Complementarity of PALM and SOFI for super-resolution live-cell imaging of focal adhesions

Live cell super-resolution imaging requires a high temporal resolution, which remains a challenge. Here the authors combine photo-activated localization microscopy (PALM) with super-resolution optical fluctuation imaging (SOFI) to achieve high spatiotemporal resolution and quantitative imaging of fo...

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Autores principales: Hendrik Deschout, Tomas Lukes, Azat Sharipov, Daniel Szlag, Lely Feletti, Wim Vandenberg, Peter Dedecker, Johan Hofkens, Marcel Leutenegger, Theo Lasser, Aleksandra Radenovic
Formato: article
Lenguaje:EN
Publicado: Nature Portfolio 2016
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Acceso en línea:https://doaj.org/article/b60dbd46b55d452085ed56fc80de41ea
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Sumario:Live cell super-resolution imaging requires a high temporal resolution, which remains a challenge. Here the authors combine photo-activated localization microscopy (PALM) with super-resolution optical fluctuation imaging (SOFI) to achieve high spatiotemporal resolution and quantitative imaging of focal adhesion dynamics.