techniques implement laser diffraction viscometry to estimate the RBC deformability in a whole blood sample. However, the diffraction measurement is an average of all cells present in the measured sample. By coupling an existing parameterization model of ektacytometry to an artificially rigid RBC model, we formulated an innovative system for estimating the average rigidity of the rigid RBC population in SCD blood. We demonstrated that this method could more accurately determine the bulk stiffness of the rigid RBC populations. This information could potentially help develop the ektacytometry technique as a tool for assessing disease severity in SCD patients, offering novel insights into the disease pathology and treatment.
format	article
author	Mario Gutierrez Mark Shamoun Katie Giger Seu Tyler Tanski Theodosia A. Kalfa Omolola Eniola-Adefeso
author_facet	Mario Gutierrez Mark Shamoun Katie Giger Seu Tyler Tanski Theodosia A. Kalfa Omolola Eniola-Adefeso
author_sort	Mario Gutierrez
title	Characterizing bulk rigidity of rigid red blood cell populations in sickle-cell disease patients
title_short	Characterizing bulk rigidity of rigid red blood cell populations in sickle-cell disease patients
title_full	Characterizing bulk rigidity of rigid red blood cell populations in sickle-cell disease patients
title_fullStr	Characterizing bulk rigidity of rigid red blood cell populations in sickle-cell disease patients
title_full_unstemmed	Characterizing bulk rigidity of rigid red blood cell populations in sickle-cell disease patients
title_sort	characterizing bulk rigidity of rigid red blood cell populations in sickle-cell disease patients
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/b60ad72e48f943938f60ea1c478716f4
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Characterizing bulk rigidity of rigid red blood cell populations in sickle-cell disease patients

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