Improving the DNA specificity and applicability of base editing through protein engineering and protein delivery

Third-generation base editors consist of a catalytically disabled Cas9 fused to a cytidine deaminase and a base excision repair inhibitor, enabling efficient, precise editing of individual base pairs in DNA. Here the authors describe engineering and protein delivery of base editors to improve their...

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Autores principales:	Holly A. Rees, Alexis C. Komor, Wei-Hsi Yeh, Joana Caetano-Lopes, Matthew Warman, Albert S. B. Edge, David R. Liu
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Science Q
Acceso en línea:	https://doaj.org/article/b60c28c60af84bb2b31538ea1a91ab66
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spelling	oai:doaj.org-article:b60c28c60af84bb2b31538ea1a91ab662021-12-02T17:06:07ZImproving the DNA specificity and applicability of base editing through protein engineering and protein delivery10.1038/ncomms157902041-1723https://doaj.org/article/b60c28c60af84bb2b31538ea1a91ab662017-06-01T00:00:00Zhttps://doi.org/10.1038/ncomms15790https://doaj.org/toc/2041-1723Third-generation base editors consist of a catalytically disabled Cas9 fused to a cytidine deaminase and a base excision repair inhibitor, enabling efficient, precise editing of individual base pairs in DNA. Here the authors describe engineering and protein delivery of base editors to improve their DNA specificity and enable specific base editing in live animals.Holly A. ReesAlexis C. KomorWei-Hsi YehJoana Caetano-LopesMatthew WarmanAlbert S. B. EdgeDavid R. LiuNature PortfolioarticleScienceQENNature Communications, Vol 8, Iss 1, Pp 1-10 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Science Q
spellingShingle	Science Q Holly A. Rees Alexis C. Komor Wei-Hsi Yeh Joana Caetano-Lopes Matthew Warman Albert S. B. Edge David R. Liu Improving the DNA specificity and applicability of base editing through protein engineering and protein delivery
description	Third-generation base editors consist of a catalytically disabled Cas9 fused to a cytidine deaminase and a base excision repair inhibitor, enabling efficient, precise editing of individual base pairs in DNA. Here the authors describe engineering and protein delivery of base editors to improve their DNA specificity and enable specific base editing in live animals.
format	article
author	Holly A. Rees Alexis C. Komor Wei-Hsi Yeh Joana Caetano-Lopes Matthew Warman Albert S. B. Edge David R. Liu
author_facet	Holly A. Rees Alexis C. Komor Wei-Hsi Yeh Joana Caetano-Lopes Matthew Warman Albert S. B. Edge David R. Liu
author_sort	Holly A. Rees
title	Improving the DNA specificity and applicability of base editing through protein engineering and protein delivery
title_short	Improving the DNA specificity and applicability of base editing through protein engineering and protein delivery
title_full	Improving the DNA specificity and applicability of base editing through protein engineering and protein delivery
title_fullStr	Improving the DNA specificity and applicability of base editing through protein engineering and protein delivery
title_full_unstemmed	Improving the DNA specificity and applicability of base editing through protein engineering and protein delivery
title_sort	improving the dna specificity and applicability of base editing through protein engineering and protein delivery
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/b60c28c60af84bb2b31538ea1a91ab66
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Improving the DNA specificity and applicability of base editing through protein engineering and protein delivery

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